Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Rehabilitative Tissue Engineering Research》 2008-23
Add to Favorite Get Latest Update

Preparation of chitosan scaffold tube

Chen Gang, Shi Wen-jun Department of Thoracic Surgery, the Second Affiliated Hospital of Chinese Medical University, Shenyang 110003, Liaoning Province, China  
BACKGROUND: Chitosan is commonly prepared using emulsion freeze-drying methods, which is complexly operated. OBJECTIVE: To introduce a simple methods for the preparation of chitosan and analyze its biological characteristic. DESIGN, TIME AND SETTING: Controlled observation experiments were carried out in the Central Laboratory, the Second Affiliated Hospital of Chinese Medical University (Shenyang, Liaoning, China) from April to December in 2007. MATERIALS: Ten Wistar rats of 8 weeks old and SPF grade were adopted. Chitosan powder in separated packs, with 99% purity and 99% deacetylation degree were the product of Shandong Jinan Ocean Bio-Product Co., Ltd. (China). METHODS: Acetic acid solution was added into chitosan to prepare 8% chitosan acetic acid hydrosol, which was then stayed for one day. Flowing the immersion into the hydrosol and immediate dislodgement, plastic tube was soaked in 1 mol/L sodium hydroxide for 3 minutes. Hydrosol out of the tube etiolated and coagulated into jells, the tube was removed when the jells were semi-dried. Subsequent to complete drying, chitosan tubes were prepared as an internal diameter of 5 mm, and were cut into many segmentations (length 2 mm). The prepared tubes were subcutaneously implanted into rat muscular tissues at right McBurney point, while rat left muscular tissues were only incised and sutured, taking as controls. MAIN OUTCOME MEASURES: Gross observation of chitosan tube, degradation percentage of chitosan tubes in rat muscular tissue, and tissue inflammation change around chitosan tube detected by hematoxylin-eosin stain. RESULTS: The surface of chitosan tube was smooth and the tenacity was good. Intramuscular implantation test showed that 50% and 90% chitosan tubes were absorbed by tissues in 4 weeks and 8 weeks postoperatively. Inflammatory reaction surrounding tubes was decreased gradually. CONCLUSION: It gives a new kind of way to prepare chitosan tube, with few materials, low cost, simple operation and short time. The prepared chitosan tubes approve the good histocompatibility and biological degradation.
【Fund】: 卫生部立项资助课题 课题名称:肺组织瓣重建气管食管的应用研究。(WKZ-2000-1-17)~~
【CateGory Index】: R318.08
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 8 Hits
1 LU Fengqi,CAO jing,LIN Zhiyong,et al (Dept.of Pharmacy,Shandong Medical University);HISTOPATHOLOGICAL INVESTIGATION ON THE MEMBRANE FOR PREVENTION OF POSTSURGICAL ADHESION[J];ACTA ACADEMIAE MEDICINAE SHANDONG;2000-02
2 WANG Feng , ZHUANG ZHao xia, LU Feng qi (Dept.of Periodontics,School of Dentistry,Shandong University);Experimental study on tissue reactions and degradation of chitosan membrane in rabbits[J];Acta Academiae Medicinae Shandong;2002-04
3 GAO Ping,BAO Shi-lan,LIU Xin,SHI Hong-dao Tianjin Medical Biomaterial Inspection & Research Center, Tianjin Institute of Medical & Pharmaceutical Science,Tianjin 300070,China;The Study of the Degradation for Medical Chitosan in Different Degree of Crosslinking[J];Biomedical Engineering and Clinical Medicine;2002-04
4 KONG Li-Jun~1,AO Qiang~2,XI Jing~1,ZHANG Ling~1,GONG Yan-Dao~1,ZHAO Nan-Ming~1 and ZHANG Xiu-Fang~ 1* 1 Department of Biological Sciences and Biotechnology,State Key Lab of Biomembr ane and Membrane Biotechnology,Tsinghua University,Beijing 100084,China 2 Institute of Neurological Disorder,Tsinghua University,Beijing 100084,China;Proliferation and Differentiation of MC 3T3-E1 Cells Cultured on Nanohydroxya patite/chitosan Composite Scaffolds[J];Chinese Journal of Biotechnology;2007-02
