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《Chinese Journal of Animal and Veterinary Sciences》 2007-09
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Construction of Pig IL-2 Eukaryotic Expression Plasmid pcDNA-IL-2 and Its Adjuvant Effect on pcDNA-PRRSV-SC2-ORF5 Gene Vaccine

Xininigen2,3,CHENG An-chun1,2,WANG Ming-shu1,2,CHEN Xi-wen1,2,DOU Wen-bo1,2,LI Xue-mei1,2,LIU Wu-mei1,2,ZHANG Ping-ying1,2,LIU Fei1,CHEN Xiao-yue1,2(1.Sichuan Key Laboratory of Animal Disease and Human Health,Ya'an 625014,China;2.Research Center of Avian Disease,College of Animal Science and Technology,Sichuan Agricultural University,Ya'an 625014,China;3.College of Animal Science and Veterinary Medicine,Inner Mongolian Agricultural University,Huhhot 010018,China)  
In this study,pig IL-2 eukaryotic expression plasmid pcDNA-IL-2 was constructed and its adjuvant effect was investigated.Four groups of pigs were immunized with sterilized water,empty vector pcDNA3.1(+),PRRSV-SC2-OFR5 gene eukaryotic expression plasmid pcDNA-PRRSV-SC2-ORF5 and a mixed solution of pcDNA-IL-2 and pcDNA-PRRSV-SC2-ORF5 respectively.Serum of pig in each group was collected at different time post inoculation,and antibody to PRRSV was measured by ELISA.Peripheral T lymphocytes were acquired from a small quantity of blood of each pig,and the activity of lymphocyte proliferation was detected by MTT methods after being cultured with RPMI1640 culture medium.Content of CD+4and CD+8 of peripheral T lymphocytes in each groups of pigs were tested by cytospectrometry.Results showed that the antibody and peripheral T lymphocytes level,content of CD+4,CD+8 cell of the pigs immunized with both pcDNA-IL-2 and pcDNA-PRRSV-SC2-ORF5 were significantly higher than that of the pigs immunized with pcDNA-PRRSV-SC2-ORF5 alone,indicate that plasmid pcDNA-IL-2 can be used as a adjuvant to enhance PRRS gene vaccine.
【Fund】: 教育部“新世纪优秀人才支持计划项目(NCET-04-0906);; 四川省基础研究重大项目(05JY029-109);; 四川省“十一五”生物技术重大项目(2006Z05-03-01);; 四川省重点建设学科项目(SZD0418)
【CateGory Index】: S852.5
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