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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2008-23
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Preparation of chitosan scaffold tube

Chen Gang, Shi Wen-jun Department of Thoracic Surgery, the Second Affiliated Hospital of Chinese Medical University, Shenyang 110003, Liaoning Province, China  
BACKGROUND: Chitosan is commonly prepared using emulsion freeze-drying methods, which is complexly operated. OBJECTIVE: To introduce a simple methods for the preparation of chitosan and analyze its biological characteristic. DESIGN, TIME AND SETTING: Controlled observation experiments were carried out in the Central Laboratory, the Second Affiliated Hospital of Chinese Medical University (Shenyang, Liaoning, China) from April to December in 2007. MATERIALS: Ten Wistar rats of 8 weeks old and SPF grade were adopted. Chitosan powder in separated packs, with 99% purity and 99% deacetylation degree were the product of Shandong Jinan Ocean Bio-Product Co., Ltd. (China). METHODS: Acetic acid solution was added into chitosan to prepare 8% chitosan acetic acid hydrosol, which was then stayed for one day. Flowing the immersion into the hydrosol and immediate dislodgement, plastic tube was soaked in 1 mol/L sodium hydroxide for 3 minutes. Hydrosol out of the tube etiolated and coagulated into jells, the tube was removed when the jells were semi-dried. Subsequent to complete drying, chitosan tubes were prepared as an internal diameter of 5 mm, and were cut into many segmentations (length 2 mm). The prepared tubes were subcutaneously implanted into rat muscular tissues at right McBurney point, while rat left muscular tissues were only incised and sutured, taking as controls. MAIN OUTCOME MEASURES: Gross observation of chitosan tube, degradation percentage of chitosan tubes in rat muscular tissue, and tissue inflammation change around chitosan tube detected by hematoxylin-eosin stain. RESULTS: The surface of chitosan tube was smooth and the tenacity was good. Intramuscular implantation test showed that 50% and 90% chitosan tubes were absorbed by tissues in 4 weeks and 8 weeks postoperatively. Inflammatory reaction surrounding tubes was decreased gradually. CONCLUSION: It gives a new kind of way to prepare chitosan tube, with few materials, low cost, simple operation and short time. The prepared chitosan tubes approve the good histocompatibility and biological degradation.
【Fund】: 卫生部立项资助课题 课题名称:肺组织瓣重建气管食管的应用研究。(WKZ-2000-1-17)~~
【CateGory Index】: R318.08
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