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《Acta Agronomica Sinica》 2003-04
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Construction of a Brassica napus Bacterial Artificial Chromosome Library and Identification of Clones Linked to Boron Efficiency Gene

HU Zheng XU Fang Sen ZHAO Jian Wei MENG Jin LingFoundation item:The National 863 High Technology Program of China (2001AA222311) and National Special Foundation for Transgenic Plant (J00A 008 01). Biography:HU Zheng (1977-); Male;Zhejiang Province; Master. * Corresponding author:MENG Jin Ling, E mail:jmeng@mail.hzau.cn Received (收稿日期):2002 09 06;Accepted(接受日期):2003 01 13. (National Key Laboratory of Crop Genetic Improvement, National Molecular Breeding Center, Huazhong Agricultural University, Wuhan, Hubei 430070, China)  
A bacterial artificial chromosome (BAC) library was constructed for a Brassica napus cultivar: Ning RS 1. The library contains 82944 clones with 80kb DNA insert size in average representing equivalents of 5 1 Brassica napus haploid genome. The BAC DNA can maintain stably in E. coli after about 100 generations of culture. pa28, an Arabidopsis EST clone which linked to a boron efficiency gene BE1 in B.napus , was used as probe to screen the library: consequently, 13 positive clones were identified. These positive clones were divided into four groups. Three of 13 clones were identified to link to the BE1 locus by Southern analysis.
【Fund】: TheNational863HighTechnologyProgramofChina ( 2 0 0 1AA2 2 2 3 11);; NationalSpecialFoundationforTransgenicPlant(J0 0A 0 0 8 0 1)
【CateGory Index】: S565.4
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