Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Rehabilitative Tissue Engineering Research》 2008-03
Add to Favorite Get Latest Update

Cycle of mesenchymal stem cells from femoral metaphysis in patients with alcohol-induced osteonecrosis of the femoral head

Sun Wei,Wang Bai-liang,Li Zi-rong,Shi Zhen-cai,Shi Shao-hui,Cheng Li-ming,Wang Ran-dong Bone Circulation and Osteonecrosis Insti-tute,Department of Orthopaedics,China-Japan Friend-ship Hospital,Beijing 100029,China  
AIM:Mesenchymal stem cells(MSCs) can play important roles in the pathogenesy of alcohol-induced osteonecrosis of femoral head.This study was aimed to detect MSCs cycle and proliferation activity in the region of proximal femoral head and neck and femoral metaphysis of the patients with alcohol-induced osteonecrosis with flow cytometry(FCM).METHODS:From February to May 2005,the research was performed in Bone Circulation and Osteonecrosis Institute of China-Japan Friendship Hospital.Totally 10 patients diagnosing as having alcohol-induced osteonecrosis of femoral head were enrolled in the experimental group(ARCO Ⅱ,Ⅲ A),including 8 males and 2 females,averagely 36.9 years(28-46 years).Eleven femoral neck fracture(less then 1 week) patients without the history of osteonecrosis of femoral head and alcohol drinking were enrolled in the control group,including 8 males and 3 females,averagely 60 years(55-65 years).The bone marrow blood(3-5 mL) was collected with the agreement of patients.MSCs were harvested and centrifugated by density gradient centrifugation,and then selected by the adhesive method.Proliferation and cycle of second-generation MSCs were detected with FCM.RESULTS:Totally 21 testees were involved in the result analysis.The results indicated that percentage of cells at the G0/G1 phase was higher,but lower at the(S+G2/M) phase and the proliferation ability was reduced in the experimental group as compared with the control group(P 0.05).CONCLUSION:①The proliferation ability of MSCs may decrease in the alcohol-induced osteonecrosis of femoral head.②Alcohol can result in osteonecrosis by decreasing proliferation activity or differentiation of MSCs to change the differentiating direction.
【Fund】: 首都医学发展基金重大联合项目(2002-1007);; 国家自然科学基金面上项目(30672117);; 中日友好医院博士启动课题(20056150);; 北京医学会骨科分会-默沙东骨质疏松科研项目~~
【CateGory Index】: R681.8
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 Shi Shao-hui1,Li Zi-rong2,Sun Wei2,Wang Bai-liang2,Pan Lin3,Wang Ran-dong1 1Department of Orthopedics,Aviation Industry Central Hospital,Beijing 100012,China;2Center for Bone Necrosis and Joint Reconstruction,China-Japan Friendship Hospital,Beijing 100029,China;3Institution of Clinical Medicine,China-Japan Friendship Hospital,Beijing 100012,China;Mechanism of lipid metabolism in alcohol-induced and steroid-induced osteonecrosis of the femoral head[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2011-17
Chinese Journal Full-text Database 10 Hits
1 SHEN Jun-jie1,LI Hua-can1,LE Yu-min2(1.Department of Medical Imaging,2.Department of Rehabilitation,The Second Affiliated Hospital of Fujian College of Traditional Chinese Medicine,Fuzhou Fujian 350001,China);Digital subtraction angiography in interventional therapy for avascular necrosis of femoral head[J];Journal of Bengbu Medical College;2009-01
2 ZHENG Xia-ru,ZHANG Bai-qiu,SUN Peng Rehabilitation Department,The Hospital of Longgang Central,Shenzhen,518116,China Department of Interventional Radiology,Changchun Central City Hospital,Jinlin,130051 China;Physical therapy combined with interventional treatment for early stage of femoral head necrosis[J];Contemporary Medicine;2009-29
