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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2011-01
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Vascular endothelial cell growth factor combined with bone morphogenetic protein-2-modified bone marrow mesenchymal stem cells in repair of femoral head necrosis

Cui Da-ping1,Zhao De-wei1,2 1Dalian University of Technology,Dalian 116023,Liaoning Province,China; 2Department of Orthopaedics,Zhongshan Hospital of Dalian University,Dalian 116001,Liaoning Province,China  
BACKGROUND:Vascular endothelial cell growth factor (VEGF) combined with bone morphogenetic protein (BMP) was used to repair necrotic bone,which can maintain the osteogenic phenotype of seed cells,and effectively secrete VEGF and BMP-2,and effectively promote blood vessel regeneration and contribute to formation and revascularization of tissue engineered bone tissues. OBJECTIVE:To observe the therapeutic effect on the treatment of avascular necrosis of the femoral head by using bone marrow mesenchymal stem cells (BMSCs) modified by VEGF-165 and BMP-2 in vitro. METHODS:The model of avascular necrosis of rabbit femoral head on right leg. There were single core decompression group,core decompression and BMSCs group,decompression with VEGF-165/BMP-2/BMSCs group. Necrotic bone was cleared out on arthroscope. Osteogenesis and angiogenesis were inspected by histology. RESULTS AND CONCLUSION:Arthroscope observation demonstrated that necrotic bone was cleared out in each group,and fresh blood was flowed out. Histomorphology determination showed that blood vessel number and new bone area in the repair region were significantly greater at various time points following transplantation in the decompression with VEGF-165/BMP-2/BMSCs group compared with single core decompression group and core decompression and BMSCs group (P 0.05). These suggested that VEGF-165/BMP-2 gene transfection strengthened osteogenic effects of BMSCs,elevated number and quality of new bones and accelerated the repair of avascular necrosis of the femoral head.
【Fund】: 国家自然科学基金项目(30471752) 骨髓基质干细胞体外转导修饰关节镜监视下自体回植治疗股骨头缺血性坏死的研究~~
【CateGory Index】: R681.8
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