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《Acta Agronomica Sinica》 2003-04
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Construction of a Brassica napus Bacterial Artificial Chromosome Library and Identification of Clones Linked to Boron Efficiency Gene

HU Zheng XU Fang Sen ZHAO Jian Wei MENG Jin LingFoundation item:The National 863 High Technology Program of China (2001AA222311) and National Special Foundation for Transgenic Plant (J00A 008 01). Biography:HU Zheng (1977-); Male;Zhejiang Province; Master. * Corresponding author:MENG Jin Ling, E mail:jmeng@mail.hzau.cn Received (收稿日期):2002 09 06;Accepted(接受日期):2003 01 13. (National Key Laboratory of Crop Genetic Improvement, National Molecular Breeding Center, Huazhong Agricultural University, Wuhan, Hubei 430070, China)  
A bacterial artificial chromosome (BAC) library was constructed for a Brassica napus cultivar: Ning RS 1. The library contains 82944 clones with 80kb DNA insert size in average representing equivalents of 5 1 Brassica napus haploid genome. The BAC DNA can maintain stably in E. coli after about 100 generations of culture. pa28, an Arabidopsis EST clone which linked to a boron efficiency gene BE1 in B.napus , was used as probe to screen the library: consequently, 13 positive clones were identified. These positive clones were divided into four groups. Three of 13 clones were identified to link to the BE1 locus by Southern analysis.
【Fund】: TheNational863HighTechnologyProgramofChina ( 2 0 0 1AA2 2 2 3 11);; NationalSpecialFoundationforTransgenicPlant(J0 0A 0 0 8 0 1)
【CateGory Index】: S565.4
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Chinese Journal Full-text Database 1 Hits
1 A Xin-Xiang~1, CHENG Zai-Quan~2, WU Cheng-Jun~2, YAN Bo~2, CHEN Shan-Na~1, YANG Ming-Zhi~~1, HUANG Xing-Qi~(2,*) (()~1College of Life Sciences,Yunnan University,Kunming 650091, Yunnan; ()~2Biotechnology Research Institute,Yunnan Academy of Agricultural Sciences, Kunming 650223, Yunnan, China);Construction and Analysis of Bacterial Artificial Chromosome (BAC) Library of Oryza granulata Nees et Arn. ex Watt. in Yunnan[J];Acta Agronomica Sinica;2005-06
Chinese Journal Full-text Database 5 Hits
1 Hu Qiuhui Xu Guangbi Shi Ruihe (Research Laboratory of Plant Nutrition);STUDY ON THE INHERITABLE DIFFERENCE ON BORON NUTRITION OF DIFFERENT CULTIVARS OF OILSEED RAPE[J];Journal of Nanjing Agricultural University;1990-01
2 Shen Kang;Shen Zhen-guo;Xu Han-qing;Huang Qing-yuan Department of Agronomy, Nanjing Agricultural University, Nanjing 210014;Study on Boron Nutrition and Seed Setting of Rapeseed[J];Acta Agronomica Sinica;1993-06
3 WANG Li Xia, ZHAO Jian Wei, XU Fang Sen, LIU Ren Hu, MENG Jin Ling ① (National Key Laboratory of Cenetic Improvement, Huazhong Agricultural Universisity, Wuhan 430070, China);Integration of DNA Clones Related to Important Economic Traits of Brassica napus onto Arabidopsis Genetic Map[J];Acta Genetica Sinica;2002-08
4 ZU Yan qun, LIN Ke hui ( Faculty of Agricultural Science and Technology, Y A U,Kunming 650201,China );The Role of Boron in Plants and its Effect on the Yield and Quality of Crops[J];Journal of Yunnan Agricultural University;2000-04
