Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Modern Chemical Industry》 2008-S2
Add to Favorite Get Latest Update

Study on fusion of biparental inactivated protoplasts of Streptomyces gilvosporeus

LUO Jian-mei,LI Jian-shu,WANG Yan-ting,WANG Min(College of Biotechnology,Tianjin University of Science and Technology,Tianjin Key Laboratory of Industrial Microbiology,Tianjin 300457,China)  
The important factors including biparental inactivation,PEG relative molecular weight,PEG concentration,the fusion time,the fusion temperature,the concentration of inorganic ion are studied for protoplast fusion of S.gilvosporeus SG-2002-1 and S.gilvosporeus SG-2002-2.Protoplasts are completely inactivated by UV for 2 min and heat for 1 h at 60℃,respectively.The optimum conditions of protoplast fusion are as follows:PEG relative molecular weight of 6 000,PEG concentration of 50%,the fusion time of 8 min,the fusion temperature of 25℃,the concentration of CaCl2 0.05 mol/L,the concentration of MgCl2 0.02 mol/L.Under the above optimized conditions,the fusion rate reached 5.0%.
【Fund】: 天津市应用基础研究计划面上项目(07JCYBJC07800);; IFS国际科学基金(F/4239-1);; 天津科技大学引进人才科研启动基金(20060426)
【CateGory Index】: Q93;Q78
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 MAO Yu 1 WANG Dan 2,3 HUANG Zhan-bin 1 XING Jian-min 2 (1 School of Chemical and Evironmental Engineering,China University of Mining&Technology,Beijing 100083,China) (2 National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China) (3 Graduate University of Chinese Academy of Sciences,Beijing 100049,China);Application of Microbial Protoplast Fusion Technology in Genetic Breeding[J];China Biotechnology;2010-01
Chinese Journal Full-text Database 1 Hits
1 ZHENG Zhong-yi~1,XIE Da-ping~1,TAN Zhou-jin~2,XIAO Ke-yu~2,LI Yu-hong~1 (1.College of BioScience and Technology,Hunan Agricultural University,Changsha 410128,PRC;2.Hunan Agricultural University,Changsha 410128,PRC);Influence Factors of Microbial Protoplast Fusion Technology[J];Hunan Agricultural Sciences;2006-04
Chinese Journal Full-text Database 10 Hits
1 LUO Ying-lan,LI Yuan-wen,XIA Zhi-lan(Edible Fungi Institute,Hunan Agricultural University,Changsha 410128,PRC);Research on the Electrofusion Conditions of Protoplasts Between 'Jigu 5' and 'Shuyou1'[J];Hunan Agricultural Sciences;2008-01
2 CHENG Kun,YU Tong-li,SUN Hai-yun(Food and Bioengineering Department,Shandong Light Industry Institute,Jinan,Shandong,250100);Study and application of Natamycin[J];Jiangsu Condiment and Subsidiary Food;2006-01
3 Wang Yong,Ma Yue,Zhao Qin,Wang Qiuju,Wan Xiang (Veterinary Department,Southwest University,Chongqing 402460);Generation of Riemerella anatipestifer protoplast[J];Modern Journal of Animal Husbandry and Veterinary Medicine;2009-01
4 CUI Jun-tao1, ZHANG Wei2, CAO Tian-shu1 (1.College of Resource and Environment Science, Jilin Agriculture University, Changchun 130118, China; 2.Jilin Agriculture School, Changchun 130031, China);Preparation and Selection of Atrazine Degradation Fusants[J];Journal of Agro-Environment Science;2008-06
5 CHENG Kun and YU Tong-li (Department of Food and Bioengineering, Shandong Institute of Light Industry ,Ji'nan, Shandong 250353,China);Study on the Inhibition of Natamycin on the Growth of Saccharomyces cerevisiaes & Its Application[J];Liquor-Making Science & Technology;2007-03
6 Xu Jinhui1,2,Liu Shuwen1,Dong Xinping2(1.The Wine Institute of The Northwest Agricultural and Forestry University,Yangling,Shanxi 712100;2.Suntime International Wine Co.,Ltd.Manas County,Xinjiang 832200);Study on the Inhibition of Natamycin on the Growth of Wine Yeast[J];Sino-Overseas Grapevine & Wine;2008-05
7 Zhou Li (College of Food Science,Southwest University,ChongQing 400716,China);Application of Natamycin in Meat Products as the Preservative[J];Meat Research;2008-08
8 ZHOU Li (College of Food Science, Southwest University, ChongQing 400716, China);The Crude Preservative——Natamycin[J];Meat Research;2008-12
