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Determination of chlorogenic acid and baicalin in Shuanghuanglian injection by HPLC

Zhang Huanian(Zhang HN),Yang Shaofang(Yang SF),Chen Jiye(Chen JY),et al(Children′s Hospital of Wuhan,Wuhan 430016)  
OBJECTIVE:To develope a method for the determination of chlorogenic acid and baicalin in Shuanghuanglian injection,to contral its quality.METHOD:The colum was packed with ODS C 18 (150mm×4.6mm).Methanol water(pH 2.7) was used as mobile phase.Chlorogenic acid and baicalin were separated by HPLC with grade elution.RESULTS:The content of chlorogenic acid in Shuanghuanglian injection was 0.70%~0.75%(mg/mg),and that of baicalin was 10.25%~11.38%(mg/mg).CONCLUSION:The method was rapid convenient and accurate,and is suitable for the quality control of Shuanghuanglian injection.
【CateGory Index】: R927.2
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