Composite of revascularized micro-morselized allograft bone and calcium phosphate cement for repairing bone defect
Gu Shou-bin1 Yan Jing-long2 Guo Tie-fang1 Hao Chen-guang1 Yang Xian-sheng2 Fu Hai-liang1 1Department of Orthopaedics Second Hospital Affiliated to Harbin Medical University Harbin 150086 Heilongjiang Province China2Department of Orthopaedics First Hospital Affiliated to Harbin Medical University Harbin 150086 Heilongjiang Province China
AIM To prepare the imitated tissue engineering artificial bone and observe and analyze the effect of composite of revascularized micro-morselized allograft bone and calcium phosphate cement in repairing segmental bone defect of rabbit radius. METHODS This experiment was conducted at the Laboratory of Tissue Engineering Harbin Medical University from March 2004 to January 2005. ① Altogether 44 3 month-old healthy Japanese flap-eared White rabbits were chosen as the receptors and randomly divided into 3 groups Tissue engineering artificial bone group Group A n=36left radius defect mixture of micro-morselized bone and calcium phosphate cement group Group B n=36 right radius defect blank group Group C n=8. ② Lognitudinal incision was created on the bilateral radius middle segment of the rabbits of Group A and Group B to expose radius. Then bilateral radius periosteum defect models with 15 mm in length were developed. ③ Micro-morselized allograft bone and calcium phosphate cement recombinant bone morphogenetic protein composite were transplanted into the rabbits of Group A . Before transplantation the composite was co-cultured for 5 days with endothelial cells and osteoblasts of blood capillary Composite of micro-morselized bone and calcium phosphate cement without cytokines or seed cells which promoting ossification and vascularization were transplanted into the rabbits of Group B There was nothing transplanted onto the bone defect region in Group C. ④ 40 ×104 U penicillin was intramuscularly injected into rabbits after operation in each group. Sample-collection was conducted in Group A and Group B at postoperative 4 8 and 12 weeks 12 rabbits once. Imaging histological and electron microscope examination were performed to observe ossification vascularization and bone defect repair of artificial bone. RESULTS Totally 44 flap-eared white rabbits were enrolled as receptors and all of them entered the stage of result analysis. ① Radiological and histological examination showed that all the bone defect in Group A and Group B were repaired to some extent . But ossified velocity ossified mass vascularized degrees and other aspects in the Group A were superior to those in Group B. ②Quantitative analysis on newly formed bone and new vessels demonstrated that percent of new vessels area and percent of newly formed bone in Group A were superior to those in Group B at postoperative 4 8 and 12 weeks P 0.05. CONCLUSION Tissue-engineered artificial bone prepared with micro-morselized allograft bone and calcium phosphate cement can speed up the progression of ossification and vascularization of artificial bone and repair bone defect effectively.
【CateGory Index】： R318
【CateGory Index】： R318