Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Clinical Rehabilitative Tissue Engineering Research》 2009-53
Add to Favorite Get Latest Update

Establishment of focal segmental glomerulosclerosis models induced by nephrectomy combined with adriamycin in rabbits

Xu Fan1,Pan Xing-hua1,Feng Fan-zhe2,Li Xiao1,Yang Ren-hua1,Li Zi-an11Center for Stem Cell Biology and Tissue Engineering,Kunming General Hospital of Chinese PLA,Clinical College of Kunming General Hospital of Chinese PLA,Kunming 650032,Yunnan Province,China;2Brigade for Cadets,Third Military Medical University of Chinese PLA,Chongqing 400030,China  
BACKGROUND:Rat models induced by unilateral nephrectomy combined with adriamycin are widely used in screening anti-drugs of glomerular sclerosis.However,few reports concerning this method on inducing rabbit model of glomerular sclerosis OBJECTIVE:To establish a rabbit model of glomerular sclerosis,and to observe the renal function and histopathological changes during model preparation.DESIGN,TIME AND SETTING:Randomized controlled animal experiment was performed in Laboratory Animal Center of Kunming General Hospital of Chengdu Military Region between December 2008 and May 2009.MATERIALS:Twenty-five Japanese big-ear rabbits,weighing 1.75-2.25 kg,half males and half females,were selected to establish glomerular sclerosis models,and randomly divided into normal (n=10) and model (n=15) groups.METHODS:The left kidney of rabbits in the model group was removed under anesthetized with 30g/L saline solution of sodium pentobarbital (1 mL/kg).Rabbits in the normal group underwent a similar surgical procedure without kidney removing.At 1 week after operation,5 mg/kg adriamycin was injected into rabbits in the model group,3 mg/kg adriamycin was reinjected 2 weeks later.Same volume of physiological saline was injected in the normal group.MAIN OUTCOME MEASURES:The renal blood biochemical indexes were detected prior to and at weeks 4,6,and 8 after model preparation.One nephridial tissue was harvested from each group to undergo pathological observation at week 8 after the second medication.RESULTS:①Kidney in the normal group presented slightly white,slightly tough texture with smooth surface.②Pathological features of focal segmental glomerulosclerosis was showed in the model group under a light microscope,presented as extracellular matrix hyperplasia,mesangial region expansion,renal glomerular capillary wall ball mix adhesion,tubule degeneration,or even interstitial fibrosis,as well as interstitial infiltration of inflammatory cells.③Compared to the normal group,the total protein and albumin was and decreased,triglyceride,total cholesterol,low density lipoprotein cholesterol,urea nitrogen,and creatinine were significantly increased in the model group.The urine protein content of rabbits in the model group was obviously increased at 4 weeks,gradually reached a platform after 8 weeks,which still greater than that of the normal group.④SPECT showed that the glomerular filtration rate of the model group was notably decreased (33 mL/min) than the normal group (92.6 mL/min).CONCLUSION:The unilateral nephrectomy combined with adriamycin in rabbits results in the formation of glomerular sclerosis,renal function and 24 h urinary protein is more visible in the phase change.
【CateGory Index】: R-332;R692.6
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 4 Hits
1 ZHAO Hong-wen,YU Rong-jie,PENG Kan-fu,et al.(Department of Kidney,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Ultrastructural observation of adriamycin induced focal segmental glomerularsclerosis in rats[J];Chongqing Medicine;2008-08
2 ZHAO Hong-wen,YU Rong-jie,LIU Hong,PENG Kan-fu,WU Xiong-fei(Department of Kidney,Southwest Hospital,Third Military Medical University,Chongqing 400038,China);Endothelial progenitor cells transplantation ameliorates the progression of progressive focal segmental glomerular sclerosis[J];Acta Academiae Medicinae Militaris Tertiae;2007-21
3 Jin Shi-lan (The Affiliated Hospital of Yanbian University,Yanji 133000,China);Valsartan Prevent Glomerular Sclerosis in Remnant Kidney Model[J];Chinese Journal of Medicinal Guide;2008-02
4 ZHENG Donghui*,XING Changying,CHEN Juhua,et al.*The Sencond People's Hospital of Huai'an City,Jiangsu Province 223002;Effects of Mycophenolate Mofetil on Focal Segmental Glomerulosclerosis[J];The Journal of Medical Theory and Practice;2006-11
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 Li wei,Li Ai-hua (Department of Nephrology,The People's Hospital of Xinyu of Jiangxi,Xinyu 338025,China);Therapeutic Effect of 30 Cases on the Transfusion Methods of Cyclophosphamide in the Treatment of Idiopathic Nephritic Syndrome[J];Chinese Journal of Medicinal Guide;2008-09
2 LIU Aimin1 HUANG Xionliang2 XIE Lingling2 ZHANG Wei2 SU Xiaoru2The First People's Hospital of Zhaoqing, Zhaoqing, 526021 China. Animal Centre Lab ofGuangzhou University of TCM, Guangzhou, 510405 China;Treatment of Acute Urethra Infection by Therapy, TCM Combined with Western Medicine (50 Cases)[J];International Medicine & Health Guidance News;2004-18
3 MA Ming-ling(Guangdong Nanxiong People'S Hospital,Nanxiong,Guangdong,512400);Observations on the Curative Effect of Chinese Native Medicine Sausage Treatment on 95 Cases of Chronic Renal Failure[J];Journal of Gannan Medical University;2007-02
4 YU Yi,XIE Fu-an,WANG Yan(Department of Blood Purifi cation,Fuzhou General Hospital of Nanjing Military Command,Fuzhou 350025,China);Transplantation of bone marrow derived endothelial progenitor cells facilitates angiogenesis after renal ischemia/reperfusion injury[J];World Clinical Drugs;2009-02
5 LUO Youlin,JIANG Liuzhou. Shajing People's Hospital of SHenzhen City,Shenzhen 518104,Guangdong,P.R.CHINA;Clinical characteristics of 26 patients with post-traumatic acute renal failure[J];Hainan Medical Journal;2009-02
6 ZHOU Fengwei,FANG Nuo,LI Yaxiu Department of Nephrology,Shijiazhuang Pingan Hospital,Hebei,Shijiazhuang 050021;Effect of Qidanyishenjingtang pill on diabetic nephropathy[J];Hebei Journal of Traditional Chinese Medicine;2009-05
7 HAN Xiao-wei CHENG Shu-bi QI Ai-rong LI Shun-min YI Tie-gang ZHENG Yi-hou Shenzhen Affiliated Hospital,Guangzhou University of Traditional Chinese Medicine,Shenzhen 518033,China;Clinical research of the Jianpiyishenfang decoction with acupuncture and moxibustion with the renal osteodystrophy disease in maintenance hemodialysis[J];Chinese Journal of Modern Nursing;2008-11
8 ZHANG Qingyun;Application of Humanistic Nursing Care in Patients with Chronic Globular Nephritis[J];Journal of Nursing Science;2008-17
9 Lu Zhanfeng,Guo Xianting Xuchang No.2 People's Hospital, Henan;Combined Traditional Chinese and Western Medicine for HSPH[J];Journal of Henan College of Traditional Chinese Medicine;2005-03
10 Chen LanyunThe Traditional Chinese Medicine Hospital of Henan Province,Zhengzhou,Henan,China,450002;A Clinical Observation on Treatment of Chronic Renal Failure with Chuanxiongzine Injection and Huangqi Injection[J];Journal of Henan University of Chinese Medicine;2006-01
China Proceedings of conference Full-text Database 2 Hits
1 LIUWen-jing,WEI Lian-bo,LONGHai-bo (Department of Nephoropathy of Western and Chinese Mdicine,Zhujiang Hospital,Southern Medical University,Guangzhou,510282,China);Effect of Shenkang Pill on the chronic glomerulonephrititis[A];[C];2009
2 Zhu pijiang,Zhou xun,Zhao hua,et al.Kidney Disease Institute of Integrated Traditional Chinese and Western Medicine of Zhenjiang,Zhenjiang 212028 China;Curative Effect Observation of Qinglijieduhuoxietang Combined With Western Medicine Treatment 45 Example of Refractory Nephrotic Syndrome[A];[C];2009
【Secondary Citations】
Chinese Journal Full-text Database 4 Hits
1 LIU Shui-bing, TIAN Qiong. Teaching Experiment Center, the Fourth Military Medical University, Xi' an Shanxi 710032, China;Preparation of Mouse Sex - determining Gene, SRY Probe[J];Military Medical Journal of South China;2005-03
2 ZHANG Lifen,HUANG Wenzheng,ZHU Xiaodi,et alDepartment of Nephrology Endocrinopathy,Dongzhimen Hospital of Beijing Chinese Medicine University,Beijing (100700);The Study on Adriamycin-induced Glomerulosclerosis Rats Model[J];Chinese Journal of Integrated Traditional and Western Nephrology;2005-04
3 LIU Shaojun, GU YongYANG Haichun, et al. Division of Nephrology, Huashan Hospital, Fudan University, Shanghai 200040, China;Nephroprotective effect of mycophenolate mofetil on rat remnant kidney[J];Chinese Journal of Nephrology;2002-03
4 DONG Wei , GAI Lu-yue,JIN Hai-jie, GU Zheng, SONG Xin , ZHANG Miaa. Department of Cardiology, PLA General Hospital, Beijing 100853, China;Endothelial progenitor cells promote the vasculogenesis of ischemic limbs[J];Chinese Journal of Cardiology;2004-12
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved