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《Chinese Journal of Tissue Engineering Research》 2016-06
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Bone marrow mesenchymal stem cell transplantation protects against intestinal ischemia-reperfusion injury in rats

Liu Hong-feng;Li Lu;First Affiliated Hospital of Nanyang Medical College;Second Affiliated Hospital of Zhengzhou University;  
BACKGROUND: Bone marrow mesenchymal stem cells have good proliferation and paracrine functions, which have irreplaceable advantages in the treatment of intestinal diseases. OBJECTIVE: To explore the effects of bone marrow mesenchymal stem cell transplantation on intestinal ischemia-reperfusion injury in rats. METHODS: Forty-eight Sprague-Dawley rats were enrolled to make animal models of ischemic reperfusion injury of the intestine, and then model rats were randomized into experimental and control groups. After modeling, 1 m L bone marrow mesenchymal stem cells or the same volume of normal saline were injected into the intestinal mucosa of rats in the two groups, respectively. At hours 0, 2, 6, 24, 72, 120 after injection, serum diamine oxidase, tumor necrosis factor α, and D-lactic acid levels were detected by ELISA method. At 24 hours after injection, rat intestinal tissues were taken and observed pathologically under light microscopy, and their close connections were observed under transmission electron microscope. ZO-1 protein levels were detected by immunohistochemistry method. RESULTS AND CONCLUSION: Compared with the control group, the serum diamine oxidase, tumor necrosis factor α, and D-lactic acid levels were significantly lower in the experimental group at hours 6 and 24 after injection(P 0.05). Intestinal necrosis, villous edema, intestinal congestion and inflammatory cell infiltration in the experimental group were milder than those in the control group. In addition, the ZO-1 protein expression in the experimental group was higher than that in the control group. Experimental results show that bone marrow mesenchymal stem cell transplantation into the intestinal mucosa can improve the intestinal mucosal permeability in rats with intestinal ischemia-reperfusion injury.
【Fund】: 河南省科技厅基础科技攻关课题(122300450037)~~
【CateGory Index】: R574.5
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