5 Shen Zhenguo;Zhang Xiusheng;Wang Zhenyu;Shen Kang(Department of Agronomy,Nanjing Agricultural University,Nanjing 210014);ON THE RELATIONSHIP BETWEEN BORON NUTRITION AND DEVELOPMENT OF ANTHER(POLLEN)IN RAPESEED PLANT[J];SCIENTIA AGRICUTURA SINICA;1994-01
Chinese Journal Full-text Database 10 Hits
1 Yang Feng - juan, Liu Shi - qi, Wang Xiu - feng ( Department of Horticulture, Shandong Agricultural University, Taian 271018);Effect of different varieties and trace fertilizers on allicin content in bulb of garlic[J];Shandong Agricultural Sciences;2004-04
2 CHEN Gang et al (College of Resource and Environment, Huazhong Agricultural University,Wuhan,Hubei 430070);Research Advance in the B Nutrient Application in Plant[J];Journal of Anhui Agricultural Sciences;2006-16
3 ZHANG Xue-bin et al(College of Agronomy and Biological Science and Technology,Southwest University,Chongqing 400715);Study on the Effects of Boron with Different Concentrations on Physiological and Biochemical Characteristics of Insect-resistant Cotton[J];Journal of Anhui Agricultural Sciences;2008-03
4 QI Han et al(College of Horticulture,South China University of Tropical Agriculture,Danzhou,Hainan 571737);Current Situation of the Application of Boron in the Crop Growth[J];Journal of Anhui Agricultural Sciences;2008-16
5 YANG Jia-suo et al (Zhuanglang Agriculture and Animal Husbandry Administrative Bureau,Zhuanglang, Gansu 744600);Analysis on Factors Influencing Pollen Germination and Tube Growth of Solanum tuberosum L.in vitro[J];Journal of Anhui Agricultural Sciences;2009-17
6 CHU Hai yan 1,2 ,YU Min 2, WANG Yun hua 2,WU Li shu 2,ZHU Jian guo 1 (1.LMCP,Institute of Soil Science,CAS,Nanjing 210008;2.Huazhong Agricultural University);Effects of Boron Deficiency on CAT and POD Activities of Rape (Brassica napus L.) Parents and F_1 Generations[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-03
7 HUANG Dong-feng1,WENG Bo-qi2,XIONG De-zhong3,LUO Tao1,YING Zhao-yang2 (1.Institute of Soil and Fertilizer,Fujian Academy of Agricultural Science,Fuzhou 350013,China;2.Institute of Ecological Agriculture,Fujian Academy of Agricultural Science,Fuzhou 350013,China;3.Department of Resource and Environment,Fujian University of Agriculture and Forest,Fuzhou 350002,China);Symptoms of boron stress on Chamaecrista rotundifolia and effects of applying boron fertilizer[J];Acta Prataculturae Sinica;2008-01
8 ZHAO Ling-xia', LIU Shou-wei, LI Jing-fu, XU Xiang-yang, JU Wen-huan (Northeast Agricultural University, Harbin, Heilongjiang 150030) (Tailai Agricultural Technique Extension Center Heilongjiang 162400);Study on optimization of the culture medium for Lycopersicon pollen germination[J];Journal of Northeast Agricultural University;2000-02
9 Zhang Xingwen Ren Hongyu Yan Hong (College of Resource & Environment, North East Agriculture University, Harbin, 150030);ADVANCES ON STUDY OF ABSCISSION AND ABORTION OF SOYBEAN FLOWERS AND PODS[J];Soybean Science;2002-04
10 GE Zheng-zhen1,YANG Guang-yu2,ZHONG Yan1,DU Qing-zhen1,MENG Xiang-xun1(1School of Basic Medicine and Biological Science,Soochow University,Suzhou 215123,Jiangsu;2 Soybean Research Center ,Jilin Academy of Agricultural Sciences ,Changchun 130033,Jilin,China);Effect of Boron on Cell Structure and Shape in Soybean(Glycine max) Callus[J];Soybean Science;2009-02
