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《Chinese Journal of Animal and Veterinary Sciences》 2010-07
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Isolation and Identification of Endophytic Fungus from Oxytropis deflexa

LU Hao1,MA Yao1,ZHAO Bao-yu1,LAI Hang-xian2,LI Rong1,LU Wei1,RONG Jie1(1.College of Veterinary Medicine,Northwest A&F University,Yangling 712100,China;2.College of Resources and Environment,Northwest A&F University,Yangling 712100,China)  
To explore whether endophytic fungus exist in Oxytropis deflexa,the main locoweed species in Qinghai Province of China,endophytic fungus was isolated and cultured from O.deflexa by tissue separation,species and genus of endophytic fungus was identified by morphology and molecular biology.Three strains endophytic fungus were isolated and obtained from O.deflexa,and named EFJ-3,EFJ-4 and EFG-8,respectively.Morphology evidence and sequence analysis of the ITS region suggested that EFJ-3 was Fusarium proliferatum,EFJ-4 was Emericella rugulosa,EFG-8 was Aspergillus ustus.The result showed that endophytic fungal existed in O.deflexa locoweeds in Qinghai Province.
【Fund】: 西北农林科技大学人才基金项目(Z111020828);西北农林科技大学动物医学院院长基金项目
【CateGory Index】: S452
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