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《Acta Agronomica Sinica》 2004-05
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Method of Evaluation and Adjustment of Recombinant Inbred Line Population and Its Application to the Soybean RIL Population NJRIKY

WANG Yong Jun 1,2 ,WU Xiao Lei 2,YU De Yue 1,ZHANG Yuan Ming 1,CHEN Shou Yi 2,* ,GAI Jun Yi 1,* ( 1Soybean Research Institute of Nanjing Agricultural University, National Center for Soybean Improvement, National Key Laboratory for Crop Genetics and Germplasm Enhancement, Nanjing 210095, Jiangsu; 2Plant Biotechnology Laboratory, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China)  
Recombinant inbred line (RIL) population is one of the often used populations for construction of genetic map and QTL mapping There might appear some extra biased segregation in the initial RIL population due to multiple generation sampling and natural and artificial reasons Based on an analysis of the theoretical RIL population, a set of tests by using χ 2 c criteria, including test of equality, symmetry, and representativeness, was proposed For the representativeness test of the RIL population, a simulation program (GenoSim) was established in order to set up some critical values of maximum χ 2 of families and the ratio of extra biased segregation of the RIL population with respect to the number of chromosomes, markers and families Then the procedures for adjustment of the RIL population were set up This set of methods and procedures of the tests and adjustment was called Simulated Population Sampling Criteria (SPSC) By using the SPSC, a soybean RIL population NJRIKY was constructed, evaluated and adjusted The RIL population was composed of 201 F 2∶7∶9 families derived from a cross, Kefeng No 1×Nannong 1138 2 After the SPSC three tests, 17 families and five markers were deleted from the initial RIL data set to make the adjusted population fitted the equality, symmetry and representativeness criteria and therefore, the locations of the 19 markers and two linkage groups on the linkage map were changed
【Fund】: 国家 8 63项目 ( 10 1 0 2 0 2 0 3 );; 国家重点基础研究项目 (G19980 10 2 0 0 );; 南京农业大学大豆研究所与中国科学院遗传与发育生物学研究所合作项目
【CateGory Index】: S565.1
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