5 Cheng Rui,Li Anmao∥Hepatobiliary Department of Xijing Hospital,Fourth Military Medical University,Xian 710032,China,;The tube produced from chitosan and discussion on its clinical use[J];Journal of Xi'an Medical University(Chinese);2000-06
6 ZHANG Zu-fei,ZHOU Jian-ping~(*),HUO Mei-rong(Department of Pharmaceutics,China Pharmaceutical University,Nanjing 210009,China);Chitosan Microspheres Drug Delivery Systems[J];Progress in Pharmaceutical Sciences;2006-06
7 XU Ying-xin, YIN Tai, LI Rui-xin, et al. Institute of General Surgery, The General Hospital of PLA, Beijing 100853, China;Elementary study on construction of engineered blood vessel using chitosan-fibrin gel as scaffold[J];Chinese Journal of Rehabilitation Theory & Practice;2005-08
8 HU Xueqing,HAN Chunmao,SHI Haifei,et al.Department of Burn,Second Affiliated Hospital of Zhejiang University,Hangzhou Zhejiang,310009,P.R.China;PRIMARY STUDY ON HISTOCOMPATIBILITY OF THREE KINDS OF COLLAGEN-CHITOSAN POROUS SCAFFOLDS[J];Chinese Journal of Reparative and Reconstructive Surgery;2005-10
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 SHEN Hong-Liang,WANG Qiang ,QIAN Fang,LIU Gao-Lin,LI Yang(Department of General Surgery,Changzheng Hospital ,Second Military Medical University,Shanghai 200003,China; Department of Pharmacy,Changzheng Hospital);Preparation and in vitro release of levofloxacin carboxymethyl chitosan sustained-releasing microsphere[J];Academic Journal of Second Military Medical University;2004-05
2 ZHAO Li-rui~(1,2) SUN Duo-xian~1 LIU Man-ying~2(1.School of Chemical Engineering and technology,Tianjin University,Tianjin 300072,China;2.School of basic chemical sciences,Hebei medical university,Shijiazhuang 050017,China);The Performances and Applications in Biomedicine of Carboxythmethylchitosan[J];Chinese Polymer Bulletin;2007-08
3 CHEN Hong-li , CHEN Han , Moldir , et al . Institute of Biomedical Engineering , Chinese Academy of Medical Sciences and Peking Union Medical College ; Tianjin Key Laboratory of Biomaterials , Tianjin 300192 , China;Preparation and characteristics of collagen-chitosan complex film with embedded PLGA microspheres[J];International Journal of Biomedical Engineering;2006-04
4 HU Xue1,2,WEI Wei 1,LEI Jian-du 1,MA Guang-hui 1,SU Zhi-guo 1,WANG Hua-jun 2(1.State Key Lab.Biochem.Eng.,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100080,China;2.School of Civil and Environment Engineering,University of Science and Technology Beijing,Beijing 100083,China);Preparation of Uniform Size Chitosan Microspheres by a T-shaped Microchannel Device[J];The Chinese Journal of Process Engineering;2008-01
5 ZHOU Xie-lai\+1, ZHANG Zhi-liang\+1, JIANG Su-jun\+2,NI You-di\+1, RU Jin-quan\+1, DU Hong-ying\+1, ZHENG Ting\+1, HU Qiao-ling\+2 (1. Medical College of Hangzhou Teachers College, Hangzhou 310012, China; 2. College of Materials Science and Chemical Engineering, Zhejiang University, Hangzhou 310027, China);Effect of chitosan/gelatin blending film on the formation of peritoneal adhesion in rats[J];Journal of Hangzhou Medical College;2003-02
6 Shen Hongliang, Wang Qiang, Qian Fang et al. Department of Minimal Wound Surgery, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China;Treatment of soft tissue wound with seawater immersion by administration of levofloxacin-chitosan microsphere at early stage[J];Medical Journal of Chinese People's Liberation Army;2006-03
7 CHEN Chu, GONG Leilei, CHENG Qiong, DING Fei (Department of Neurobiology, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Jiangsu 226001);Effects of chitooligosaccharides on the crushed common peroneal nerve of rabbits[J];Medical Journal of Communications;2009-03
8 WANG Ling-Chong,CHEN Xi-Guang~(**),LIU Cheng-Sheng,MENG Xiang-Hong,LIU Chen-Guang,HE Jun,YU Le-Jun,NI Si-Liang(College of Marine Life Sciences,Ocean University of China,Qingdao 266003,China);Behavior and Application of Chitosan Membranes in the Medical Field[J];Periodical of Ocean University of China;2006-02
