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《Acta Agronomica Sinica》 2013-06
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Genetic Evolution for Vigna Subgenus Ceratotropis Based on Chloroplast DNA Sequences

LIU Yan, CHENG Xu-Zhen , WANG Li-Xia, WANG Su-Hua, and BAI Peng Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China  
China has abundant germplasm resources of subgenus Ceratotropis, but the related study of species within this subgenus is much lagged. In order to investigate genetic relationship and evolution process among different species, 110 entries of Ceratotropis were analyzed by using intron rpl16, rps16 and intergenic regions psbA-trnH, rpoB-trnC, and trnL-trnF of chloroplast genome. The results revealed that parsiminfo site percentage was from 3.63% to 24.28%, with the highest for rps16 and the lowest for rpoBtrnC. A higher level conservation of sequence was observed within species than interspecies. Kimura-2-parameter distance was between 0 and 0.057 calculated based on five chloroplastassembling sequences. V. minima complex (including V. minima, V. riukinensis, and V. nakashime) and V. subramaniana had the longest distance. Phylogenetic trees constructed by neighborjoining (NJ) and maximum parsimony (MP) methods in MEGA 5.1 and PAUP 4.0 revealed that eighteen species and subspecies were divided into two branches. The groupIcontained nine species and subspecies grouped into three sections complex, including Angulares, Ceratotropis and Aconitifoliae. The group II contained nine species and subspecies which completely belonged to section Angulares. Partition homogeneity test of phylogenetic trees of the five sequences showed that their topology was highly consistent (P=0.87). The present study provides the important information for clarifying genetic relationship among differ- ent species within Ceratotropis.
【Fund】: 北京市自然科学基金项目(6103025);; 国家现代农业产业技术体系建设专项(CARS-09);; 国家公益性行业(农业)科研专项(nyhyzx07-017)资助
【CateGory Index】: S643.1
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