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《Journal of Modern Stomatology》 2010-04
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The influence of MCP-1 on the apoptosis of osteoblast precursors and the expression of apoptosis Caspase3 protein

WANG Yi,LI Songying.Quzhou Traditional Chinese Medicine Hospital,Quzhou 324002  
Objective To investigate the influence of MCP-1 on the apoptosis of osteoblast precursors and to evaluate the expression of apoptosis Caspase3 protein.Methods The expression of chemotactic factor MCP-1 in MC3T3-E1 was detected by immunofluorescence in vitro.Different concentrations of chemotactic factor MCP-1 were added in MC3T3-E1 cells,the apoptosis rate was detected by flow cytomertry and the expression of Caspase3 was checked by immunocytochemistry.Results The expression of MCP-1 was positive in MC3T3-E1.Flow cytomertry test declared that the apoptosis of MC3T3-E1 with serum culture medium was not more than 5%.Compared with normal serum group,the apoptosis rate of serum-free group increased significantly.In the range of(1~100)ng/mL MCP-1,the MCP-1 apoptosis was declined compared with the serum-free group(P0.05).Caspase3 was reduced on average gray values in MCP-1 group(P0.05).With the concentration of MCP-1(1ng/mL~100ng/ml) increased,the expression of Caspase3 was declined.Conclusion That MCP-1 regulated the expression of Caspase3 in MC3T3-E1 resisted possibly the apoptosis induced by serum-free,thus promoted osteogenesis.
【Fund】: 浙江省教育厅基金资助项目(N20090361)
【CateGory Index】: R780.2
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