9 KONG Li-Jun~1,AO Qiang~2,XI Jing~1,ZHANG Ling~1,GONG Yan-Dao~1,ZHAO Nan-Ming~1 and ZHANG Xiu-Fang~ 1* 1 Department of Biological Sciences and Biotechnology,State Key Lab of Biomembr ane and Membrane Biotechnology,Tsinghua University,Beijing 100084,China 2 Institute of Neurological Disorder,Tsinghua University,Beijing 100084,China;Proliferation and Differentiation of MC 3T3-E1 Cells Cultured on Nanohydroxya patite/chitosan Composite Scaffolds[J];Chinese Journal of Biotechnology;2007-02
10 Xiao Hai jun 1 Hou Chun lin 1 Gu Qi sheng 2 Liu Ya ping 11.Department of Orthopedics,Changzheng Hospital (Shanghai 200003);2.Qisheng Institute of Biomaterial and Technology (Shanghai 201106);Progress of study on prevention of postoperative adhesion by use of chitosan membrane[J];Shanghai Journal of Biomedical Engineering;2004-03
China Proceedings of conference Full-text Database 1 Hits
1 CHEN Hongli, CHEN Han, Moldir, LIU Lingrong, Zhang qiqing(Institute of Biomedical Engineering, Chinese Academy of Medical Sciences and Peking Union Medical College, Tianjin Key Laboratory of Biomaterials, Tianjin 300192, China);The preparation and characteristics of collagen-chitosan complex film containing PLGA microspheres[A];[C];2006
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wang Aiqin; Xue Zuoliang ;Zhao Shengmin (Lanzhou Institute of Chemical Physics,Chinese Academy of Science,Lanzhou 730000)Cao Nong; Jia Baoquan (The First Affiated Hospital,Lanzhou Medical College);Studies on Preparation and Properties of Chitosan Membrane of Matrix[J];BEIJING BIOMEDICAL ENGINEERING;1996-01
2 ZHENG Ai-ping ▲, YU Shao-yun, ZHAO Ying,WU Feng-lan △ (Department of Pharmaceutics, Peking University School of Pharmaceutical sciences, Beijing 100083, China);Preparation and properties of 5-fluorouracil-loaded chitosan microspheres for the intranasal administration[J];Journal of Peking University(Health Sciences);2004-03
3 Lin Ruixun Yiang Suhong Zhang Mushan Guangzhou Institute of Chemistry,Academia Sinica;THE METHOD FOR DETERMINATION OF DEGREE OF DEACETYLATION OF CHITOSAN[J];Guangzhou Chemistry;1992-04
4 Shao Jian,Yang Yumin (Nantong Medical College,Nantong 226001);HYGROSCOPICITY,MOISTURE RETENTION AND ANTI-MICROBIAL ACTIVITY OF OLIGO-GLUCOSAMINE[J];CHINESE JOURNAL OF MARINE DRUGS;2000-04
5 ZhuangZhaoxia Lin Zhiyong et al. The affiliated Stomatologic hospital of Shandong Medical University. Jinan (250012);Study of Chitosan Membrane on Biodegra-dation and Tissue Regeneration[J];Chinese Journal of Dental Materials and Devices;1997-04
6 DUN Jie-ning, DENG Shu-hai, MIAO Cai-yun, ZHANG Na (Deptartent of Pharmaceutics, College of Pharmacy, Shandong University, Jinan 250012, Shandong, China);Studies on lung-targeting DATS-loaded chitosan microspheres[J];Journal of Shandong Univenity(Health Scicnes);2005-05
7 Wang Aiqin, Yan Zhihong, Jia fiaoquan, Cao Long, Xue ZuoliangLanzhou Institute of Chemical Physics, Chinese Academy of Sciences (Lanzhou 730000);Preparation and Quality Analysis of N-(carboxymethyl) chitosan[J];Chinese Journal of Biochemical Pharmaceutics;1996-04
8 ZHANG Chen, WANG Zhe-sheng, GAO Jing-heng. (The First Clinical Collage, China Medical University, Shenyang 110001, China);The biological properties of acellular cartilage[J];Journal of Practical Aesthetic and Plastic Surgery;2003-02
9 Cao Zongshun;Lu Fengqi;Wang Fengshan;Nie Zhengui(Dept. of Pharmacy,Shandong Medical University,Jinan 250012);Degradability of Chitosan Films[J];Journal of Biomedical Engineering;1995-04
10 SONG Ai-mei, YANG Pi-shan, SUN Qin-feng (Faculty of Stomatology, Shandong Medical University, Jinan 250012, China);The experimental study on relationship between time for placement of a barrier membrane and periodontal regeneration in dogs[J];Chinese Journal of Conservative Dentistry;2001-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved