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《CHINESE JOURNAL OF ANIMAL AND VETERINARY SCIENCES》 1999-04
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STUDY ON THE ORIGIN AND DIFFERENTIATION OF THE GOAT BASED ON THE mtDNA DIVERSITY

Li Xianglong 1,2,3 ,Zhang Yaping 1,Chen Shengou 2, Zeng Fantong 2,Qiu Xiangpin 2,Liu Xiangmo 2 (1.Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology,Chinese Academy of Science,Kunming,650223; 2.Sic  
Fourteen restriction endonucleases recognizing 6 base pair,Apa Ⅰ,BamH Ⅰ,Bgl Ⅰ,Bgl Ⅱ,Dra Ⅰ,EcoR Ⅰ,EcoR Ⅴ,Kpn Ⅰ,Pst Ⅰ,Pvu Ⅱ,Sac Ⅰ,Sal I,Sma Ⅰ and Xho Ⅰ were used to investigate the mitochondrial DNA(mtDNA)diversity of 218 individuals of 18 native goat breeds from 12 provinces and autonomous region in China.The genetic distance between haplotypes and the polymorphic degree(π value)were also calculated according to the methods of Neis.The results indicated that 5 restriction endonucleases,BamH Ⅰ,Bgl Ⅱ,Dra Ⅰ,EcoR Ⅰ and Sal Ⅰ,were polymorphic,and 41 restriction sites and 18 restriction morphs which could be sorted into 6 haplotypes were detected.It was shown that haplotype Ⅰ(BamH Ⅰ B,Bgl Ⅱ A,Dra Ⅰ A,EcoR Ⅰ A and Sal Ⅰ A)and Ⅱ(BamH Ⅰ B,Bgl Ⅱ A,Dra Ⅰ A,EcoR Ⅰ A and Sal Ⅰ B)were two main haplotypes.It was inferred that the native goat breeds in China might origin from two different maternal ancestors.The average genetic distance between haplotypes and the polymorphic degree of populations were 0 00436 and 0 0487% respectively.It was also inferred that the genetic diversity and the differentiation of native goat breeds in China was low.
【Fund】: 国家科委基础司专项经费 国家杰出青年基金
【CateGory Index】: S852.2
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