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《Chinese Journal of Modern Applied Pharmacy》 2017-12
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Beneficial Effect of Stevenleaf on the Imbalance Between Th17 and Treg in Nonalcoholic Fatty Liver Disease Model Rats

RUAN Dan;LEI Jing;The Third People's Hospital of Hangzhou;  
OBJECTIVE To study the effect of stevenleaf on improving non-alcoholic fatty liver disease in rat model and clarify mechanism from the point of immune-regulation. METHODS Forty-eight of rats were divided into six groups: normal group, model group, Essentiale(150 mg·kg~(-1)), high-dose(240 mg·kg~(-1)), moderate-dose(120 mg·kg~(-1)) and low-dose of stevenleaf(60 mg·kg~(-1)) groups. Rats were given high fat food for continuous 10 weeks, then given correspond drugs for 8 weeks. The levels of serum AST and ALT were measured at 0, 4 and 8 weeks experimental session. The rats were anesthetized after administration 8 weeks. The peripheral blood lymphocytes and serum were separated to measure the contents of Th17 and Treg cells in peripheral blood by flow cytometry and the levels of IL~(-1)7, IL~(-1)0 and TNF-α in serum by enzyme-linked immunosorbent assay(ELISA). HE staining was used to observe the pathological changes of liver tissue and immunohistochemistry to observe the positive expression of IL~(-1)7 and Foxp3 in liver tissue. RESULTS The 240 mg·kg~(-1) of stevenleaf could reduce levels of serum ALT and AST after administration for 4 weeks(P0.01). stevenleaf 240 mg·kg~(-1), 120 mg·kg~(-1) and 60 mg·kg~(-1) could reduce levels of serum ALT and AST after administration for 8 weeks(P0.05, 0.01). Stevenleaf 240 mg·kg~(-1) and 120 mg·kg~(-1) could improve liver damage and reduce the level of TNF-α(P0.05). Stevenleaf 240 mg·kg~(-1) could reduce the content of IL~(-1)7 and increase IL~(-1)0 in serum(P0.05, 0.01), reduce content of Th17 cell(CD4+IL~(-1)7+) and increase CD4+CD25+ Treg in peripheral blood lymphocytes(P0.05), also significantly reduced the expression of inflammatory factor IL~(-1)7 and increased Foxp3 expression. CONCLUSION Stevenleaf could protect liver tissue damages, its mechanism may be related to regulating Treg/Th17 cell balance, reducing pro-inflammatory factors and increasing anti-inflammatory factors.
【Fund】: 浙江省中西医结合学会科研项目(2014LYZD001)
【CateGory Index】: R285.5
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