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《Journal of Chinese Integrative Medicine》 2010-06
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Optimal combination of baicalin,icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne

Wei-yi GONG1,Xin-min ZHANG1,Zi-yin SHEN1,Guo-rang HU2,Su-qin ZHANG1,Run-hong LIU1,Wei-dong ZHANG3,Jing-cheng DONG11.Institute of Chinese Integrative Medicine,Huashan Hospital,Fudan University,Shanghai 200040,China 2.Department of Immunology and Allergy,Concord Hospital,Sydney University,Sydney 2006,Australia 3.School of Pharmacy,Second Military Medical University,Shanghai 200433,China  
Objective:To study the optimal combined ratio of baicalin,icariin and Astragalus saponin Ⅰ from a Chinese herbal compound Biminne.Methods:Firstly,a mouse model of allergic rhinitis was established by intraperitoneal injection of ovalbumin (OVA) and aluminum hydroxide gel suspension,and the effective dose range of baicalin,icariin and Astragalus saponin Ⅰ was detected by 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide inner salt method.Secondly,10 groups of combinations of baicalin,icariin and Astragalus saponin Ⅰ assembled by U10(108) form were employed to determine the optimal combination by means of analyzing of the inhibitory effect on the splenocyte proliferation.Finally,the effects of each effective ingredient and the optimal combination were compared by observing the splenocyte proliferation,the contents of interleukin-4 (IL-4),IL-5,interferon-gamma (IFN-γ) in supernatant of the splenocyte cultures and the ratio of IL-4 to IFN-γ in order to verify the result.Results:Baicalin or icariin at concentrations ranging from 2 to 10 μmol/L,and Astragalus saponin Ⅰ from 1 to 10 μmol/L effectively suppressed the splenocyte proliferation.When the proportion of baicalin,icariin and Astragalus saponin Ⅰ was 1:2.14:2.65,the inhibitory effect was most remarkable.Further research confirmed the rationality of the optimal combination.Conclusion:An optimal combination of the major effective ingredients from Chinese herbal compound Biminne most effectively suppresses the proliferation of splenocytes from sensitized mice and regulates the cytokine secreting.
【Key Words】： Chinese herbal compound allergic rhinitis cell proliferation compatibility uniform design lymphocytes
【Fund】： 上海市科学技术委员会攻关项目(No.02DZ19128)
【CateGory Index】： R289
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