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《Chinese Journal of Coll Biology》 1995-04
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A STUDY OF MEGAKARYOCYTES DIFFERENTIATED FROM MURINE HIGH PROLIFERATIVE-POTENTIAL COLONY-FORMING CELLS (HPP-CFC) IN VITRO

ZHANG Xue Jun JIANG Li Zhen WANG Xin Ming, HAN Zhong Chao (Shanghai Institute of Cell Biology, Academia Sinica, Shanghai 200031)  
In the present study, a new subset of HPP-CFC denominated high-proliferative-potential mixed colony-forming unit-megakaryocyte, (HPP-mCFU-MK), was detected in normal and 5-fluorouracil (5-FU) treated murine bone marrow cells in plasma clot cultures. The HPP-mCFU-MK was not only able to form macroscopic colonies that fit the criteria of the HPP-CFC colony, but also tc grow a number of megakaryocytes. Their growth was dependent on complementing either aplastic anemia serum (AAS) or a combination of three or more recombinant he-matopoietic growth factors, but it was ineffective for adding inhibitory factors, transforming growth factor β1 (TGF-β1) and platelet factor 4 (PF4), in vitro. The recoloning of the 12-day-old HPP-mCFU-MK colonies picked up from AAS-stimulated primary cultures caused a secondary formation of HPP-CFC colonies. These results strongly suggest that HPP-mCFU-MK is a new subset of stem cells in murine bone marrow.
【CateGory Index】: Q25
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【References】
Chinese Journal Full-text Database 1 Hits
1 Xia Tian Jiang Dezhao(Research Laboratory of Blood Physiology, Hunan Medical University, Changsha 410078);Culture of murine HPP-CFC in vitro using conditioned mediumsubstitute for recombinant growth factors[J];BULLETIN OF HUNAN MEDICAL UNIVERSITY;1998-02
【Co-references】
Chinese Journal Full-text Database 1 Hits
1 WANG Cheng-Long LI Wei-Min WANG Qi-Ru (Experimental Hemophysiology Laboratory, Hunan Medical University Changsha 410078);BIOLOGICAL CHARACTERISTICS OF L929 CELL LINE[J];Journal of Experimental Hematology;1995-04
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