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Construction and Clone of Hybrid-plasmid between Filamentous Cyanobacteria(Plectonema boryanum )and E.coli

Sun Lichun; Lou Shilin(Dept. of Biol.)  
Hybrid-plasmid from Hpa Ⅱ-digested ppb_1and AccⅠ-cleaved pUC13 was cloned into E. coli JM109. The purified hybrid-plasmid was transformed backwardly into freshly prepared spheroplast or filament from P. boryanum. The transformed algal colonies displayed anti-ampicillin activity in a level up to two times higher concentration of Ap had been confirmed in plate culture with an AP sensitific E. coli strain. Nevertheless,the acquired Ap ̄r can not withstand this level of AP on longer succesive culture.
【Fund】: 国家自然科学基金
【CateGory Index】: Q785
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