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《Chinese Journal of Clinical Rehabilitation》 2003-27
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Relationship between the recovery of immunological function and interleukin-10 gene transfection

Wei Tang,Chao Liu,Yue Jia,Cui-Ping Liu,You-Wen Qin,Guo-Liang Lü,Xin Wang,M in Sun,Xiao-Dong Mao,Department of Endocrinology, First Affiliated Hospital,N anjing Medical University,Nanjing 210029,Jiangsu Provine,China Dong-Ping Guo☆,Department of Pathophysiology,Nanjing Medical University,Nanj ing 210029,Jiangsu Province,China  
AIM:To explore the effect of gene transfection on redressing the immunological functional abnormality of experimental autoimmune thyroiditis(EAT) rats on the basis of establishing EAT animal models. METHODS:Wistar female rats were selected to establish EAT animal models.The ra ts were divided into four groups:group A(PBS+PLL,n=5),group B(pORF+PLL,n=5),g roup C[pORFm interleukin(IL)-10 +PLL,n=10], group D(pORFmIL10+MEM,n=5).The me thod of radio-immunity was used to determine the titre of TgAb and that of Char reive to calculate the infiltrative index of thyroid endolymphatic cell.Spleen l ymphocytes were cultured,and -TdR marking method was used to determine the value of cpm and calculate the proliferation index. RESULTS:The titre of TgAb and TmAb in group C at the 4,6,8 weeks decreased sig nificantly than those in group A,B and D(P 0.001,F=42.66,32.65,9.66,22.25,81.3 5,14.84). The infiltrative index of thyroid endolymphatic cell in group C was ( 1.10±0.18), which was significantly lower than those in other 3 groups(P 0.01 ,F=4.39).Besides,the result of lymphocytes proliferation index test showed that proliferation index of the cells,which were stimulated by PTg,in group C were s ignificantly lower than those in group A and B(P 0.05,F=2.78).
【Fund】: 江苏省科技青年基金(BQ2000017)~~
【CateGory Index】: R581
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