China Proceedings of conference Full-text Database 1 Hits
1 Xu Fangsen Wang Yunhua (Lab of Microelement, Huazhomg Agriculture University, Wuhan 430070);ADVANCE ON GENETIC DIFFERENCE OF BORON NUTRITION IN BRASSICE NAPUS[A];[C];2000
Chinese Journal Full-text Database 5 Hits
1 YI Ping WANG Li WAN Cui-Xiang ZHU Ying-Guo *(The Key Laboratory of MOE for Plant Developmental Biology and Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072, China);BAC Library Construction for Mitochondrial Genome of Sterile and Maintainer Lines from HL Rice[J];Acta Agronomica Sinica;2002-06
2 FU Bin-Ying, YANG Dai-Chang, ZHU Ying-Guo, LI Zhi-Kang ( Institute of Genetics, College of Life Sciences, Wuhan University, Wuhan 430072 China; Genome Mapping Lab., Plant Breeding, Genetics and Biochemistry Division, International Rice Research Institu;Construction of the Physical Map of Pi-2(t), a Blast Resistance Gene in Rice[J];ACTA GENETICA SINICA;2000-09
3 WANG Wen-Ming, JIANG Guang-Huai, WANG Shi-Quan, ZHU Li-Huang, ZHAI Wen-Xue (Institute of Genetics, Plant Biotechnology Laboratory, Chinese Academy of Sciences, Beijing 100101, China);Construction of A Deep Coverage Rice BAC Library and Identification of Clones Associated with Disease-resistant Genes[J];Acta Genetica Sinica;2001-02
4 QIN Rui,WEI Wen hui,NING Shun bin,JIN Wei wei,HE Guang cun,SONG Yun chun (The Centre of Developmental Biology,Wuhan University,Wuhan 430072);The Physical Location of Gm-2and Gm-6 in O.officinalis with BAC FISH Based on Comparative RFLP Map of Wild Rice, O.officinalisand Cultivated Rice,O.sativa.[J];Scientia Agricultura Sinica;2001-01
5 1PENG Kai Man 2ZHANG Hong Bin 1ZHANG Qi Fa  1(National Key Laboratory of Crop Genetic and Improvement, Huazhong Agricultural University, Wuhan 430070, China) 2(Department of Soil and Crop Sciences, Crop Bi;A BAC LIBRARY CONSTRUCTED TO THE RICE CULTIVAR “MINGHUI 63" FOR CLONING GENES OF AGRONOMIC IMPORTANCE [J];ACTA BOTANICA SINICA;1998-12
【Secondary References】
Chinese Journal Full-text Database 6 Hits
1 WAN Ya-tao1,2,FAN Chuan-zhang1,2,FENG Lu2,YOU Cheng-li2,XU Fu-rong2,TAN Xue-lin1,DAI Lu-yuan2(1.Faculty of Agrionomy and Biotechnology,Y A U,Kunming 650201,China;2.Yunnan Academy of Agricultural Sciences,Kunming 650223,China);A Survey of the Current Status of Oryza meyeriana Baill. in Yunnan Province[J];Journal of Yunnan Agricultural University;2007-02
2 WAN Ya-tao~(1,2),A Xin-xiang~2,FAN Chuan-zhang~(1,2),XU Fu-rong~2,TAN Xue-lin~1,YU Teng-qiong~2,TANG Cui-feng~2,DAI Lu-yuan~(2,*) (~1Yunnan Agricultural University,Kunming 650201,China;~2Biotechnology and Germplasm Research Institute,Yunnan Academy of Agricultural Sciences,Kunming 650223,China);ISSR Analysis on Genetic Diversity of the 34 Populations of Oryza meyeriana Distributed in Yunnan Province[J];Chinese Journal of Rice Science;2006-06
3 FU Xue-lin,LU Yong-gen,LIU Xiang-dong,LI Jin-quan(Guangdong Provincial Key Laboratory of Plant Molecular Breeding,South China Agricultural University,Guangzhou 510642,China);Progress on Transferring Elite Genes from Non-AA Genome Wild Rice into Oryza sativa Through Interspecific Hybridization[J];Chinese Journal of Rice Science;2007-06
