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《Journal of Xinjiang Agricultural University》 2009-04
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RAPD Polymorphism of Capparis spinosa L.Population from Xinjiang

SHI Qing-hua,LI Jie,WANG Xi-dong,ZHOU Gui-ling(Key Laboratory of Agricultural Biotechnology,Xinjiang Agricultural University,Urumqi 830052,China)  
The total DNAs were extracted by CTAB method from three different Capparis spinosa L.Population in Xinjiang and its polymorphism was studied based on RAPD method.The three primers,which were more stably amplified,were randomly selected from 93 primers.Total 25 RAPD fragments were amplified,13 of which were polymorphic.Each primer could generate 6 to 10 DNA fragments.The percentage of polymorphic loci was 52%.The results analyzed by DPS version software showed that Jaccard similarity coefficients ranged from 0.69 to 0.86,whose mean was 0.76,indicating that three the genetic variations existed among the three different Capparis spinosa L.Population.A dendrogram was established,based on Hier archical cluster analysis could divide three different Capparis spinosa L.Population cuhivars into 2 groups,in which there were smaller genetic relations.
【Key Words】: Capparis spinosa L. RAPD cluster analysis genetic polymorphism
【Fund】: 国家自然科学基金项目(30660011)
【CateGory Index】: S567.19
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