Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Rehabilitative Tissue Engineering Research》 2007-43
Add to Favorite Get Latest Update

Influence of thrombospondin-1 on the growth and adhesion of human hepatoma carcinoma cell line HCCLM3

Xue JF, Xie HB 1Department of General Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; 2Department of General Surgery, Fifth People's Hospital of Zhengzhou, Zhengzhou 450003, Henan Province, China  
AIM: To observe the effect of thrombospondin-1 (TSP-1) on the growth and adhesion of human hepatoma carcinoma cell line HCCLM3. METHODS: The experiment was performed in the Open Laboratory at Henan Universities from May 2006 to January 2007. ①The inhibitory effect of TSP-1 on HCCLM3 growth at logarithmic growth phase (2×107 L-1) was detected by MTT test. ②HCCLM3 growth at logarithmic growth phase were seeded onto 96-well plate covered with fibronectin, and divided into blank control group, TSP-1 group (40 mg/L TSP-1), CD36-blocked group and CD47-blocked group, which were incubated in incubator with 0.05 volume fraction CO2 at 37 ℃ for 30 minutes after adding 5 mg/L corresponding monoclonal antibody, following by TSP-1. Seventy-two hours later, the effect of TSP-1 on cell adhesion was detected by MTT test and cell counting. RESULTS: ①The inhibitory effect of TSP-1 on HCCLM3 growth was enhanced with the increased concentration and time. There was no significantly difference between 40 mg/L and 50 mg/L. The maximum effect was at 72 hour. ②The cellular adhesion in experimental groups was significantly reduced compared with that in control group (P 0.05). There was no difference in cellular adhesion among TSP-1 group, CD47-blocked group and CD36-blocked group (P 0.05). CONCLUSION: The inhibition of TSP-1 to the growth of HCCLM3 cell depends on the dose and time. TSP-1 could inhibit the adhesion to extracellular matrix of HCCLM3, which may be not related with CD36 and CD47.
【CateGory Index】: R735.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 WEI Fang, SUN Wei, WANG Qiang, LIU Li.Shengjing Hospital of China Medical University, Shenyang 110003, China;Expressions and significances of thrombospondin-1 and hypoxia-inducible factor-1 alpha in gastric cancer[J];The Journal of Practical Medicine;2009-12
【Citations】
Chinese Journal Full-text Database 2 Hits
1 XUE Jian-feng~1, PANG Zhi-gang~2, LIU Chao~2, WANG Guang-tian~2, LIU Fu-bao~1, PENG Shu-you~1 (~1Department of Surgery, The Second Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, China;~2Department of Surgery, The Second Affiliated Hospital, School of Medicine, Zhengzhou University, Zhengzhou 450003, China);Significance of expression of thrombospondin-1 and receptor-CD36 in hepatocellular carcinoma[J];Chinese Journal of Pathophysiology;2006-02
2 LI Yan, TANG Zhaoyou, YE Shenglong, LIU Yinkun, CHEN Jie, XUE Qiong, HUANG Xiaowu, CHEN Jun, BAO Weihua, YANG Jiong, GAO Dongmei. Liver Cancer Institute and Zhong Shan Hospital of Fudan University, Shanghai, 200032, China;Establishment of human hepatocellular carcinoma cell line with spontaneous pulmonary metastasis through in vivo selection[J];National Medical Journal of China;2002-09
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Liu Hu,Ye Sheng -Long ,Li Wen-Chang ,Li Yan,Liu Yin-Kun,Qin Lun-Xiu,Qiu Shuang -Jian,Sun Rui-Xia,Chen Jie,Tang Zhao-YouLiver Cancer Institute /Zhongshan Hospital,Fudan University,Shanghai,200032,P.R.China;Quantitative Analysis of Expression of TEY1 Gene in Liver Cancer Cells with Different Metastatic Potential[J];Chinese Journal of Cancer;2003-06
2 PENG Zhihong~1, TANG Bo~2, YANG Jianmin~1, SI Suihai~1, CHEN Wensheng~1, FANG Dianchun~1, LUO Yuanhui~1 (~1Gastroenterology Research Center, ~2Department of General Surgery, Southwest Hospital, Third Military Medical University, Chongqing 400038, China);Effects of metastasis suppressor gene KAI1 on adhesion forces of MHCC97-H hepatocellular carcinoma cells with high metastatic potential[J];Acta Academiae Medicinae Militaris Tertiae;2005-23
3 XUE Jian-feng1,LIU Kang-dong21.Department of General Surgery,First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,P.R.China2.Department of Pathophysiology,Basic Medical College,Zhengzhou University,Zhengzhou 450052,P.R.China;Influnce of thrombospondin-1 on growthing and microvessel density in null mice tumor modle of human hepatic carcinoma cell line HCCLM3[J];Chinese Journal of Cancer Prevention and Treatment;2008-15
