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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2009-28
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Effects of basic fibroblast growth factor transfection on canine gingival fibroblasts

Chen Xin-jian1,Yan Fu-hua1,Zhong Quan1,Zhao Xin1,Jiang Yi-ping2 1Stomatological Hospital Affiliated to Fujian Medical University,Fuzhou 350002,Fujian Province,China; 2Research Center of Cells and Developmental Engineering,Fujian Medical University, Fuzhou 350002, Fujian Province, China  
BACKGROUND:Studies have demonstrated that exogenous basic fibroblast growth factor (bFGF) has intensive effects to promote proliferation of gingival fibroblasts (GFs) cultured in vitro and the healing of gingival wounds. OBJECTIVE:To investigate the effects of bFGF gene transfection on the biological performance of Beagle canine GFs. DESIGN,TIME AND SETTING:An observation and comparison in vitro experiment regarding cells was accomplished in Centre of Cell Biology and Development of Fujian Medical University and Department of Comparative Medicine in Fuzhou General Hospital of Nanjing Military Area Command of Chinese PLA from April to September of 2008. MATERIALS:Four beagle dogs,male,12 months old,weighing 10-13 kg were used in this experiment. pIRES2-EGFP-bFGF plasmid containing full-length human bFGF gene cDNA was constructed and conserved by our institution. METHODS:Free gingiva of the 2nd,3rd and 4th premolars were excised from left upper jaw of Beagle dogs,rinsed with aseptic phosphate buffer four times,then cut into pieces and digested with 2.5 g/L pancreatin for 2 hours at 37 ℃. After the centrifugation and supernatant removal,DMEM containing 10% fetal bovine serum was added to incubate on 6-well plate with coverlips in 5% CO2 incubator at 37 ℃. Logarithmically growing cells were digested and passaged. GFs were transfected with pIRES2-EGFP-bFGF plasmid using liposome mediated method,while vacant plasmid transfection and un-transfection group served as controls. MAIN OUTCOME MEASURES:Proliferation and apoptosis feature of the GFs were evaluated by MTT and AOEB,respectively. The activity of alkaline phosphatase was assayed by chemical colorimetry. RESULTS:All of three groups cells entered log phrase on three days after transfection. MTT results showed that the proliferation of GFs transfected with bFGF was greater than cells transfected with vacant vector and untransfected cells (P 0.05). AO/EB dyeing showed the apoptosis rate of GFs transfected with bFGF was reduced compared with other two groups (P 0.05). After bGFG gene transfection,the ALP activity remained unchanged and there was no significant difference compared with untransfected cells. CONCLUSION:The transfection of bFGF gene to GFs can promote the proliferation of GFs and depress the apoptosis. No promotion is present with regard to the GFs differentiation.
【Fund】: 国家自然科学基金资助项目(34071892);; 福建省科技厅、福建医科大学科学研究发展基金资助项目(XZ04011)~~
【CateGory Index】: R780.2
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Chinese Journal Full-text Database 10 Hits
1 YANG Pi-shan+1, SUN Qin-feng+1, SONG Ai-mei+2,GE Shao-hua+1.(1.Dept. of Periodontology, Affiliated Stomatological Hospital;2.Dept. of Dentistry, The Second Hospital, Shandong University, Jinan 250012,China);The Effect of bFGF on Proliferation of Periodontal Fibroblast-like Cells of Dogs[J];West China Journal of Stomatology;2004-01
2 TANZhen, ZHAO Qing, GONG Ping. ( The Colledge ofStomatology, Sichuan University, Sichuan, 610041,China);The influence of bFGF on tbe biological characteristics of osteoblasts and periodontal ligament fibroblasts[J];Chinese Journal of Prosthodontics;2005-03
3 Li Shu, Wang Shuozhi, Fan Mingwen, et al. Stomatological College & Hospital of Shandong University Jinan 250012, China;A comparative study of human gingival fibroblasts and periodontal ligament cells in their mineral characteristics in vitro[J];Journal of Clinical Stomatology;2002-05
4 JI Jian-xin, LIAO Wei-jiao, QIU Zhi-hui, et al. (The first Affiliated Hospital of Guangzhou Medical College, Guangzhou 510120, Guangdong, P. R. China);The impact of basic fibroblast growth factor or insulin-like growth factor-1 on growth of proliferation of periodontal ligament cells on root surface[J];China Tropical Medicine;2006-11
5 NIE Rui,WU Yun-xia(Dept of Stomatology,First Clinical Medical College,Shanxi Medical University,Taiyuan 030001,China);Effects of PDGF-BB and bFGF on ALP activity in human periodontal ligament cells:an in vitro study[J];Journal of Shanxi Medical University;2008-03
6 Duan Kaiwen,Zhang Mingzhu, Zhang Lu,et al.Department of Stomatology,The First Affiliated Hospital of Kunming Medical College,Kunming 650032, China;The effect of basic fibroblast growth factor and chitosan on human periodontal ligament fibroblasts[J];Journal of Practical Stomatology;2005-04
