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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2010-23
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Immunophenotype determination of rat adipose tissue-derived mesenchymal stem cells at passage 4

Li Dong-fei,Yang Chun,Li Zhen,Dai Jing-xing,Yuan LinDepartment of Human Anatomy,South Medical University,Guangzhou 510515,Guangdong Province,China  
BACKGROUND:Many studies have confirmed that adipose tissue-derived mesenchymal stem cells (ADMSCs) can be collected using enzyme digestion method.At passage 10,ADMSCs grew well,but their surface marker expression decreases.OBJECTIVE:To isolate and cultivate rat ADMSCs and detect immunophenotype at passage 4.METHODS:ADMSCs of six Sprague-Dawley rats from inguinal fat pads were isolated and cultured.Adherent cells were amplified till passage 4.Morphology of ADMSCs was observed under a phase contrast microscope.The immunophenotype markers including CD11b,CD29,CD45,CD49d,CD90 and CD106 of ADMSCs were determined using flow cytometry.RESULTS AND CONCLUSION:Rat ADMSCs showed fibroblastic-like morphologic characteristics.The immunophenotype analysis revealed that the rat ADMSCs were positive for CD29 and CD90,but negative for CD11b,CD45,CD49d and CD106.The rat ADMSCs can be obtained through enzyme-based isolation procedures.The results revealed that the immunophenotype characterization of rat ADMSCs is consistent with mesenchymal stem cells.
【Fund】: 国家重点基础研究计划(973计划)项目(2007CB512705);; 国家自然科学基金青年科学基金项目(30801464)资助~~
【CateGory Index】: R329
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Chinese Journal Full-text Database 2 Hits
1 Li Hong-mian1,2, Gao Jian-hua2, Lu Feng2, Li Hua2, Chen Xiao-wei2, Fu Bing-chuan21Department of Plastic and Aesthetic Surgery, Affiliated Zhongshan Hospital of Sun Yat-sen University, Zhongshan 528403, Guangdong Province, China; 2Department of Plastic Surgery, Nanfang Hospital of Southern Medical University, Guangzhou 510515, Guangdong Province, China;Biocompatibility of type Ⅰ sponge collagen with rabbit’s adipose-derived stem cells in vitro[J];Journal of Clinical Rehabilitative Tissue Engineering Research;2009-25
2 Song Kedong,Guo Wenhua,Liu Tianqing,Ning Ruiming,Bao Chunyun,Ma Xuehu(Dalian R&D Center for Stem Cell and tissue Engineering,State Key Laboratory of Fine Chemicals,Dalian University of Technology,Dalian,Liaoning 116024,China);Investigation of co-culture of human adipose-derived stem cells and mature adipocytes[J];Sciencepaper Online;2010-06
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