Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Xiamen University(Natural Science)》 2005-05
Add to Favorite Get Latest Update

Purification and Some Characterization of Alkaline Phosphatase from Grass Carps(Ctenopharyngodon idellus)

ZHANG Ji-ping,LIN Jian-cheng,XIE Jin-jin,CHEN Qing-xi~*(Key Lab.of the Ministry of Education for Cell Biology and Tumor Cell Engineering,School of Life Sciences,Xiamen University,Xiamen 361005,China)  
An alkaline phosphatase was purified from Grass Carps(Ctenopharyngodon idellus) by following procedures:Tris-HCl buffer(pH 7.5) extraction,ammonium sulfate precipitation,ion-exchange chromatography on DEAE-32 cellulose,followed by gel filtration through SephadexG-200 and ion-exchange chromatography on DEAE-32.The final preparation was homogeneous on PAGE.The specific activity of the enzyme was 684 U/mg.The optimum pH is 10.41,and the optimum temperature is 27℃ for the hydrolysis of pNPP.Michaelis-Menten constant(K_m) is 1.01 mmol/L and the maximun velocity(V_m) is 5.49 mmol/L/min at pH 10.0 and 27℃.The enzyme is stable in the range of pH from 6.6 to 11.5 and in temperature below 45℃.A survey of effects of metal ions on the enzyme showed that the positive monovalent metal ions(Li~+,Na~+ and K~+) have no effect on the enzyme;positive bivalent metal ions(Mg~(2+),Mn~(2+) and Co~(2+)) activate the enzyme,Zn~(2+)inhibit the enzyme.The result indicates that different metal(ions) have different effect on the ALP.The study of ALP from Grass Carps(Ctenopharyngodon idellus) may give some help to Grass Carps culture.
【Fund】: 教育部跨世纪人才经费和厦门大学科技创新工程基金(XDKJCX20043001)
【CateGory Index】: Q55
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved