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《Chinese Journal of Clinical Rehabilitation》 2006-03
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Protective effects of salvia miltiorrhizae on oxidative stress in rats with focal cerebral ischemia

Liu Chang1, Min Lian-qiu1, Ji Zhan-sheng1, Wang Qian1, Jia Yu-jie1, Li Shu-yun21Department of Neurology, First Affiliated Hospital, Jinzhou Medical College, Jinzhou 121000, Liaoning Province, China; 2Functional Central Laboratory, Jinzhou Medical College, Jinzhou 121000, Liaoning Province, China  
AIM: To investigate the protective effects of salvia miltiorrhizae on focal cerebral ischemia and oxidative stress in rats and its optimal dose. METHODS: The experiment was completed in Scientific Research Center of Jinzhou Medical College from October 5 to November 20, 2004. Sixty healthy SD rats were randomly divided into the sham-operative group, focal cerebral ischemia group and salvia miltiorrhizae groups of 1.5 g/kg, 4.5 g/kg and 13.5 g/kg with 12 in each group. Embolization thread was inserted into all the rats except those in the normal group for the establishment of model of middle cerebral artery occlusion. Three days and 30 minutes before MCAO, the rats in salvia-treatment groups were intra-abdominally injected with 1.5 g/kg, 4.5 g/kg and 13.5 g/kg of salvia miltiorrhizae. The neurological function was observed by a method of grading on a scale of 0-4 and the activity of superoxide dismutase (SOD) and contents of malondialdehyde (MDA) were measured after 24 hours' focal cerebral ischemia, as well as the histopathological changes of cerebral tissues. RESULTS: By supplement, 60 rats were involved in the result analysis. ① Neurological deficit scores:Compared with that of sham-operative group, the scores of ischemia group evidently increased; while the treatment with salvia miltiorrhizae (1.5 g/kg, 4.5 g/kg and 13.5 g/kg) significantly decreased neurological deficit scores (1.67±0.65), (1.58±0.52), (1.50±0.80), (2.50±0.80), P 0.05, especially for the dose of 1.5 g/kg (P 0.01). ② Histopathological changes: The result showed that salvia could protect against cellular edema in hippocampus. Moreover, compared with ischemia group, the number of survival neurons in the CA1 sectors of the hippocampus of salvia-treatment groups increased evidently (75.3±4.1)/HP, (76.3 ±5.0)/HP, (82.5±7.1)/HP, (62.8±6.8)/HP, P 0.01. ③ The salvia groups (1.5 g/kg, 4.5 g/kg and 13.5 g/kg) could respectively increase the activity of T-SOD, which were evidently differential with model groups (55.32 ±6.39), (50.55±5.87), (51.44±7.06), (39.28±5.67), P 0.01. However, the salvia miltiorrhizae group of 1.5 g/kg was evidently different from those of 4.5 g/kg and 13.5 g/kg at the activity of Mn-SOD (33.90±5.10), (34.13 ±3.85), (42.49±2.61) U/mg, P 0.05. ④ The contents of MDA in salvia miltiorrhizae groups were observably reduced as compared with those in model group (11.13±5.49), (11.73±6.87), (21.54±10.50) μmol/g, P 0.05, especially for the dosage of 1.5 g/kg (9.96±4.08) μmol/g, P 0.01. CONCLUSION: Salvia miltiorrhizae can reduce the product of free radical, protect nerve cell against oxidizing injury, especially the protection of low dosage. The mechanism is possiblly related with increasing activity of Mn-SOD.
【Fund】: 辽宁省教育委员会资助(991721156)~~
【CateGory Index】: R285.5
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