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《ACTA VETERINARIA ET ZOOTECHNICA SINICA》 1997-01
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STUDIES ON ELISA FOR AVERMECTIN B 1 RESIDUE IN CATTLE TISSUES

Li Junsuo 1, Qian Chuanfan 2 ( 1Department of Veterinary Pharmacology and Toxicology,College of Vet.Med., Beijing Agricultural University,Beijing 100094) ( 2Department of Applied Chemistry,Beijing Agricultural University,Bei  
A rapid and sensitive indirect competitive ELISA for avermectin B 1(AVM)residue was developed.The competitive conditions of ELISA were optimized in pH values,ionic strengths,and organic solvent.The ELISA can tolerate 20%(v/v)methanol.In PBS 0.5% Tween 20,the limits of detection for different C Ag were 0.02~0.2ng/ml for AVM,with half maximal inhibition(I 50 )1.8~4.0ng/ml.Samples (cattle plasma,muscle and liver)were extracted with methanol,and the extracts were determined by ELISA after diluted with PBS-0.5% Tween 20(1∶1,v/v).The I 50 of standard curve for AVM ranged from 3.6 to 30.2ng/ml for different samples.Limits of detection for AVM were 0.1~0.6μg/kg.At 6.0~60.0μg/kg spiking levels,Recoveries(%)were 87.9~108.8(95.0±8.0),and CV(%)were 2.8~16.9(10.0±5.2).This can be used as a rapid method for detection of AVM in animal tissues.
【CateGory Index】: S858.230.2
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