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《Chinese Journal of Clinical Rehabilitation》 2006-20
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Expressions of intercellular adhesion molecule and matrix metalloproteinase 2 in lung tissues of patients with chronic obstructive pulmonary diseases

Kong Ying-jun, Shi Yu-zhi, Zhang Yi-mei, Huo Jian-min Department of Respiratory Medicine, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China  
AIM: To evaluate the distribution, expression and interaction of matrix metalloproteinase 2(MMP-2), intercellular adhesion molecule-1 (ICAM-1) protein and mRNA in the lung tissues of patients with chronic obstructive pulmonary diseases (COPD) and its correlation with airflow obstruction. METHODS: Totally 28 edge lung tissue samples (the distance of tumor tissue 5 cm) were selected from the chest surgical excisions of lung tumor patients at the First Hospital and Third Hospital, Affiliated to Harbin Medical University. According to the smoking history, COPD history, X ray sheet of chest and pulmonary function, all the patients were divided into two groups: Control group (n=9 without smoke or COPD history) and COPD group (n=19 with smoking and COPD history). The forced expiratory volume in first second (FEV1) percentage, forced vital capacity (FVC) percentage and FEV1/FVC percentage were detected and taken as the pulmonary function change index. The expressions of MMP-2, ICAM-1 protein and mRNA in lung tissues were detected by immunohistochemistry method and reverse transcription-polymerase chain reaction (RT-PCR), and the correlative analysis was conducted. RESULTS: Totally 28 patients were involved in the result analysis.1The change index of pulmonary function: Difference was significant between control group and COPD group in FEV1 percentage, FVC percentage, and FEV1/FVC percentage (P 0.01). 2Immunohistochemistry staining result: MMP-2 was highly expressed in alveolar epithelial cells, bronchial epithelial cells, vascular smooth muscle cells, alveolar macrophages and interstitial cells in COPD group, compared with the control group(2.47±0.70), (1.22±0.44), with the significant difference(P 0.01); The expression of ICAM-1 was high in alveolar epithelial cells in COPD group, compared with the control group(2.58±0.51),(0.67±0.87), with the significant difference(P 0.01).③RT-PCR result: The average absorbance (A) value of MMP-2 semi-quantitative analysis was higher in CDPD group than in the control group, with the significant difference (0.54±0.14),(0.19±0.08),P 0.01.Similarly, ICAM-1 expression was significantly higher in the COPD group compared with controls(0.62±0.15),(0.37±0.11), P 0.01.④The correlative analysis: Both mRNA and protein levels of MMP-2 were inversely correlated with FEV1 percentage and FEV1/FVC percentage respectively (r=-0.577,-0.768,-0.531,-0.591,P 0.05); Similarly, a inversely correlation was also found between ICAM-1 expression (mRNA and protein) and FEV1 percentage and FEV1/FVC percentage (r=-0.584, -0.529,-0.580,-0.721,P 0.01). The expression of MMP-2 and ICAM-1 was positively correlated both on mRNA (r=0.535, P 0.01) and protein levels (r=0.664, P 0.01). CONCLUSION: Over-expressions of MMP-2 in lung tissues of patients with CDPD may lead to the abnormal support action of airway structural cells, and improve the chemotaxis and migration of inflammatory cells through ICAM-1, release certain inflammatory factors, move into the basement membrane and airway epithelial cells. In addition, highly expressed MMP-2 may participate in the inflammatory reaction of airway wall and result in the reconstitution of the lung tissues, which causes and aggravates the airway obstruction in patients with CDPD.
【CateGory Index】: R563.9
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