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Construction of A Molecular Genetic Linkage Map of Tobacco Based on SRAP and ISSR Markers

MA Hong-Bo1,QI Jian-Min1,*,LI Yan-Kun1,LIANG Jing-Xia1,WANG Tao2,LAN Tao1,CHEN Shun-Hui3,TAO Ai-Fen1,LIN Li-Hui1,and WU Jian-Mei1 (1 College of Crop or Life science,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian;2Fujian Shunchang Tobacco Company,Shun-chang 353200,Fujian;3Fujian Tobacco Company,Fuzhou 350003,Fujian,China)  
High-density genetic linkage maps are useful tools for studying genome,phylogeny and evolution,and for mapping and Cloning genes of interest.In this study,a molecular genetic map was constructed with F2 population consisting of 187 indi-viduals from a cross of flue-cured tobacco cultivar Taiyan 7 and burley tobacco cultivar Bailei 21 by using a total of 513 SRAP primers and 42 ISSR primers.Approximately 58 out of 513 and 10 out of 42 primers,respectively,showed polymorphisms.Each of this polymorphic primers produced at least one scorable polymorphic DNA band which was visible enough for detection and scoring.The map consisted of 26 linkage groups which included 112(92 SRAP and 10 ISSR) markers,and covered 1 560.2 cM with an average distance of 18.1 cM.Sixteen markers were still unlinked.All 26 linkage groups consisted of 2–20 markers rang-ing in length from 0–291.0 cM.A total of 24.1% distorted markers distributed in the map,mainly concentrated in the LG1 and LG4 linkage groups,and the others were mapped on diverse linkage groups respectively.This map will provide the basis for gene mapping and marker-assisted selection of important agronomic traits in tobacco.
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