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《Acta Agronomica Sinica》 2008-12
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Development and Utilization of EST-derived Microsatellites in Sesame(Sesamum indicum L.)

WEI Li-Bin1, ZHANG Hai-Yang2,, ZHENG Yong-Zhan2, GUO Wang-Zhen1,, and ZHANG Tian-Zhen1 (1 National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, Nanjing 210095, Jiangsu; 2 Henan Key Laboratory of Crop Improvement, Zhengzhou 450002, Henan, China)  
The deficiencies of markers in Sesamum indicum L. that can be used at home and aboard seriously restrict its studies in molecular field. To accelerate the application of molecular markers in sesame, EST-SSR markers development and utilization using publicly available sesame EST data were performed. A total of 1 785 non-redundant EST sets were assembled among the 3 328 identified sesame EST. 148 microsatellites sequences containing 155 EST-SSR were detected from these EST. The total length of the non-redundant EST sequences was 774.27 kb, on average one EST-SSR each 4.99 kb. The distribution characteris-tics of the EST-SSR markers was analyzed. Among the SSR, dinucleotide AG/TC was the most abundant (occurring 58 times), with frequency of 37.42%. According to these EST sequences containing SSR, 50 primer pairs were designed and tested on 36 sesame accessions, 2 cotton accessions, 2 soybean accessions, and 2 oil sunflower accessions to detect polymorphisms and trans-ferability. With 44 EST-SSR, 108 loci were successfully amplified in sesame, with an average of 2.45 loci per primer pair. Of the 44 amplified primer pairs, 27(61.4%) primer pairs revealed polymorphisms in the 36 sesame accessions. The PIC (polymorphism information content) ranged from 0.105 to 0.844, with an average of 0.390. Based on genetic similarity coefficient, the UPGMA dendrogram grouped 26 of 36 accessions in to two sub-clusters (III and IV), but it revealed no association between genotypes and geographical sources. In addition, 2, 3, and 4 SSR markers could be transferred to the PCR of cotton, soybean and oil sunflower respectively. This study effectively proved that EST-SSR from sesame is valuable for genetic analysis, linkage mapping and transferability study among oil plants.
【Fund】: 国家自然科学基金项目(30471104);; 国家重大基础研究前期研究专项(2003CCAO0700);; 教育部111工程项目(B08025)
【CateGory Index】: S565.3
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