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《Chinese Journal of Clinical Rehabilitation》 2006-33
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Effects of low frequency pulse electric-magnetic field on content of nitrogen oxide and bone remodeling in ovariectomized rats

Yang Guo-min Department of Basic Theory, College of Physical Education, Shaoxing Institute of Arts and Science, Shaoxing 312000, Zhejiang Province, China  
AIM: To establish ovariectomized rat models with osteoporosis and imitate postmenopausal osteoporosis in females, explore the effect of low frequency pulse electric-magnetic field on content of nitrogen oxide and activity of nitricoxide synthase (NOS), and the relationship with bony remodeling. METHODS: The experiment was performed at the Department of Anatomy, Shanghai University of Sport between August and November 2003. Totally 24 3-month healthy pure breed female SD rats were selected and assigned randomly into sham operation control group, gonadectomy control group and low frequency pulse electric-magnetic field group with 8 rats in each group. ①Bilateral ovaries were extracted from rats in the gonadectomy control group and the low frequency pulse electric-magnetic field group. The same volume of fat was isolated from the rats in the sham operation control group after finding out the ovaries. ②The rats in the low frequency pulse electric-magnetic field group intervened with low frequency pulse electric-magnetic field. The frequency of electric-magnetic field was 50 Hz. Tails of two rats were near to each other laid in the center of solenoid. Their heads faced to the edge of the solenoid, treating for 1 hour daily, 5 days in a week, totally for 12 weeks. ③Two fluorescent labelings were performed at different time point before being killed in rats of each group. Bone formation was observed dynamically in the two injections. ④Materials were drawn at the end of the 12th week to determine the changes of the content of nitrogen oxide and activity of NOS in serum and bone homogenate, and also detect the index of bone morphometry of lumbar vertebra. RESULTS: Totally 24 experimental rats were involved in the result analysis. ①Assaying findings of body mass, womb weight and content of serum estrogen of rats in each group after magnetic field intervention: Compared with the sham operation control group, body mass of rats in the gonadectomy control group and low frequency pulse electric-magnetic field group significantly increased (P 0.05). Womb weight and estrogen content in serum obviously decreased (P 0.05). There was insignificant difference between the two groups (P 0.05). ②Assaying findings of index of bone morphometry of each group after magnetic field intervention: Compared with the sham operation control group, relative volume ratio of bone trabecula, mean thickness of bony wall of bone trabecula and mean thickness of bony wall of cortical substance of rats in the gonadectomy control group and low frequency pulse electric-magnetic field group obviously decreased (P 0.05). Formation surface of bone trabecula, absorbing surface of bone trabecula, deposite rate of bone mineralization, percentage of cyclomycin labeled fluorescent perimeter, amount of osteoclast markedly increased (P 0.05). ③Assaying findings of content of nitrogen oxide and activity of NOS in supernatant of serum and bone homogenate after magnetic field intervention: The content of nitrogen oxide and activity of NOS in low frequency pulse electric-magnetic field group remarkably increased as compared with the gonadectomy control group (P 0.05=. CONCLUSION: Low frequency pulse electric-magnetic field can increase the content of nitrogen oxide and activity of NOS of ovariectomized rats so as to inhibit bone resorption of osteoclast and reduce high conversion rate of bone remodeling.
【CateGory Index】: R580
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