Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Northwest University for Nationalities(Natural Science)》 2014-04
Add to Favorite Get Latest Update

Preparation and Preliminary Application of Agar Microspheres for Cell Cultures

CUI Meng-nan;LI Ming-sheng;ZHANG Jian;ZHANG Lei;MA Su-juan;LONG Shi-he;JIN Dong-wu;FENG Yu-ping;Engineering & Technology Research Center of for Animal Cells;Life Science and Engineering College of Northwest University for Nationalities;Life Science and Engineering College of Lanzhou University of Technology;  
4% agar solution was used as the water phase,a certain volume ratio of cyclohexane as oil phase,span- 80 as the emulsifier,the agar microspheres were prepared by emulsifying dispersion method. Then the chemical crosslinked method was used. At last,orthogonal method was used to optimize the process of Surface charge modification. The contrast research of characterization and cell culture between Cytodex1 microcarriers and agar microspheres. Results show that the optimal modification process is: Adding two times volume 4. 5mol / L of Na OH solution,then 2 times volume 2. 5 mol / L DEAE HCL solution into microspheres,stirring in the state of heating and 60 ℃,sealed reaction 4 h. Surface charge density of AGAR microsphere was 4. 00 tendency for 100 g,was almost of Cytodex1 microcarrier( 4. 05 tendency /100 g). When cells were stationary culture on the two kinds of microspheres,adherent rate and the extension of four types of cells all had advantage and disadvantage. BHK- 21 cells were in suspension culture,Cytodex1 microcarrier surface appeared "empty "phenomenon,and on AGAR microsphere surface,cells were uniformly. Culturing to 120 h,cells on the surface of the two kinds of microspheres were averaging 4. 5 × 107 cells,then cells on the surface of Cytodex1 the cells began to decline,while cells on surface of AGAR microsphere were still proliferation. Summarily,homemade gelatin microspheres to have material for cell culture conditions.
【Fund】: 教育部“长江学者和创新团队发展计划”项目(IRT13091);; 兰州市科技计划项目(2014-1-158);; 国家民委创新团队计划项目([2013]231号);; 国家外专项目(GDW20146200235)资助
【CateGory Index】: R318.08
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved