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《Journal of Clinical Rehabilitative Tissue Engineering Research》 2011-15
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Effect of different-intensity static magnetic field on the reorganization of osteoblast cytoskeleton

Wang Sheng-guo1,Zhou Li2,Chen Yang-xi2 1Department of Stomatology,the Second Affiliated Hospital of Chongqing Medical University,Chongqing 400010,China;2Department of Orthodontics,West China College of Stomatology,Sichuan University,Chengdu 610041,Sichuan Province,China  
BACKGROUND:Mechanisms addressing effects of static magnetic field on osteoblast proliferation,cytokine secretion and alkaline phosphatase(ALP) activity remain poorly understood.OBJECTIVE:To evaluate the effects of static magnetic field loading the cytoskeleton reorganization and cytoskeletal protein expression.METHODS:The osteoblasts were harvested from skull of SD rats within 24 hours after born and cultured.Static magnetic fields with 0,8,50 and 160 mT were added on osteoblasts.The BODYPY-Phaloidi was used to stain cytoskeleton,and fluorescence intensity was measured by laser scanning confocal microscope and image J at 24,48 and 72 hours after loading.RESULTS AND CONCLUSION:At 24,48 and 72 hours after culturing under 8,50 and 160 mT static magnetic fields,the cytoskeleton fluorescence concentrated to the nucleus and the fluorescence intensity up-regulated(P 0.05),especially strongest in the 50 mT static magnetic field.The static magnetic field can result in the reorganization of the osteoblast cytoskeleton.
【CateGory Index】: R783.5
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