9 MENG Xian-jun,ZHENG Feng-e,ZHANG Qi(Food Science Department of Shenyang Agricultural College,Shenyang 110161,China);Study on the mutagenic breeding of the strain of Streptomyces natalensis by electrofusion impulse[J];Science and Technology of Food Industry;2008-03
10 HUANG Kai1,YUAN De-bao2,HAN Zhong2(1.Guangxi Vocational and Technical Institute of Industry,Nanning 530001,China;2.School of Light Industry and Food Science,South China University of Technology,Guangzhou 510640,China);Isolation,identification and antimicrobial effect in vitro of causative pathogen from mouldy fresh walnut during storage[J];Science and Technology of Food Industry;2009-01
China Proceedings of conference Full-text Database 2 Hits
1 SHAO Jin-liang; YUAN Wei; DONG Wen-ming; JIAO Ling-mei ( College of Food Science and Technology Yunnan Agricultural University, Kunming 650201; Nurse Department of Hainan Medical College, Haikou 571101);Research the properties and application in the food industry of bio-preservatives natamycin[A];[C];2006
2 Li Haibo Cui Wenjing Cheng Shumei Ma Wen Li Yingjun Zhang Wei (College of Food Science and Technology,Agricultural University of Hebei,Baoding,Hebei 071001,China);Natamycin production strains of protoplast mutagenesis and breeding bacteria[A];Chinese Society of Agricultural Engineering[C];2007
Chinese Journal Full-text Database 10 Hits
1 ZHU He-shui et al(College of Animal Husbandry and Veterinary,Henan Agricultural University,Zhengzhou,Henan 450002);Application of Protoplast Fusion Technology[J];Journal of Anhui Agricultural Sciences;2007-05
2 LI Xiang-kai,AN Zhi-dong, ZHU Fei (College of Life Sciences, Wuhan University, Wuhan 430072);Study on Fusion of Biparental Inactivated Protoplasts of Streptomyces lincolnensis[J];Amino Acids & Biotic Resources;2001-04
3 WANG Wan-neng,QUAN Xue-jun, ZHOU Yue-gang,HU Yong (College of Bioengineering, Chongqing Institute of Technology, Chongqing400050,China);Research on the Biological Control of Plant Diseases[J];Journal of Chongqing Institute of Technology;2004-02
4 WEI Yan min, LIU Da qun, TIAN Shi min, ZHANG Ting (Department of Plant Pathology, Agricultural University of Hebei, Baoding 071001, China)[HJ4/9];The antagonism of 26 strains of Streptomyces spp. against several vegetable pathogens[J];JOURNAL OF AGRICULTURAL UNIVERSITY OF HEBEI;2000-03
5 DONG Li, MENG Qing fang, LIU Da qun (College of Plant Protection, Agricultural University of Hebei, Baoding 071001, China);Advances on breeding technology of high-yield strains in agricultural antibiotic[J];Journal of Agricultural University of Hebei;2002-S1
6 LIU Qiu~(1,2) YU Ji-cheng~1 YAN Jian-fang~1 FAN Sheng-di~1(1.College of Life Science,Dalian Nationalities University,Dalian Liaoning 116605,China;2.Plant Protection College of Shenyang Agricultural University,Shenyang 110161,China);Protoplast Fusion of Streptomyces Chromogenes SY20-2 and Streptomyces Rimosus SY20-4[J];Journal of Dalian Nationalities University;2006-05
7 SHU Xiu-lin,AN De-rong est Management Ministry of Education,The People's Republic of China,Northwest Sci-Tech University of Agriculture and Forestry,Yangling,Shaanxi 712100,China);Studies on the effect of zuelaemycin producing actinomycetes strain S-5120 on downy mildew of cucumber[J];Acta Botanica Boreali-occidentalia Sinica;2004-11
8 CHEN Guang rong,XIAO Ke yu,WENG bo,ZHONG Lei,DENG Shi ming,HE Yan lin (College of Animal Science and Technology,Hunan Agricultural University,Changsha,Hunan,410128,China);Application of Cell Fusion Technique in Biological Medicine[J];Progress In Veterinary Medicine;2004-01
9 WANG Chun-ping1,2,WEI Qiang1,BAO Guo-lian1,LIU Yan1,SHAO Ze-xiang1,2,JI Quan-an1(1.Animal Husbandry and Veterinary Institute,Zhejiang Academy of Agricultural Sciences,Hangzhou,Zhejiang,310021,China;2.College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai'an,Shandong,271018,China);Progress on Microbial Protoplast Fusion Technology[J];Progress in Veterinary Medicine;2008-05
