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《Acupuncture Research》 2012-04
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Effects of Electroacupuncture and Manual Acupuncture Interventions on Contents of Serum Inflammatory Cytokines in Rats with Chronic Adjuvant Arthritis

LI Wen-xun,HUANG Yi-ran,LEI Lin-dan,LI Na(School of Acu-moxibustion and Massage,Beijing University of Chinese Medicine,Beijing 100029,China)  
Objective To observe the effect of electroacupuncture(EA) or manual acupuncture of unilateral or bilateral "Zusanli"(ST 36) on the contents of serum inflammatory factors interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α),and anti-inflammatory factors interleukin 2(IL-2),interleukin 4(IL-4) and interleukin 10(IL-10),so as to reveal their mechanism underlying improvement of chronic arthritis.Methods Sixty SD rats were randomly divided into normal control,model,electroacupuncture(EA)-left(L) Zusanli(ST 36),EA-bilateral(Bi)-ST 36,and manual acupuncture(MA)-L-ST 36 groups(12 rats/group).Arthritis model was established by subcutaneous injection of complete Freund's adjuvant(0.1 mL)into the hindpaw.EA(2 Hz/100 Hz,1-3 mA,pulse duration: 0.6 ms/0.2 ms) or manual needle stimulation was applied to the left or bilateral "Zusanli"(ST 36) for 30 min,twice every week for 4 weeks.Enzyme linked immunosorbent assay(ELISA) was used to detect the contents of serum IL-1β,TNF-α,IL-2,IL-4 and IL-10,respectively.Results Compared with the normal control group,the contents of serum inflammatory factors IL-1β and TNF-α,anti-inflammatory factors IL-4 and IL-10 were increased significantly in the model group(P0.001).In comparison with the model group,serum IL-1β,TNF-α,IL-4 and IL-10 contents were down-regulated considerably in the EA-L-ST 36,EA-Bi-ST 36 and MA-L-ST 36 groups(P0.001,P0.005).Serum IL-2 content had no apparent changes after modeling in comparison with the normal group(P0.05).While serum IL-2 contents were markedly higher in the EA-L-ST 36,EA-Bi-ST 36 and MA-L-ST 36 groups than in the model group(P0.05).No significant differences were found among the EA-L-ST 36,EA-Bi-ST 36 and MA-L-ST 36 groups in serum IL-1β,TNF-α,IL-2,IL-4 and IL-10 contents(P0.05).Conclusion EA or manual acupuncture stimulation of single or bilateral ST 36 can effectively down-regulate serum inflammatory factors IL-1β and TNF-α levels,and upregulate anti-inflammatory factor IL-2 level in chronic arthritis rats,which may contribute to their effects in relieving chronic arthritis.
【CateGory Index】: R245
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