4 Zhang Huanhuan1, Liu Rui1, Guo Haibin2, Li Yajuan2 (1College of Agriculture, South China Agricultural University, Guangzhou 510642; 2Center of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642);Advancement on Mining and Utilization of Elite Genes in Oryza officinalis Wall[J];Chinese Agricultural Science Bulletin;2009-19
5 HOU Si-ming1,2,ZHANG Wei1,3*,BAI Xian-guang1,3,ZHONG Qiao-fang1,GAO Yue-fen4,HUANG Xing-qi1,CHENG Zai-quan1* (1.Biotechnology & Genetic Germplasm Institute,Yunnan Academy of Agricultural Sciences,Yunnan Kunming 650223,China;2.College of Life Science and Technology,Kunming University,Yunnan Kunming 650221,China;3.College of Life Science,Yunnan University,Yunnan Kunming 650221,China;4.Luliang Sericulture Station,Yunnan Kunming 655600,China);Research on Efficient Extraction of Genome DNA of Oryza officinalis[J];Southwest China Journal of Agricultural Sciences;2009-05
6 DU Juan1,YANG Tao1,ZENG Ya-wen1,YANG Shu-ming1, PU Xiao-ying1,SUN Dan2,LV Hong-bin3,WANG Jiang-min4(1.Biotechnology and Genetic Resources Institute,Yunnan Academy of Agricultural Sciences,Kunming 650205,China; 2.School of Life Sciences,Yunnan University,Kunming 650091,China; 3.Institute of Food Crops,Yunnan Academy of Agricultural Sciences,Kunming 650205,China; 4.Institute of Quality Standards and Testing Technology,Yunnan Academy of Agricultural Sciences,Kunming 650223,China);A preliminary study on the functional ingredients in brown rice from different regions and types varieties[J];Journal of Yunnan University(Natural Sciences Edition);2011-02
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 Zhao Zhuqing\ Wang Yunhua\ Wu Lishu (Huazhong Agricultural University, Wuhan 430070);EFFECT OF BORON DEFICIENCY ON AUXIN METABOLISM IN CUCUMBER[J];JOURNAL OF HUAZHONG AGRICULTURAL;1998-03
2 WANG Jun Xia YANG Guang Sheng FU Ting Dong MENG Jin Ling *[WT6BX](National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan, 430070) (Department of Agronomy, Huazhong Agricultural University, Wuhan, 430070)[KH+3m;Identification of RAPD Markers Linked to the Fertility Restoring Gene for the Polima CMS in Rapeseed ( Brassica napus L.)[J];Acta Agronomica Sinica;2000-05
3 WANG Jun-Xia, YANG Guang-Sheng, FU Ting-Deng, MENG Jin-Ling (National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China) (Department of Agronomy, Huazhong agricultural University, Wuhan 430070, China);Development of PCR-based Markers Linked to the Fertility Restorer Gene for the Polima Cytoplasmic Male Sterility in Rapeseed (Brassica napus L.)[J];ACTA GENETICA SINICA;2000-11
4 Yin Jun(Shanxi Agricultural University, Taigu 030801 ),Zhu Jinyun (Shanxi Wheat Research Institute, Linfen 041000) , Paull J. G. ,Rathjen A. J. (Adelaide University,South Australia,Adelaide 5064);Effects of phosphate on wheat growth in high-boron soil.[J];;1999-03
5 Zhang Guoping (Agronomy Department, Zhejiang Agri Univ Hangzhou 310029)Ma Guorui Yang Yuai (Faculty of Enviroment and resource, Zhejiang Agri Univ Hangzhou 310029);STUDIES ON RESPONSES OF GROWTH AND FERTILITY OF DIFFERENT WHEAT GENOTYPES TO BORON LEVELS OF SOIL[J];PLANT NATRITION AND FERTILIZEN SCIENCE;1999-01
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