3 XUAN Jing-jing,ZHANG Yuan-chao (Department of Rheumatology and Immunology,Provincial Hospital Affiliated to Shandong University,Ji'nan 250021,China);Glucocorticoids and femoral head osteonecrosis in systemic lupus erythematosus patients[J];World Clinical Drugs;2011-01
4 WANG Songhao (Longhu People's Hospital of Shantou,Guangdong Shantou 515041,China);Clinical Analysis of the Imaging of X-ray and CT in Patients with Femoral Head Necrosis[J];Hebei Medicine;2010-08
5 ZHANG De-zhou,YI Xue-bing,ZHONG Jian,et al.(Department of Radiology,Sichuan Province Orthopedics Hospital,Chengdu 610041,China);CT Analysis of Different Causes of the Femoral Head Ischemic Necrosis[J];West China Medical Journal;2008-06
6 WEI Biao-fang(Linyi City People's Hospital,Linyi,China 276003);RELATIONSHIP BETWEEN GENE POLYMORPHISMS AND SYNDROME TYPES IN STEROID-INDUCED FEMORAL HEAD OSTEONECROSIS[J];Journal of Shandong Medical College;2011-05
7 ZHU Juan,LONG Li-ling,XIAO Zeng-ming(Department of Radiology,the First Affilated Hospital of Guangxi Medical University,Nanning 530021,China);Usefulness of Imaging Techniques in Diagnosis of Corticosteroid-induced Avascular Necrosis of Femoral Head[J];Journal of Practical Radiology;2004-11
8 Wang Jianzhong, Wang Kunzheng, Shi Zhibin, Zhang Mingyu(Department of Orthopaedics, the Second Affiliated Hospital,Medical School of Xi'an Jiaotong University, Xi'an 710004, China);Effect of long-term administration of glucocorticoid on expression level of OPG/RANKL mRNAs in bone tissues of femoral head of rats[J];Journal of Xi'an Jiaotong University(Medical Sciences);2008-06
9 Shi Yue1,Yin Wen-zhou2,Chen Qi-zhong3,Wang An-ming1,Liu Jun-quan4,Liu Hong-bin5,Gao Cong-jing1 1Department of Interventional Radiology,3Department of Emergency,4Department of Medical Laboratory,5Department of Orthopedics,the 97th Hospital of Chinese PLA,Xuzhou 221004,Jiangsu Province,China;2Department of Interventional Radiology,Xuzhou Hospital of Traditional Chinese Medicine,Xuzhou 221003,Jiangsu Province,China;Arterial perfusion combined with regional injection of autologous bone marrow mesenchymal stem cells for femoral head necrosis[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2011-06
10 LI Tingsong,XIAO Zengming,ZHAN Xinli et al.Department of Orthopeadie,First Affiliated Hospital,Guangxi Medical University,Nanning 530021,CHINA;The changes in lipids,coagulation-fibrinolysis and apoptosis in rabbits with steroid-induced necrosis of the femoral head[J];Jiangsu Medical Journal;2010-08
Chinese Journal Full-text Database 10 Hits
1 WANG Zhong, GAO Yi, WANG Yan, PAN Ming-xin. Department of General Surgery, Zhujiang Hospital, Southern Medical University, Guangzhou 510282, China;Isolation, cultivation and biological identification of human bone marrow mesenchymal stem cells[J];Chinese Journal of Neuromedicine;2006-10
2 ZHAO Ying(College of Life Sciences,Chongqing Normal University,Chongqing 400047,China);Isolation,Cultivation and Identification of SD Rat Bone Marrow-Derived Mesenchymal Stem Cells[J];Journal of Chongqing Normal University(Natural Science Edition);2008-01
