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《Acta Agronomica Sinica》 2008-01
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Primary Study on HMW-GS Inheritance Affected by 1BL/1RS Translocation

GENG Hui-Min1,2, ZHANG Huai-Qiong1, and REN Zheng-Long1, (1 State Key Laboratory for Plant Genetics and Breeding, Sichuan Agricultural University, Ya’an 625014, Sichuan; 2 College of Life Science, Luoyang Normal University, Luoyang 471022, Henan, China)  
The common wheat (Triticum aestivum L.) Chuannong 18 with 1BL/1RS translocation chromosome, whose HMW-GS components is (1, 7+9, 2+12) as male and female parent respectively was crossed with Chuannong 19 (N, 7+8, 2+12). Both the female and male gametes of their F2 progenies showed significantly (P0.01) lower rate of 1BL/1RS translocation compared with the theoretical value (75%), in which female gamete (65.1%) had higher rate than male gamete (52.4%). In both crosses, HMW-GS subunits (encoded by Glu-A1 and Glu-B1) of parents were all transmitted to F1 progenies as dominant trait, but devi- ated the Mendel’s segregation ratios at Glu-B1 and the combination of Glu-A1 Glu-B1 loci in F2 progenies. We also found and confirmed a variant seed in F1 of Chuannong 19 × Chuannong 18 by detecting its genome DNA with 33 pairs of specific SSR markers and the components of HMW-GS and gliadin of its F2 progeny. The HMW-GS patterns of the variant seed were (1, 7+8, x+9, 2+12). The band of variant subunit was similar to 1Bx6 by SDS-PAGE analysis, which was between 1Dx5 and 1Bx7.
【Fund】: 国家自然科学基金项目(30671136)
【CateGory Index】: S512.1
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