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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2007-29
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Protection of myocardium ultrastructure by Shenfu injection in diabetes mellitus rats

Zeng J,Min S,Cao J,Wei K.1Department of Anesthesiology,Fifth Affiliated Hospital of Guangxi Medical University,Liuzhou 545001,Guangxi Zhuang Autonomous Region,China;2Department of Anesthesiology,First Affiliated Hospital of Chongqing Medical University,Chongqing 400016,China  
AIM:To investigate the effect of Shenfu injection(SFI) on the ultrastructure of myocardium in diabetes mellitus(DM) rats,and explore the possible mechanism.METHODS:The experiments were carried out in the Department of Physiology at Chongqing Medical University from December 2003 to January 2005.①Thirty Wisatr rats were randomly divided into normal control group(n =10) and DM group(n =20).②All the rats were fasted for 12 hours,then those of DM group were treated with 60 mg/kg streptozotocin(dissolved in 0.1 mol/L citric acid buffer,pH=4.2) by intraperitoneal injection,while those of normal control group were injected with equal volume of citric acid buffer solution.And 72 hours later,venous blood was sampled from rat tails to detect fasting blood glucose(FBG).Those rats appearing polydipsia,polyphagia and polyuria,whose FBG were more than 16.7 mmol/L indicated successful animal model of type 1 DM.According to stratified random,DM group was assigned to the diabetic control group(C-DM group) and the SFI treated group(S-DM group),10 rats in each.The rats in S-DM group were injected with SFI(10 mL/kg) into peritoneal cavity once a day.The rats in control group and C-DM group were injected with 10 mL/kg saline by peritoneal cavity.All the rats were fed routinely for 20 weeks.③At the end of week 20,the FBG,blood fat,serum insulin,superoxide dismutase(SOD) and malondialdehyde(MDA) in serum and myocardium were assayed.The expressions of glucose transport protein 4 mRNA on the myocardial cytomembrane were detected by immunohistochemical assay;the pathologic changes of islets of pancreas and myocardial ultrastructure of rats in three groups were also observed.RESULTS:Thirty rats were all involved in the result analysis.①Levels of FBG and serum insulin:The FBG of rats in S-DM group was remarkably lower than that of C-DM group but much higher than that of control group(P 0.01) at the end of 20 weeks.FBG of rats at the end of 3 weeks was obviously higher than that at the end of 20 weeks in S-DM group(P 0.05) but obviously lower in C-DM group(P 0.01).The serum insulin of rats in S-DM group was obviously higher than that of C-DM group but significantly lower than that of control group(P 0.05).②Blood fat level:The levels of total triglyceride,cholesterol,and low-density lipoprotein cholesterol were obviously higher in S-DM group and C-DM group than those of control group(P 0.05),and all obviously lower in S-DM group than those of C-DM group(P 0.05);The levels of high-density lipoprotein cholesterol in C-DM group and S-DM group were obviously lower than that of control group(P 0.05),and the level was significantly higher in S-DM group than in C-DM group(P 0.05).③Contents of SOD and MDA in serum and myocardium:SOD in S-DM group was much more than that of C-DM group(P 0.05),but much less than that of control group(P 0.01) while MDA was much less than that of C-DM group(P 0.05),but much more than that of control group(P 0.05).④Expressions of glucose transport protein 4 mRNA on cardiac cytomembrane of rats in S-DM group were significantly less than those of control group(P 0.01) while obviously more than those of C-DM group(P 0.01).⑤Under optic microscope,there was much less cellular edema or vacuole of pancreatic islets in rats from S-MD group compared with that of C-DM group.Under transmission electron microscope,the edema on myocardial mitochondria and endothelial cells of cardiac capillary in C-DM group was much more serious than that of S-DM group.CONCLUSION:SFI can protect the ultrastructure of myocardium in DM rats and remove oxygen-derived free radicals and resist lipid oxidation,moreover,it has the protective effect on healthy islets of pancreas in diabetic rats,which does facilitate the protection on endothelial cells of cardiac capillary and the mitochondria of myocardium,because of the increase in serum insulin and decrease in blood glucose and fat.
【CateGory Index】: R285
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1 MIN Su LIN Jing-yan ZHANG Zu-lie Department of Anesthesiology,First Affiliated Hospital,Chongqing University of Medical Sciences,Chongqing 400016,China;Protective effect of Shenfu injectio pretreatment on myocardium against ischemia-reperfusion injury in diabetic rabbits[J];Chinese Journal of Anesthesiology;2006-06
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