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《Chinese Journal of Modern Applied Pharmacy》 2017-12
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Fabrication and Characterization of Peptide Modified Hyaluronic Acid Scaffolds Applied for the Growth of Bone Marrow Mesenchymal Stem Cells

HUANGFU Mingyi;LI Liming;LIU Huina;GUO Wangwei;GUO Ningning;HAN Min;GAO Jianqing;College of Pharmaceutical Sciences, Zhejiang University;  
OBJECTIVE To fabricate a hydrogel scaffold based on hyaluronic acid and modified with a laminin peptide for the growth of bone marrow mesenchymal stem cells(BMSCs). METHODS The scaffold was prepared by the crosslink between HA and adipic hydrazide and modified by a laminin peptide after freeze-drying method. FTIR and NMR spectroscopy were used to characterize the chemical structure of the scaffold. The surface morphology and internal structure of the scaffold were characterized by scanning electron microscopy(SEM) and mercury intrusion. The adhesion growth of BMSCs on the scaffolds was examined by confocal laser scanning microscope(CLSM). RESULTS The results of 1 H-NMR and FTIR showed that the scaffold was successfully cross-linked and modified by the peptide. SEM results showed that the scaffolds had a regular three-dimensional network structure. The porosity was 94.02% and the water swelling rate was 4 357.0%. CLSM results showed that BMSCs on the peptide-modified scaffold stretched out more pseudopodia and indicated the adhesion of the stent was significantly enhanced. CONCLUSION The peptide-modified hyaluronic acid scaffold showes a high porosity, a regular three-dimensional network structure, and significantly improve the adhesion of stem cells to scaffolds.
【Fund】: 国家自然科学基金资助项目(81102392)
【CateGory Index】: R318.08
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