Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Cell Biology》 2008-01
Add to Favorite Get Latest Update

Effects of Quercetin on Growth of Colon Cancer Cell Line RKO

Sai-Nan Tan1,2, Cai-Ling Lu2, Yi Cui2, Xu Ma2 (1Graduate School of Peking Union Medical College, Beijing 100730, China; 2Department of Genetics, National Research Institute for Family Planning, Beijing 100081, China)  
MTT assay tested cell growth of colon cancer cell line RKO; cell cycle and cell apoptosis was observed by flow cytometry technique; RT-PCR and Western blot technique detected the expression of p21, p27 and p53. MTT assay showed that quercetin significantly inhibited cell growth and had dose-dependent effect; Flow cytometry technique showed that with 5 μmol/L quercetin the cell cycle was arrested in G0/G1 phase; apoptosis rates of 5, 10, 20 μmol/L groups were 23.4%, 24.2%, 47.9%, and apoptosis rate of the control group was 13.2%; mRNA and protein expression level of p21 and p27 increased, the expression level of pro-apoptotic protein p53 increased. Quercetin has significant growth inhibition on RKO cells. Cell cycle is arrested in G0/G1 phase by upregulating the expression of p21 and p27; quercetin may also induce apoptosis by upregulating the expression of p53.
【Fund】: 国家重点基础研究发展计划(973计划)资助(No.2007CB5119005)~~
【CateGory Index】: R285.5;R735.35
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved