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《Journal of Xinjiang Agricultural University》 2006-02
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β-Casein Gene Expression in Different Mammary Epithelial Cells

HU Yan-qiu~(1,2),TAN Li-xin~1,LUO Shu-ping~2,NIU Zhi-gang~1,LI Wen-rong~1(1.Key Laboratory of Animal Biotechnology of Ministry of Agriculture,Xinjiang Academy of Animal Science,Urumqi,830000;2.College of Agronomy,Xinjiang Agricultural University,Urumqi,830052)  
Reverse transcription-polymerase chain reaction(RT-PCR)was used to investigate the expression of β-Casein gene in GMEC.The transcription of two mammary epithelial cell lines(C127 and T47D) was also analyzed to compare and detect the β-Casein gene expression in different mammary epithelial cell lines.The results showed that β-Casein gene expression can be detected in primary cultured goat mammary epithelial cells(GMEC-1) derived from lactating goat as well as passaged cells GMEC-3 with the presence of hormones including prolactin,insulin and hydrocortisone.However,the expressions of β-Casein gene cannot be detected in both C127 and T47D cell lines.
【Fund】: 新疆高技术研究发展计划项目(200311105)
【CateGory Index】: Q786
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