4 WU Xu-dong1,CHEN Wei-chang1,ZHU Shu-yang2,YE Jian-xin1 1.Department of Gastroenterology,First affiliated Hospital of Soochow University,Suzhou 215006,P.R.China2.Department of Pediatrics,North Courtyard of First People's Hospital of Chenzhou City,Chenzhou 423000,P.R.China;Effect of RNA interference targeting at peroxisome proliferator-activated receptor gamma gene on angiogenesis of hepatocellular carcinoma in nude mice[J];Chinese Journal of Cancer Prevention and Treatment;2009-07
5 YANG Xia1,WANG Qing-song1,CHENG Rui1,LI Chao-yang2,CAI Wei-bin1,YANG Zhong-han1,LI Min-you3,HE Guang-yao1,GAO Guo-quan1(1 Department of Biochemistry,Basic Medical College,2 Zhongshan Ophthalmic Center,Sun Yat-sen University,Guangzhou 510080,China; 3 Guangzhou Qiyuan Biotech Co.Ltd,Guangzhou 510630,China.);Plasminogen kringle 5 deletion mutantⅠ inhibits the neovascularization and growth of the mouse hepatocarcinoma[J];Chinese Journal of Pathophysiology;2008-02
6 JIANG Ping,JIN Zhou-xiang,WANG Ji-sheng,XU Lu-bai,ZHANG Wei,ZHU Shao-jun(Department of Surgery,The Second Affiliated Hospital of Wenzhou Medical College,Wenzhou 325027,China.);Therapeutic effect of 3TSR on gastric carcinoma in nude mice[J];Chinese Journal of Pathophysiology;2008-08
7 LIU Hu,YE Sheng-long,XUE Qiong,et al.Liver Cancer Institute & Zhong Shan Hospital,Fudan University;Key Laboratory of Carcinogenesis and Cancer Invasion,Ministry of Education,Shanghai 200032,China;The establishment of a spontaneous metastatic model of rat liver cancer in nude mice[J];Chinese Journal of Experimental Surgery;2004-01
8 WENG Yong-qiang, QIU Shuang-jian, TANG Zhao-you, YE Sheng-long, LIU Yin-kun, FAN Jia, SUN Rui-xia, ZHAO Yan. Liver Cancer Institute, Zhongshan Hospital, Fudan University, Shanghai 200032, China;Changes in the immune function of dendritic cells (DC) derived from HBV-related hepatocellular carcinoma (HCC) patients' peripheral blood monocytes (PBMC) pulsed with tumor antigen[J];Chinese Journal of Hepatology;2005-05
9 CHENG Wen-wu, SONG Ming-zhi, XUE Qiong, SHEN Ye-hua. Cancer Hospital, Fudan University, Shanghai 200032, China;Effects of strengthening and regulating spleen-qi recipe on nude mice with metastatic human hepatocellular carcinoma[J];Chinese Journal of Hepatology;2005-06
10 Xue JF, Xie HB 1Department of General Surgery, First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, China; 2Department of General Surgery, Fifth People's Hospital of Zhengzhou, Zhengzhou 450003, Henan Province, China;Influence of thrombospondin-1 on the growth and adhesion of human hepatoma carcinoma cell line HCCLM3[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2007-43
【Co-references】
Chinese Journal Full-text Database 3 Hits
1 MAO Wenbin, SHAO Zengwu. Department of Orthopaedics of Union Hospital of Tongji Medical College, Huazhong Unversity of Science And Technology. Wuhan 430022 China;Relationship of the expression of iNOS and HIF-1α with angiogenesis in osteosarcoma[J];Chinese Journal of Bone Tumor and Bone Disease;2006-04
2 LI Dong-yan,HAO Min(Dept of Obstetrics and Gynecology,Second Clinical College,Shanxi Medical University,Taiyuan 030001,China);Vascular endothelial growth factor and thrombosponding involved in ectopic endometrium[J];Journal of Shanxi Medical University;2007-01
3 GUO Ke-qin, YU Ru-tong, GAO Wen-chang (Department of Neurosurgery, Affiliated Hospital of Xuzhou Medical College,Xuzhou,Jiangsu 221002, China);Study of expressions for hypoxia-inducible factor-1 alpha and vascular endothelial growth factor in human glioma and their impact on prognosis[J];Acta Academiae Medicinae Xuzhou;2003-03
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 LI Yan TANG Zhaoyou YE Shenglong,Liver Cancer Institute,Zhongshan Hospital,Fudan University,Shanghai 20032,P.R.China;Isolation and establishment of human hepatocellular carcinoma cell clones with various metastatic potentiais[J];Chinese Journal of Hepatobiliary Surgery;2001-11
2 ZHANG Siwei, LI Liandi, LU Fengzhu, et al. National Office of Cancer Prevention and Treatment, Beijing 100021;Mortality of primary liver cancer in China from 1990 through 1992[J];CHINESE JOURNAL OF ONCOLOGY;1999-04
3 Tang Zhao-you et al;HUMAN HEPATOCELLULAR CARCINOMA (HCC) MODEL IN NUDE MICE-ESTABLISHMENT OF HCC TISSUE MODEL AND ITS APPLICATION IN EXPERIMENT RESEARCH[J];Tumor;1986-01
4 LIANG Guang-bo 1, ZHANG Guo-ping 1, JIN Hui-ming 1, TANG Da-kan 2 ( 1Department of Physiology and Pathophysiology,? 2?Department of Medical Bioengineering,Shanghai Medical College,Fudan University,Shanghai 200032,China);Establishment of quantitative measurement method of cell migration in vitro and it's application[J];Chinese Journal of Pathophysiology;2004-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved