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《The Chinese Journal of Human Sexuality》 2013-03
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Influence of cyclophosphamide on mouse sperm DNA and the function of curcumin interventio

GONG Qinqin1,ZHANG Changjun2,ZHANG Ying2,DONG Yifei2,RU Xue1,2,WANG Xiaoning1,2 1 The Research Center of Reproduction Medicine,The People's Hospital of Shiyan, Hubei University of Medicine,Hubei 442000,China 2 Hubei University of Traditional Chinese Medicine,Hubei 430065,China  
Objectives: To explore the protective effect of curcumin against cyclophosphamide-induced sperm DNA damage in mice.Method: We divided adult Kunming mice into four groups randomly.Those are normal group,model group,low curcumin treatment group,high curcumin treatment group.As for the model group,low curcumin treatment group and high curcumin treatment group,we performed 40mg/kg intraperitoneal injecton of cyclophosphamide(cp) for five days.At the same time,we performed 100mg/kg curcumin suspension gavage to low curcumin treatment group and 200mg/kg to high curcumin treatment group every day.As for the normal group and model group,we performed the same amount of 0.5% sodium carboxymethyl cellulose gavage.The process lasted for 28 days.Then we observed the sperm hypotonic swelling rate.We detected superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),malondialdehyde(MDA),reactive oxygen species(ROS) content and mouse sperm DNA integrity.Result: Compared with the model group,the curcumin groups have significantly higher testicular tissue SOD and GSH-Px content(P0.05,P0.01),have significantly lower MDS and ROS(P0.05,P0.01).Acridine orange staining(AOT) showed that compared with the model group,the mouse sperm DNA integrity of the curcumin groups increased obviously and the increase was statistically significant(P0.01).Conclusion: Cyclophosphamide can damage the mouse sperm DNA integrity which leads the decreased sperm nuclear maturity rate.Curcumin can improve the oxidation of cyclophosphamide in mice sperm DNA,and improve sperm DNA integrity.
【CateGory Index】: R285.5
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