7 Zhong Quan1,Yan Fu-hua2,Li Yan-fen2,Zhao Xin31College of Stomatology,Fujian Medical University,Fuzhou 350004,Fujian Province,China;2Department of Periodontology,3Central Laboratory,Affiliated Stomatological Hospital of Fujian Medical University,Fuzhou 350002,Fujian Province,China;Isolation and culture of gingival fibroblasts of Beagle dogs in vitro[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2008-50
8 WU Yong,WU Zhi-fen,WANG Qin-tao, et al ( College of Stomatology,The Fourth Military Medical University, Xi'an 710032,China );The combining effects of recombinant human bone morphogenetic protein-2 and basic fibroblast growth factor on the proliferation of human periodontal ligament cells[J];Chinese Journal of Conservative Dentistry;2001-01
9 WANG Kun,XU Yan-zhi,ZHU Meng-meng(Deparment of Stomatology,Fourth Hospital of Hebei Medical University,Shijiazhuang 050011,China);Study of biological activity of gingival fibroblasts and periodontal ligament cells[J];Chinese Journal of Conservative Dentistry;2008-06
10 LIU Zhao-zhe~1,CHEN Jie-peng~2,ZHAO Su-lan~2,LI Chang-ling~(1*)(1.School of Pharmaceutical Sciences,Peking University Health Science Center,Beijing 100083,China;2.Strand Biotechnology Institute of Research,Shantou 515041,China);Apoptosis-inducing effect of alternol on mouse lymphocyte leukemia cells and its mechanism[J];Acta Pharmaceutica Sinica;2007-12
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WT5”BZ] LIU Xiao yong et al.Faculty of Stomatology,Capital University of Medical Science,Beijing 100050 [WT5”HZ];EFFECTS OF BASIC FIBROBLAST GROWTH FACTOR ON THE PROLIFERATION AND ALKALINE PHOSPHATASE EXPRESSION OF HUMAN PERIODONTAL LIGAMENT CELLS.[J];Beijing Journal of Stomatology;2001-02
2 GE Shao hua et al Dept.of Stomatology,Shandong University,Jinan,250012;CULTURE,MORPHOLOGY AND PROLIFERATION OF THREE TYPES OF HUMAN PERIODONTAL CELLS[J];Beijing Journal of Stomatology;2003-02
3 LIU Hong1, WU Zhi Feng 2, WANG Qin Tao 2, ZHOU Bin 3 (1. Department of Stomatology, Changhai Hospital, Second Military Medical University, Shanghai 200433, China; 2. Department of Periodontology, School of Stomatology, Fourth Military Medical Univers;Effect of insulin-like growth factor-Ⅰ on osteocalcin secretion of periodontal ligament cells[J];Academic Journal of Second Military Medical University;2001-05
4 SHEN Hong Lei 1,WU Jun Zheng 2,ZHANG Dong Yin 1,LI Feng 2,ZHOU Jun Ling 1,LIU Xiao Ming 1 1Department of Surgery,The 23rd Hospital of Chinese PLA,Urumqi 830006, 2Department of Oral Biology,Stomatological College,Fourth Military Medical;Bone repair of radial defects with bFGF/FB composition in rabbits[J];JOURNAL OF THE FOURTH MILITARY MEDICAL UNIVERSITY;2000-04
5 ZHENG Lei WANG Qian WEI Kuan-hai Pei Guo-xian (Department of Orthopedics and Traumatology. Clinical Laboratory Nanfang Hospital, First Military Medical University. Guangzhou 510515. China);Biological effects of basic fibroblast growth factor on bone marrow stromal cells[J];JOURNAL OF FIRST MILITARY MEDICAL UNIVERSITY;2000-02
6 Fan Weihua, et al. Guang Dong Provincial Stomatological Hospital, Guangzhou 510280;Effect of Acid and Inacid Root Conditioning Agents on Root Surfaces.Ⅰ Ultrastructure Observation.[J];Journal of Dental Prevention and Treatment;2003-02
7 LI Shu,WANG Shuo zhi,FAN Ming wen,et al.Stomatological College of Stomatology, Wuhan University,Wuhan 430079 [;Characterization of fibroblasts derived from human periodontal ligament and gingiva-a study of cell morphology、growth curve and activity of alkaline phosphatase.[J];Journal of Comprehensive Stomatology;2001-02
8 Li Shu, Wang Shuozhi, Fan Mingwen, et al. Stomatological College & Hospital of Shandong University Jinan 250012, China;A comparative study of human gingival fibroblasts and periodontal ligament cells in their mineral characteristics in vitro[J];Journal of Clinical Stomatology;2002-05
9 ZHANG Jian-nan,ZHANG Yan,REN Bing-xu,et al.Department of Anesthesiology,Affiliated Fourth Hospital,Soochow University,Wuxi 214062,China;Effect of ketamine on ERK expression in hippocampal neural cells and the ability of learning behavior in minor rats[J];The Journal of Clinical Anesthesiology;2007-12
10 JI Jian-xin, LIAO Wei-jiao, QIU Zhi-hui, PAN Guang-ci, ZHANG Ting (Department of Stomatology, First Affiliated Hospital, Guangzhou Medical College , Guangzhou 510120, China );Effect of Platelet-Derived Growth Factor-BB and Epidermal Growth Fact or on the Proliferation, Fibronectin and Alkaline Phosphatase Activity of Human Periodontal Ligament Cells[J];Journal of Tropical Medicine;2005-03
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