10 CHENG Ji1, HONG Wen-rong1, SU Jian-zhang1, YE Li-rong2 (1. College of Qiaoxing Light Industry, Fuzhou University, Fuzhou, Fujian 350002, China; 2. Fuzhou Antibiotic General Factory, Fuzhou, Fujian 350002, China);Study on protoplast fusion between Streptomyces tenebrarius and Streptomyces kanamyceticus[J];Journal of Fuzhou University(Natural Sciences Edtion);2003-01
China Proceedings of conference Full-text Database 3 Hits
1 ;Promote the development of Chinese-charatered papermaking industry by utilizing non-wood fibre in reasonable ways[A];[C];2005
2 MA Yongsheng, QIU Huayu, WANG Shaoqiang WANG Caiyou (Shandong Key Laboratory of Pulp and Paper Engineering, Shandong Institute of Light industry Anqiu KEYANG Machine Co. Ltd. );Study of Chitosan -cation starch graft copolymer as Paper Strengthening Agent[A];[C];2005
3 Shandong Institute of Light Industry Fu Lihong Qi Yongqi;Development of Studies on the Applicable of Collagen in Papermaking[A];[C];2005
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 GU Ke-dong (Medical College, Northwest Minority Universtiy, Lanzhou 730000, China);THE SCREENING OF LIPASE-PRODUCING STRAIN AND THE PREPARATION OF ITS PROTOPLAST[J];Journal of Gansu Sciences;2003-04
2 CHEN Jiang-yuan~1, DAI Jiang-hong~2, LIU Zhi-guo~2, ZHAO Jing-guo~2,WANG Ya-lin~2,QU Shen~3(1.Wuhan Nursing School, Wuhan 430040,China;2.Department of Biotechnology and Chemical Engineering, Wuhan Polytechnic University, Wuhan 430023, China; 3.Department of Biochemistry & Molecular Biology, Huazhong University of Science and Technology, Wuhan 430022,China);Strain Improvement of Lysergic Acid Amid Producing Claviceps Paspali by Protoplast Mutagenesis[J];;2005-07
3 Ren Tao Huang Qinyun Ou Shoushu (Dept. Of Veterinary Medicine, South China Agric.Univ.,Guangzhou,510642);STUDIES ON SPHEROPLAST FORMATION AND REGENERATION OF Esherichia coli O 2(Nor r,Chl s) AND O 78 (Chl r,Nor s) STRAINS[J];JOURNAL OF SOUTH CHINA AGRICULTURAL UNIVERSITY;1998-01
4 TAN Zhou-jin+1 YANG Hai-jun+2 XIAO Qi-ming+2 LIN Shu+3 WU Tie+3=(1.College of Bio-safety Science and Technology, Hunan Agricultural University, Changsha, Hunan, 410128; 2.College of Plant Protection, Hunan Agricultural University, Changsha, Hunan,410128; 3.College of Animal Science, Hunan Agricultural University,Changsha,Hunan,410128 );PROTOPLAST FUSION TECHNOLOGY AND MICROBIAL BREEDING[J];Acta Agriculturae Nucleatae Sinica;2005-01
5 CUI Zong-Qiang LUO Xin-Chang (Institute of Applied Mycology, Huazhong Agricultural University, Wuhan, 430070; Key Laboratory of Fermentation,Yunnan University,Kunming,650091);PROTOPLAST ISOLATION AND REGENERATION OF MORCHELLA[J];Mycosystema;2003-03
6 SONG Ai-Huan LI Hong-Ye** LIU Xiao-Hong( College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China);Protoplast Isolation and Regeneration in Penicillium digitatum[J];Journal of Agricultural Biotechnology;2004-02
7 ZHANG Ji hui, GUO Shun xing, WANG Chun lan, XIAO Pei gen (Institute of Medicinal Plants ,Chinese Academy of Medical Science,PUMC, Beijing 100094, China);Study on the protoplast preparation and regeneration of Gliocladium sp. producing antiinflammation compound 6,22-diene-5,8-epidioxy ergosta-3-hydroxy[J];Chinese Journal of Biochemical Pharmaceutics;2001-02
8 CHEN Min1,2,JIANG Yu-jian2,YAO Shan-jing1,LU Yi1,ZHU Lin-hua2 (1.Department of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China; 2.Department of Biotechnology Engineering, Zhejiang Gongshang University, Hangzhou 310035, China);Study on the Preparation of Pleurotus eryngii Protoplast[J];Food Science;2005-08
9 WANG Li-juan, ZHANG Peng-pai, YANG Sheng-yu(College of Life Science, Henan University, Kaifeng 475001);Conditions about protoplast preparation of nocardia.sp HD9611[J];Food Science and Technology;2005-02
10 CHEN Chun-tao,WANG Sui-lou,WEI Jun(Dept.of Food and Biology Engneering,Zhengzhou Light Industry College,Zhengzhou 450002);Protoplast formation,regeneration and mutagenization ofred yeast producting carotenoids[J];Biotechnology;2002-03
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved