Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Chinese Journal of Clinical Rehabilitation》 2006-20
Add to Favorite Get Latest Update

Expressions of intercellular adhesion molecule and matrix metalloproteinase 2 in lung tissues of patients with chronic obstructive pulmonary diseases

Kong Ying-jun, Shi Yu-zhi, Zhang Yi-mei, Huo Jian-min Department of Respiratory Medicine, First Affiliated Hospital of Harbin Medical University, Harbin 150001, Heilongjiang Province, China  
AIM: To evaluate the distribution, expression and interaction of matrix metalloproteinase 2(MMP-2), intercellular adhesion molecule-1 (ICAM-1) protein and mRNA in the lung tissues of patients with chronic obstructive pulmonary diseases (COPD) and its correlation with airflow obstruction. METHODS: Totally 28 edge lung tissue samples (the distance of tumor tissue 5 cm) were selected from the chest surgical excisions of lung tumor patients at the First Hospital and Third Hospital, Affiliated to Harbin Medical University. According to the smoking history, COPD history, X ray sheet of chest and pulmonary function, all the patients were divided into two groups: Control group (n=9 without smoke or COPD history) and COPD group (n=19 with smoking and COPD history). The forced expiratory volume in first second (FEV1) percentage, forced vital capacity (FVC) percentage and FEV1/FVC percentage were detected and taken as the pulmonary function change index. The expressions of MMP-2, ICAM-1 protein and mRNA in lung tissues were detected by immunohistochemistry method and reverse transcription-polymerase chain reaction (RT-PCR), and the correlative analysis was conducted. RESULTS: Totally 28 patients were involved in the result analysis.1The change index of pulmonary function: Difference was significant between control group and COPD group in FEV1 percentage, FVC percentage, and FEV1/FVC percentage (P 0.01). 2Immunohistochemistry staining result: MMP-2 was highly expressed in alveolar epithelial cells, bronchial epithelial cells, vascular smooth muscle cells, alveolar macrophages and interstitial cells in COPD group, compared with the control group(2.47±0.70), (1.22±0.44), with the significant difference(P 0.01); The expression of ICAM-1 was high in alveolar epithelial cells in COPD group, compared with the control group(2.58±0.51),(0.67±0.87), with the significant difference(P 0.01).③RT-PCR result: The average absorbance (A) value of MMP-2 semi-quantitative analysis was higher in CDPD group than in the control group, with the significant difference (0.54±0.14),(0.19±0.08),P 0.01.Similarly, ICAM-1 expression was significantly higher in the COPD group compared with controls(0.62±0.15),(0.37±0.11), P 0.01.④The correlative analysis: Both mRNA and protein levels of MMP-2 were inversely correlated with FEV1 percentage and FEV1/FVC percentage respectively (r=-0.577,-0.768,-0.531,-0.591,P 0.05); Similarly, a inversely correlation was also found between ICAM-1 expression (mRNA and protein) and FEV1 percentage and FEV1/FVC percentage (r=-0.584, -0.529,-0.580,-0.721,P 0.01). The expression of MMP-2 and ICAM-1 was positively correlated both on mRNA (r=0.535, P 0.01) and protein levels (r=0.664, P 0.01). CONCLUSION: Over-expressions of MMP-2 in lung tissues of patients with CDPD may lead to the abnormal support action of airway structural cells, and improve the chemotaxis and migration of inflammatory cells through ICAM-1, release certain inflammatory factors, move into the basement membrane and airway epithelial cells. In addition, highly expressed MMP-2 may participate in the inflammatory reaction of airway wall and result in the reconstitution of the lung tissues, which causes and aggravates the airway obstruction in patients with CDPD.
【CateGory Index】: R563.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 WANG Maojuan,YANG Xiaoqiong, DENG Shukai,WANG Rongli Department of Respiratory Diseases,the Affiliated Hospital, Luzhou Medical College,Luzhou 646000;Detection of matrix metalloproteinase-2, matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in serum and bronchoalveolar lavage fluid of community acquired pneumonia patients[J];Journal of Zhengzhou University(Medical Sciences);2010-06
Chinese Journal Full-text Database 10 Hits
1 SHEN Hong-tao,GU Xue-feng,WU Ping(Department of Respiratory Disease,Bengbu First people's Hospital,Anhui 233000,China);Study of the correlation between virus infection and the severity of the chronic obstructive pulmonary disease[J];Anhui Medical and Pharmaceutical Journal;2005-12
2 DENG Jing 1,CHEN Yu-qing 1,LU Yang 2,WANG Ai-hua 2 (1.Department of Respiratory Diseases,2.Laboratory of Lung Function,Affiliated Hospital of Bengbu Medical College,Anhui 233004,China);Application and clinical value of impulse oscillometry in chronic obstructive pulmonary disease[J];Journal of Bengbu Medical College;2005-01
3 CHENG Zhao zhong, XU Sheng wei, HAN Wei zhong (Department of Respiratory Diseases, The Affiliated Hospital of Qingdao University Medical College, Qingdao 266003, China);PROTECTIVE EFFECT OF AMBROXOL ON CHRONIC OBSTRUCTIVE PULMONARY DISEASE MODEL IN RATS[J];Acta Acadimae Qingdao Umvesitatis;2004-02
4 LI Tian-zhi,CHEN Zheng-tang,PENG jiang (Cancer Center of PLA,Xinqiao Hospital,the Third Military Medcial University,Chongqing,400037,China);Effect of the therapy Shenyi capsules and Guilongkechuanning capsules on the cellular immunologic function and cytotines in patients with chronic obstructive pulmonary disease[J];Chongqing Medical Journal;2005-01
5 Wang Jianping, Fan Lihua, Ji Jiansong,et al. Department of General Surgery, Lishui central hospital(the 5th affiliated hospital of Wenzhou medical college), Lishui City, Zhejiang Province 323000, China;The Perioperative Management of the Elderly Patients with Chronic Obstructive Pulmonary Disease (COPD) and Rectal Carcinoma[J];Journal of Coloproctological Surgery;2005-03
6 REN Lian-ping,SHI Ping,WANG Jia,et al.Department of Respiratory Disease, Xinhua Hospital, Shanghai Second Medical University,Shanghai 200092,China;Difference in Pulmonary Function between COPD and Overlap Syndrome Patients[J];Journal of Chinese Physician;2003-10
7 CHEN Yan, CHEN Ping, LIU Zhi-jun,et al. Department of Respiratory Medicine, The Second Xiangya Hospital, Central South University, Changsha 410011,China;The Analysis of Lung Function of 558 Patients with Chronic Obstructive Pulmonary Disease[J];Journal of Chinese Physician;2004-02
8 CAO Guo-qiang~(1), QIAN Gui-sheng~(2), XIE Zhi-jian~(1) (~(1)Department of Respiratory Diseases, Institute of Surgery Research, Daping Hospital, ~(2)Institute of Respiratory Diseases, Xinqiao Hospital, Third Military Medical University, Chongqing 400042, China);Detection of alveolar macrophage apoptosis in patients with chronic obstructive pulmonary disease[J];Acta Academiae Medicinae Militaris Tertiae;2005-17
9 LIAO Xiu-qing~1,SONG Yun-xi~1,WANG Yan~1,LI Qi~1,LIAO Wei~1,WANG Chang-zheng~1,QIAN Gui-sheng~1,ZHANG Xiao-bin~2,ZHANG Yi~2 (~1Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,~2Department of Respiratory Diseases,Fuling Central Hospital,Fuling 408000,China);Effect of cilomilast on IL-8 and TNF-α release by peripheral blood mononuclear cells in COPD patients[J];Acta Academiae Medicinae Militaris Tertiae;2006-03
10 LUO Qun1,LI Ying2,CHEN Rong-chang1,LI Yi-min1 1Guangzhou Institute of Respiratory Diseases,First Affiliated Hospital of Guangzhou Medical College,Guangzhou 510120,China;2Department of Emergency,Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,China;Changes of work of breathing in patients with acute exacerbation of chronic obstructive pulmonary disease during non-invasive positive pressure ventilation[J];Journal of Southern Medical University;2007-08
Chinese Journal Full-text Database 6 Hits
1 WANG Guang-Hui1, JIN Fa-Guang1, CHU Dong-Ling1, DUAN Li2 1Department of Respiratory Diseases, Tangdu Hospital, Xi'an 710038, China, 2Institute of Neurosciences, School of Basic Medicine, Fourth Military Medical University, Xi'an 710033, China;Expressions of matrix metalloproteinase-9 and tissue inhibitor of metalloproteinase-1 in airway remodeling of asthmatic guinea pigs[J];Journal of the Fourth Military Medical University;2006-14
2 LI Shengli 1),DANG Yuhua 2), QIU Chunguang 2) 1)Department of Cardiology, the First People's Hospital of Shangqiu City, Shangqiu 476000 2)Department of Cardiology,the First Affiliated Hospital,Zhengzhou University,Zhengzhou 450052;Changes of serum matrix metalloproteinase-9 in patients with acute coronary syndrome[J];Journal of Zhengzhou University(Science Medical);2005-05
3 DAI Li,WU Taihua.The Second People's Hospital of Panjin City,Panjin 124000,China;Effect of All-trans Retinoic Acid on Expression of Matrix Metalloproteinases in Lung Tissue of COPD Rat[J];Journal of Internal Intensive Medicine;2007-05
4 GUO Ai-ling1,TIAN Pei-chao2,WANG Huai-li2,ZHUO Zhi-hong2,QIAO Xiao-hui2,CHEN Xiao-xin2(1.Department of Surgery;2.Department of Pediatrics,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China);Expression of Matrix Metalloproteinase 9 and Tissue Inhibitor of Metalloprteinase 1 in Rats with Infectious Brain Edema[J];Journal of Applied Clinical Pediatrics;2008-11
5 XIN Xiao-feng ZHAO Ming LI Zi-ling SONG Yong SHI Yi Department of Pulmonary Diseases,Nanjing General Hospital of Nanjing Command,PLA,Nanjing 210002,China;Metalloproteinase-9/tissue inhibitor of metalloproteinase-1 in induced sputum in patients with asthma and chronic obstructive pulmonary disease and their relationship to airway inflammation and airflow limitation[J];Chinese Journal of Tuberculosis and Respiratory Diseases;2007-03
6 DU Yue, CAI Xu xu, WU Yu bin, et al. Department of Pediatrics, The Second Affiliated Hospital of China Medical University, Shenyang 110004,China;Research on the changes of expression of MMP 2 and TIMP 2 in the neonatal rats lung tissue caused by LPS[J];Chinese Journal of Perinatal Medicine;2004-06
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved