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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2010-03
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Biomimetic synthesis and cytocompatibility of agar-hydroxyapatite composites

Cai Hua-qiong1,Li Quan-li 1,2,Zhou Jian 1,2,Tang Jian 3,Chen Hui 4 1College of Stomatology,Anhui Medical University,Hefei 230032,Anhui Province,China;2Provincial Laboratory of the Prevention and Treatment for Oral Diseases,Anhui Medical University,Hefei 230032,Anhui Province,China;3First Affiliated Clinical College of Anhui Medical University,Hefei 230032,Anhui Province,China;4China National Logging Corporation,Beijing 100000,China  
BACKGROUND:Studies regarding synthesizing composite bone repair materials with organic macromolecules as mineralizers template are the hot research spot.However,reports concerning using agar as bone repair materials are few.OBJECTIVE:To synthesize a novel agar-hydroxyapatite composite material,and to evaluate its physical and chemical properties as well as cytocompatibility.METHODS:①A certain amount of the non-nano-hydroxyapatite in hydrochloric acid solution was added into a certain amount of agar sol,and the reaction system was adjusted with PH value of 7-8.And then the precipitate was lyophilized to obtain the composite of agar-hydroxyapatite.②The third generation of rat bone marrow stromal cell(BMSC)was co-cultured with agar-hydroxyapatite.And the growth of cells was observed at days 1,3 and 5 after culture.RESULTS AND CONCLUSION:The results of X-ray diffraction patterns,Fourier transform infrared spectrometer(FTIR),thermal analyzer,transmission electron microscope,and scanning electron microscope showed that agar could manipulate the growth of hydroxyapatite,and hydroxyapatite nanocrystals was equably formed on the agar-fibers with high porosity.The BMSC grew well in the composite and form a clear cytoskeletal at days 3 and 5 after culture.The results reveal that agar-hydroxyapatite composite has good physical and chemical properties and cytocompatibility.
【CateGory Index】: R318.08
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