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《Journal of Xiamen University(Natural Science)》 2006-05
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Characteristic Study of Egg Laying Hormone and Its Decomposed Produces from Aplysia Egg with MALDI-TOF Mass Spectrometry Technology

FANG Cai-wang~(1,2),HUANG He-qing~(1,2*),YAN Li~1,CAI Zong-wei~(2,3)(1.School of Life Science,2.State Key Laboratory of Physical Chemistry of Solid Surface,College Chemistry & Chemical Engineering,Xiamen Univ.,Xiamen 361005,China;3.Department of Chemistry,Hong Kong Paptist Univ.,Kowloon Tong,Hong Kong,China)  
Aplysia is a kind of mollusk classing to be gastropod.The abundant drugs including hormones have been found in Aplysia egg.In order to understand the relation between Aplysia behavior and its hormones,the egg in Aplysia(Notarcus leachii cirrosus Stimpson) was selected to study the distribution and function of various hormones and peptides.The egg laying hormone(ELH) and its decomposed produces from the egg of NLCS were extracted by several organic solution and further separated by a combined off-line method of the extraction and the reversed phase-HPLC for analysis of molecular structures.The composition and structural characteristics of ELH,the decomposed produces of ELH,the dimer of acidic peptide were identified by MALDI-TOF mass spectrometry and Paws software.With reference to analytical methods as previously described,the experimental results show that the egg in NLCS not only contains abundance ELH and its decomposed produces,but also AP and its decomposed produces with dimeric molecular structure.In addition,we find that ELH is composed of AP and the decomposed peptides of ELH,indicating that AP play an important role in signal transmission in egg,which the physical function of AP differs from that of ELH in egg.It is well known that ELH in egg see after carrying out the complex physiological function because it enables decomposing various peptides with different molecular structures.
【Fund】: 国家自然科学基金(40276033);; 福建省自然科学基金(C0310006)资助
【CateGory Index】: Q57-3
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