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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2007-01
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Oxidative damage and permeability change in vascular endothelial cells induced by microwave exposure

Zhong M,Xie Y,Zhang GB,Yu ZP Department of Occupational Hygiene,Third Military Medical University of Chinese PLA,Chongqing 400038,China  
AIM:To study the role of oxidative damage involved in the process of vascular permeability elevation in tissues caused by microwave irradiation.METHODS:The experiment was conducted in the Electromagnetic Radiation Biological Effect Laboratory of the Third Military Medical University of Chinese PLA from March to July in 2003.①Animal experiments:18 healthy male KM mouse were divided into three groups,namely sham group and radiation groupⅠ(parameters were measured 2 hours post-irradiation) and groupⅡ(parameters were measured 24 hours post-irradiation).All animals accepted 30 mW/cm2 microwave irradiation on whole body for 20 minutes,and 2 hours or 24 hours post-irradiation later,vascular permeability of brain,lung,liver and kidney were determined by FINa tracer test,while malondialdehyde(MDA) and superoxide dismutase(SOD) in those tissues were measured by chemical colorimetry contemporarily.②Cell experiments:The cultured confluent endothelial cells ECV304 line were exposed in 30 mW/cm2 microwave field for 20 minutes and 2,4,8,24 hours post-irradiation,percent of filtration of biotin-bovine serum albumin(BSA) on the monolayer ECV304 cells were measured in Transwell cell culture chambers,meanwhile MDA and SOD were measured by colorimetry.And ECV304 cells without irradiation were taken as contols.RESULTS:Animal experiment:①At 2 hours post-irradiation,the FINa contents of brain,lung,liver and kidney were increased 20.6%,23.8%,24.8% and 20.2% respectively compared with those of sham group(P 0.05).And 24 hours after microwave irradiation,the content were increased 31.2%,36.9%,38.2%,33.0% in radiation group(P 0.05).②In comparison with sham group,the content of SOD in tissues of brain,lung,liver and kidney decreased 13.3%,12.3%,13.64%,2.4% whereas MDA increased 7.4%,20.9%,26.0%,12.3% respectively in radiation groupⅠ(P 0.05).The contents of SOD were declined 19.5%,23.1%,20.5%,13.6% whereas those of MDA were elevated 31.6%,41.1%,49.0%,16.6% in radiation groupⅡ.Cell experiment:①Compared with controls,the vascular permeability of ECV304 cells on biotin-BSA was enhanced 63.8%,96.6% and 122% separately after microwave irradiation for 4,8,24 hours(P 0.05).②At 2,4,8,24 hours after irradiation,MDA improved 12.5%,79.6%,87.5%,110 and SOD reduced 21.8%,29.1%,36.4%,52.1% compared with control respectively(P 0.05).CONCLUSION:The disfunction of vascular endothelial cells induced by microwave exposure is contacted with oxidation-reduction dysequilibrium and oxidative damage in endothelia cells caused by microwave irradiation.
【Fund】: 国家自然科学基金资助项目(30270348)~~
【CateGory Index】: R363
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