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《Acta Veterinaria et Zootechnica Sinica》 2007-04
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Elementary Study on Immortalization of Porcine Vascular Endothelial Cell by Telomerase Reverse Transcriptase Transfection

SU Zheng-yuan1,ZHANG Yan-ming1*,HONG Hai-xia1,LIU Jian-ling1,2(1.College of Animal Science and Technology,Northwest A & F University,Yangling 712100,China;2.College of Life Science,Northwest University,Xian 710069,China)  
To elongate the proliferation life-span of porcine arterial endothelial cell(PAEC) in vitro.The eukaryotic expression plasmid pCI-neo-hTERT was transfected into PAEC by lipofection.FactorⅧ related antigen and CD34 of PAEC were identified after transfection by immunohistochemical method;we also detected telomerase activity of PAEC after transfection by PCR-ELISA.The results showed that Factor Ⅷ related antigen and CD34 in PAEC was positive after transfection.The PAEC after transfection expressed the vascular endothelial cell-specific protein still;Telomerase activity in PAEC was higher by hTERT introduction,the cell proliferation life-span was elongated to 20 population doublings compared to control.Our research indicated that telomerase activity can be activated in PAEC,thus the cell proliferation life-span was elongated in vitro.
【Fund】: 国家自然科学基金项目(30471290);; 陕西省教育厅专项科研计划项目(05JK296)
【CateGory Index】: S858.28
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