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《Modern Hospital》 2013-01
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THE CHANGES AND CLINICAL SIGNIFICANCE OF AQUAPORIN 5 AND CC16 IN LUNG TISSUE OF NEONATAL RATS WITH ACUTE LUNG INJURY

GUO Fang,SU Hang,ZHOU Huilian The Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,PRC  
Objective To discuss Aquaporin 5(AQP5)and CC16 change and clinical significance in early stage of acute lung injury in neonatal rats. Methods One hundred and forty newborn rats were randomized into two group(every group n=70),subgroup rats were randomized into normal saline pretreated group(NS group) and LPS group.Lung injury models in neonatal rats were established via intraperitoneal injection of 5 mg/kg lipopolysaccharides(LPS),The pathological changes were observed under microscopy,Wet/dry ratio of lung were examined.The expression of AQP5 was examined by Western Blot.Clara cell secretory protein(CC16) in serum and BALF was detected using enzyme-linked immunoadsordent assay(ELISA). Results ① Half-hour following LPS administration to neonatal rats,lung hemorrage was observed,these changes aggravated with time after LPS administration.Wet/dry ratio in neonatal rats at 2~4 hours after LPS were significantly increased,p0.01,then decreased.② 0.5 hour after injecting LPS,AQP5 level of lung began descending,it became the lowest after 1~2 hours,Significant differences were observed in AQP5 between the NS and LPS pretreated groups(p0.01).then the level became escalate and approached normal at 8 hours,aquaporin-5 level went down again to 24 hours(p0.01).③The contents of CC16 in BALF were significant lower after the injection of LPS,Significant differences were observed in the contents of CC16 in BALF between the NS and LPS pretreated groups(p0.01).The contents of CC16 in serum were higher level compared to NS group(p0.01),In LPS groups CC16 expression peaked at 4 h,8 and16 hours decreased. Conclusion ① The change of AQP5 reflect damage in type I epithelial cells.The dynamic changes of CC16 showed the Clara cells and the integrity of alveolar epithelium and vascular endothelial cell injury.These three is a sensitive marker reflecting in early stage of ALI.② The pront of time is difference in AQP5、CC16 in BALF and serum.In orther to early discover and intervent ALI,it is preferably to monitor the former two.③ Expresstion of AQP5 suggests therapeutic window period.
【Fund】: 广东省中医药局科研课题(编号:2010207)
【CateGory Index】: R722.1
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LUO Jiaohong,YIN Chunyan The second hospital of Guangdong province,Guangzhou,PRC,510000;ANALYSIS OF THE REASON OF 910 CASES OF PREMATURE DELIVERY AND PERINATAL OUTCOME[J];Modern Hospital;2009-12
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 MA Qiao-ya1,SUN Wan-sen2,REN Yan-yun2,WANG Zhu2 1Department of Geriatrics,2Department of Integrated Traditional Chinese and Western Medicine,Second Affiliated Hospital,Xi'an Jiaotong University College of Medicine,Xi'an 710004,China;Effects of Qufengtongluo recipe on proteinuria and glomerular filtration membrane in rats with adriamycin-induced nephropathy[J];Journal of Southern Medical University;2011-01
2 ZHOU Wang-mei,CHEN Dong-sheng,SANG Xian-fu,BAO Guang-xin,YU Chun-wen,HE Lin.Emergency department,Nanfang Medical University Nanfang Hospital,Guangzhou 510515,China;The study of alveolar fluid clearance in oleic acid-induced acute lung injury of rats[J];Guangdong Medical Journal;2009-07
3 WU Xili1,SUN Wansen1,YE Bingyu1,AN Peng1,SHI Xingmin2,FU Rongguo1,WANG Zhu1,QIAO Chenglin1 1Department of Integrated Traditional Chinese and Western Medicine,Second Affiliated Hospital,Xi'an Jiaotong University College of Medicine,Xi'an 710004,China;2Xi'an Jiaotong University College of Medicine,Xi'an 710061,China;Effects of Qufengtongluo Recipe on expressions of cell cycle regulatory proteins in rat mesangial cells[J];Journal of Southern Medical University;2012-12
4 YANG Miao*,LIU Yuyu,LI Shujuan.*Emergency Department,The First Hospital Affiliated to Hebei North College,Hebei,Zhangjiakou 075000,China;Experimental study on the improvement effect of Astragalus injection on the endotoxin-induced mesentery microcirculatory disturbance in rats[J];Hebei Medical Journal;2011-09