3 Peng Wuxun1,Wang Lei2,Deng Jin1,Li Yanlin3,Gong Yuekun3,Li Shihe3(1Department of Emergency and Trauma Surgery,Affiliated Hospital,Guiyang Medical College,Guiyang,Guizhou Province,550004;2Institute of Occupational Medicine,Guizhou Center for Disease Control and Prevention,Guiyang,Guizhou Province,550004;3Department of Orthopaedics,First Affiliated Hospital,Kunming Medical College,Kunming,Yunnan Province,650032,China);A tissue engineering bone to repair femoral head necrosis[J];Acta Academiae Medicinae Militaris Tertiae;2010-07
4 ZHANG Yi-Fu1, LIU Jian1, MENG Guo-Lin1, YUAN Zhi1, L Rong1, YUAN Peng2 1PLA Institute of Orthopaedics, Xijing Hospital, Fourth Military Medical University, Xi'an 710033, China, 2Department of Obstetrics and Gynecology, Urumqi Military General Hospital, Urumqi 810000, China;Change of hepatic lipase and its meaning during the glucocorticoid- induced avascular necrosis of femoral head in rabbits[J];Journal of the Fourth Military Medical University;2006-08
5 WANG He ming,WANG Li,LI Nan(Orthopaedic and Traumatology department of FCTCM,Fuzhou,Fujian 350003, China);Study the Influence of Morinda Officinalis How on the Differentiation from Marrow Stroma Cell to Osteoblast[J];Journal of Fujian College of Traditional Chinese Medicine;2004-03
6 QI Zhen-xi1, YU Can-ming2(1 .Depart ment of Scientific Research,FCTCM,Fuzhou ,Fujian 350003 ,China ;2 . Orthopaedic and Traumatology Depart ment ,FCTCM,Fuzhou ,Fujian 350003 ,China);Characteristics of Hemorheology and Oxygen Free Radicals Metabolismin Model Rats of Alcohol-Induced Avascular Necrosis of The Femoral Head[J];Journal of Fujian College of Traditional Chinese Medicine;2005-04
7 Guo kunliang, An Hong, Jiang Dianming, et al. Department of Orthopaedics, The first Affiliated Hospital of Chongqing Medical Universidy,Chongqing 400016;Establishment on animal model of alcohol-induced avascular necrosis of the femoral head[J];Guizhon Medical Journal;2002-10
8 ZHANG Jie,ZOU Xiaoping.Department of Gastroenterology,Gulou Hospital Affiliated to Medical College of Nanjing University,Najing 210008,Jiangsu,P.R.CHINA;Effect of alcohol on hypoxia and anti-apoptosis protein in gastric cancer cells[J];Hainan Medical Journal;2009-04
9 WU Xuejian 1) , YIN Wanle 1) ,LI Yuebai 2) ,WANG Yisheng 1) 1)Department of Orthopaedics, the First Affiliated Hospital, Zhengzhou University, Zhengzhou 450052 2)Department of Biochemistry, College of Basic Medical Sciences, Zhengzhou University, Zhengzhou 450052;Effects of alcohol on mRNA expression of PPARγ and osteocalcin in human bone marrow mesenhymal stem cells[J];Journal of Zhengzhou University(Medical Sciences);2006-06
10 WANG Shaohua1),WANG Yisheng1),LI Yuebai2),ZHAO Guoqiang3)1)Departmemt of Orthopaedic Surgery,the First Affiliated Hospital,Zhengzhou University;Open Laboratory of Unode Science of Clinical Medicine of Henan Province,Zhengzhou 450052 2)Department of Biochemistry,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001 3)Department of Microbiology and Immunology,College of Basic Medical Sciences,Zhengzhou University,Zhengzhou 450001;Effects of RNAi for target on PPARγ to suppress adipogenic differentiation of MSCs of rabbit induced by ethanol[J];Journal of Zhengzhou University(Medical Sciences);2009-02
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 LI Zi-rong~*, SUN Wei, QU Hui, ZHOU Yi-xiong, DOU Bao-xin, SHI Zhen-cai, ZHANG Nian-fei, CHENG Xiao-guang, WANG Da-li, GUO Wan-shou.~* Center for Osteonecrosis & Joint-preserving Reconstruction, China-Japan Friendship Hospital, Beijing 100029, China;Clinical research of correlation between osteonecrosis and steroid[J];Chinese Journal of Surgery;2005-16
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved