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Cycle of mesenchymal stem cells from femoral metaphysis in patients with alcohol-induced osteonecrosis of the femoral head

Sun Wei,Wang Bai-liang,Li Zi-rong,Shi Zhen-cai,Shi Shao-hui,Cheng Li-ming,Wang Ran-dong Bone Circulation and Osteonecrosis Insti-tute,Department of Orthopaedics,China-Japan Friend-ship Hospital,Beijing 100029,China  
AIM:Mesenchymal stem cells(MSCs) can play important roles in the pathogenesy of alcohol-induced osteonecrosis of femoral head.This study was aimed to detect MSCs cycle and proliferation activity in the region of proximal femoral head and neck and femoral metaphysis of the patients with alcohol-induced osteonecrosis with flow cytometry(FCM).METHODS:From February to May 2005,the research was performed in Bone Circulation and Osteonecrosis Institute of China-Japan Friendship Hospital.Totally 10 patients diagnosing as having alcohol-induced osteonecrosis of femoral head were enrolled in the experimental group(ARCO Ⅱ,Ⅲ A),including 8 males and 2 females,averagely 36.9 years(28-46 years).Eleven femoral neck fracture(less then 1 week) patients without the history of osteonecrosis of femoral head and alcohol drinking were enrolled in the control group,including 8 males and 3 females,averagely 60 years(55-65 years).The bone marrow blood(3-5 mL) was collected with the agreement of patients.MSCs were harvested and centrifugated by density gradient centrifugation,and then selected by the adhesive method.Proliferation and cycle of second-generation MSCs were detected with FCM.RESULTS:Totally 21 testees were involved in the result analysis.The results indicated that percentage of cells at the G0/G1 phase was higher,but lower at the(S+G2/M) phase and the proliferation ability was reduced in the experimental group as compared with the control group(P 0.05).CONCLUSION:①The proliferation ability of MSCs may decrease in the alcohol-induced osteonecrosis of femoral head.②Alcohol can result in osteonecrosis by decreasing proliferation activity or differentiation of MSCs to change the differentiating direction.
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