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《Modern Chemical Industry》 2008-S2
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Study on fusion of biparental inactivated protoplasts of Streptomyces gilvosporeus

LUO Jian-mei,LI Jian-shu,WANG Yan-ting,WANG Min(College of Biotechnology,Tianjin University of Science and Technology,Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China)  
The important factors including biparental inactivation,PEG relative molecular weight,PEG concentration,the fusion time,the fusion temperature,the concentration of inorganic ion are studied for protoplast fusion of S.gilvosporeus SG-2002-1 and S.gilvosporeus SG-2002-2.Protoplasts are completely inactivated by UV for 2 min and heat for 1 h at 60℃,respectively.The optimum conditions of protoplast fusion are as follows:PEG relative molecular weight of 6 000,PEG concentration of 50%,the fusion time of 8 min,the fusion temperature of 25℃,the concentration of CaCl2 0.05 mol/L,the concentration of MgCl2 0.02 mol/L.Under the above optimized conditions,the fusion rate reached 5.0%.
【Fund】: 天津市应用基础研究计划面上项目(07JCYBJC07800);; IFS国际科学基金(F/4239-1);; 天津科技大学引进人才科研启动基金(20060426)
【CateGory Index】: Q93;Q78
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