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《Chinese Journal of Animal and Veterinary Sciences》 2014-02
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Comparison of Indirect ELISA and Competitive ELISA for Detecting Peste des Petits Ruminants Virus Antibody

WANG Jing;LI Gang;SHI Li-jun;Hebei North University;Institute of Animal Science,Chinese Academy of Agricultural Sciences;  
The indirect ELISA and the competitive ELISA method were compared in the detection of serum antibody against Peste des Petits Ruminants Virus(PPRV).The PPRV N protein to be used as a coating antigen was expressed in BL21and purified by Ni Sepharose FF column.Ninetyfour sheep and twenty-five goats serum samples were detected by the ELISA methods based on the recombinant N protein.The coincidence rate of the indirect ELISA and the competitive ELISA kit was 74.8%,the coincidence rate of the competitive ELISA and the competitive ELISA kit was 96.6%.The detection results showed that the competitive ELISA is more reliable than the indirect ELISA method,and the competitive ELISA is suitable for clinical application.
【Fund】: 国家自然科学基金(31172342);; 国家科技支撑计划(2013BAD12B05);; 国际合作项目(D32025/17453);; 国家公益性行业科研专项(200903037)
【CateGory Index】: S855.3
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