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《Chinese Journal of Clinical Rehabilitation》 2006-31
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Regulative effects of quercetin on transforming growth factor beta 1 and connective tissue growth factor in kidney tissue in rats with unilateral ureteral occlusion

Zhang Xiao-xuan1, Zhang Xiao-dong1, Yuan Hong-ping1, Na Yu1, Miao Li-ning2, Hu Xiu-li1, Zheng Wei1, Li Gen1 1Department of Nephrology, Fourth Hospital of Jilin University, Changchun 130011, Jilin Province, China; 2Department of Nephrology, Second Hospital of Jilin University, Changchun 130041, Jilin Province, China  
AIM: To study the effects of quercetin on renal interstitial fibrosis and expressions of transforming growth factor (TGF) β1 and connective tissue growth factor (CTGF) in nephridial tissues of rats. METHODS: The experiment was performed in the Department of Third Hospital of Jilin Unversity from March 2004 to February 2005. Thirty-six Wistar rats of both sexes weighting 220-300 g were selected.(1) All rats were randomly divided into 3 groups with 12 rats in each group. Unilateral ureteral occlusion (UUO) group: UUO rat models of renal interstitial fibrosis were established according to the references. Quercetin group i.e. UUO model + quercetin treatment group. Rats were administrated with quercetin at one day before operation at the dose of 100 mg/kg once a day for 10 continuous days. Rats in the sham-operation group were separated of the left ureter after abdomen-opening without deligating the ureter.(2)Rats were executed under anesthetization at 10 days after operation, and the nephridial tissues of operating side were detected. The pathological changes of renal interstitium were studied under the light microscope. Immunohistochemical method and Western blot analysis were adopted to detect the expressions of TGF-β1 and CTGF in obstructed kidney of rats. RESULTS: A total of 36 rats were involved in the analysis of results.(1)Pathological changes of kidney in rats of each group: The renal interstitial fibrosis induced by UUO was obviously ameliorated after treatment with quercetin.(2)Immunohistochemical results of rats in each group: compared with the sham-operation group, the stained positive expressions of TGF-β1 and CTGF in the UUO group and quercetin group were significantly increased (P 0.01), and those in the quercetin group were obviously decreased than those in the UUO group [(7.41±0.93)% vs.(13.39±0.87)%,P 0.01;(7.26±0.54)% vs.(10.18±0.49)%,P 0.05](3)Results of Western blot analysis in each group: compared with the sham-operation group, levels of TGF-β1 and CTGF in obstructed kidneys of rats on the 10th day of UUO were greatly increased (P 0.01), and the relative expression amount of TGF-β1 and CTGF in the quercetin group were obviously lower than the UUO group [(2.42±0.56)% vs.(5.50±0.87)%,(3.26±1.54)% vs.(6.18±1.49)%,P 0.05]. CONCLUSION: Quercetin has anti-effect on renal interstitial fibrosis, which may be related with its down-regulation of TGF-β1 and CTGF in kidneys of rats with UUO.
【Fund】: 长春市科技局科技计划资助(04-07SF036)~~
【CateGory Index】: R285.5
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