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《Journal of Xiamen Univeristy(Natural Science)》 2007-02
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Mitochondrial COI Sequence Analysis of Labidocera euchaeta Habitated in Xiamen Waters

YANG Wei-di,LIN Yuan-shao*,CAO Wen-qing,FANG Lü-ping(Dept.of Oceanography,Xiamen University,Xiamen 361005,China)  
Labidocera euchaeta Giesbrecht is a dominant planktonic copepod of the coastal waters of China.The specimens in the present study were collected from Xiamen Waters in February of 2004.Mitochondrial COI gene fragment of Labidocera euchaeta was amplified via PCR,the PCR products were ligated into T-vector(Promega Co.),cloned and sequenced.MtCOI gene fragment sequences of three female individual were obtained.The sequence length was 709 of base pairs,and the average contents of A,T,C,G were 23%,37%,15% and 25%,respectively.Genetic identity,99.29%,was calculated by comparison of sequences of Labidocera euchaeta.MtCOI sequence variation sites were found as much as 15 loci and variation types were transversion.The sequences of Labidocera euchaeta collected from Xiamen waters and the sequences of Labidocera rotunda(accession no.AY145428),Calanopia thompsoni(accession no.AY145429),Pontellina plumata(accession no.AB206443),Pontellopsis regalis(accession no.AB206444),Pontellopsis tenuicauda(accession no.AB206445),Pontella rostraticauda(accession no.AB206446) from GenBank were compared and the result showed that the genetic identity was about 87.34% in the 625 base pair region.In the present study,the software programs DNAMAN and MEGA3.1 were used to make gene trees showing the systematic relationships of Labidocera euchaeta collected from Xiamen waters and Labidocera rotunda,Calanopia thompsoni,Pontellina plumata,Pontellopsis regalis,Pontellopsis tenuicauda,Pontella rostraticauda.
【CateGory Index】: Q953
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