5 LIN Xiao-fei1,BEN Xiao-ming1,LI Juan2,GE Xu-hua1,CHEN Dong-mei1,YU Wen-liang1 (1.Department of Emergency,Nanjing Children′s Hospital Affiliated to Nanjing Medical University,Nanjing 210008,Jiangsu Province,China;2.Department of Postgraduate,Nanjing Medical University,Nanjing 210029,Jiangsu Province,China);Establishing Models of Piglets with Acute Lung Injury Induced by Oleic Acid[J];Journal of Applied Clinical Pediatrics;2011-20
6 YIN Jing1,YANG Li2(1.School of Clinical Medical Science,Southeast University,Nanjing 210009,China;2.Department of Pediatrics,Zhongda Hospital,Southeast University,Nanjing 210009,China);Expressions of MMP-2 and TIMP-2 in lipopolysaccharide-induced acute lung injury of neonatal rats[J];Modern Medical Journal;2009-03
7 HAN Xiao-juan1,MA Xue-qiang2,DU Yi-feng1(1.Department of Neurology,Provincial Hospital Affiliated to Shandong University,Jinan 250021,China; 2.The First Affiliated Hospital of Wenzhou Medical College,Wenzhou 325000,Zhejiang,China);Effects of Piper Kadsura Ohwi extracts on β-amyloid protein-induced activation of microglia in vitro[J];Journal of Shandong University(Health Sciences);2013-05
8 Wang Jing,Du Jiang,Zhou Xi-zhong,Liu Ru,Shen Wei,Wang Bin Department of Pediatrics,Zhujiang Hospital of Southern Medical University,Guangzhou 510282,Guangdong Province,China;Construction,expression and purification of a prokaryotic expression plasmid carrying pulmonary surfactant protein C[J];Chinese Journal of Tissue Engineering Research;2012-15
9 HAO Yan;ANALYSIS OF THE NEWBORN DEAD CAUSES FROM 2004 TO 2009[J];Modern Hospital;2010-05
10 GUO Fang,ZHOU Xiao-guang.The Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,China;Aquaporin 5 in lung tissue of neonatal rats with acute lung injury induced by LPS:its changes and significance[J];Chinese Journal of Neonatology;2010-04
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Wu Mei,Wang Wenqing,Wu Cuijiao, et al\ Department of Histology and Embryology, Qingdao Medical College, Qingdao 266021\;;THE INFLUENCE OF KADSURENONE UPON BLASTOCYST IMPLANTATION IN MICEIMMUNOHISTOCHEMICAL STUDIES[J];;1999-01
2 Department of Physical Chemistry Shen Chuanyong,et al;STUDY ON ANTIOXIDANT ACTIVITIES OF LIGNANS,KADSURENONE AND BURCHLLIN[J];JOURNAL OF BEIJING MEDICAL UNIVERSITY;1995-01
3 WANG Lin,YAO Jun,GENG Zhi-Min Department of Hepatobiliary Surgery,First Hospital,Xi'an Jiaotong University, Xi'an 710061,China;Role of kadsurenone in treatment of experimental acute pancreatitis in rats[J];Journal of the Fourth Military Medical University;2006-13
4 XU Hong-tao*,LI Hua-qiang,YU Jian,et al.*Department of Pediatrics,Wuhan General Hospital,Guangzhou Command,Wuhan Hubei 430070,China;Preliminary Study of Hyperoxia-induced Lung Injury in Neonatal Rats[J];Military Medical Journal of South China;2006-06
5 Guo Ruiyou,Zhang Su ming ,Fang Siyu et al.Department of Neurology,Tongji Hospital,Tongji Medical University,Wuhan 430030;Study on the protective effect of Piper kadsura Ohwi on the focal cerebral ischemia in aged rats.[J];CENTRAL CHINA MEDICAL JOURNAL;2000-04
6 Zhao Feng,Li Shengqing,Zhang Yufei,et al.Department of Respiratory,Xijing Hospital,Fourth Military Medical University,Xi'an 710032,China;Differentiation of bone marrow mesenchymal stem cells in injured rat lung[J];Medical Journal of Chinese People's Liberation Army;2007-02
7 ZHOU Xiao-guang(Department of Neonatology,Second Affiliated Hospital of Guangzhou Medical College,Guangzhou 510260,Guangdong,China);Diagnosis and management strategies of acute lung injury/acute respiratory distress syndrome[J];Journal of Clinical Pediatrics;2008-03
8 WANG Xue-Song , WANG Wei, RUAN Xu-Zhong Department of Neurology, Tongji Hospital, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China;The Study of Piper Kadsura Extract on Brain Protection after Cerebral Ischemia in Rats[J];Chinese Journal of Clinical Neurosciences;2003-01
9 ZHANG Xiong,WANG Wei,RUAN Xu zhong,et al (Department of Neurology,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China);Effects of kadsurenin mixture on platelet-activating factor and arachidonic acid metabolism in focal reversible ischemic brain area[J];Editorial Departmert of Chinese Journal of Geriatric Cardiovascular and Cerebrovascular Disease;2002-04
10 LI Su-hong(The Second People's Hospital of Yuhuan County,Yuhuan 317605,Zhejiang,China);Clinical Analysis of 100 Cases' Premature Birth[J];Journal of Liaoning University of Traditional Chinese Medicine;2008-06
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