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《Chinese Journal of Clinical Rehabilitation》 2006-29
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Composite of revascularized micro-morselized allograft bone and calcium phosphate cement for repairing bone defect

Gu Shou-bin1 Yan Jing-long2 Guo Tie-fang1 Hao Chen-guang1 Yang Xian-sheng2 Fu Hai-liang1 1Department of Orthopaedics Second Hospital Affiliated to Harbin Medical University Harbin 150086 Heilongjiang Province China2Department of Orthopaedics First Hospital Affiliated to Harbin Medical University Harbin 150086 Heilongjiang Province China  
AIM To prepare the imitated tissue engineering artificial bone and observe and analyze the effect of composite of revascularized micro-morselized allograft bone and calcium phosphate cement in repairing segmental bone defect of rabbit radius. METHODS This experiment was conducted at the Laboratory of Tissue Engineering Harbin Medical University from March 2004 to January 2005. ① Altogether 44 3 month-old healthy Japanese flap-eared White rabbits were chosen as the receptors and randomly divided into 3 groups Tissue engineering artificial bone group Group A n=36left radius defect mixture of micro-morselized bone and calcium phosphate cement group Group B n=36 right radius defect blank group Group C n=8. ② Lognitudinal incision was created on the bilateral radius middle segment of the rabbits of Group A and Group B to expose radius. Then bilateral radius periosteum defect models with 15 mm in length were developed. ③ Micro-morselized allograft bone and calcium phosphate cement recombinant bone morphogenetic protein composite were transplanted into the rabbits of Group A . Before transplantation the composite was co-cultured for 5 days with endothelial cells and osteoblasts of blood capillary Composite of micro-morselized bone and calcium phosphate cement without cytokines or seed cells which promoting ossification and vascularization were transplanted into the rabbits of Group B There was nothing transplanted onto the bone defect region in Group C. ④ 40 ×104 U penicillin was intramuscularly injected into rabbits after operation in each group. Sample-collection was conducted in Group A and Group B at postoperative 4 8 and 12 weeks 12 rabbits once. Imaging histological and electron microscope examination were performed to observe ossification vascularization and bone defect repair of artificial bone. RESULTS Totally 44 flap-eared white rabbits were enrolled as receptors and all of them entered the stage of result analysis. ① Radiological and histological examination showed that all the bone defect in Group A and Group B were repaired to some extent . But ossified velocity ossified mass vascularized degrees and other aspects in the Group A were superior to those in Group B. ②Quantitative analysis on newly formed bone and new vessels demonstrated that percent of new vessels area and percent of newly formed bone in Group A were superior to those in Group B at postoperative 4 8 and 12 weeks P 0.05. CONCLUSION Tissue-engineered artificial bone prepared with micro-morselized allograft bone and calcium phosphate cement can speed up the progression of ossification and vascularization of artificial bone and repair bone defect effectively.
【Fund】: 国家自然科学基金资助(30371441)~~
【CateGory Index】: R318
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