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《Animal Husbandry & Veterinary Medicine》 2009-03
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Genetically marking of Aeromonas hydrophila strains with green fluorescent protein gene

LI Jing,ZHANG Xiao-lu,LIU Yong-jie,LU Cheng-ping(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)  
Aeromonas hydrophila is a gram-negative bacterium which is widely distributed in aquatic environments.It has long been known as an opportunistic pathogen for a variety of aquatic and terrestrial animals,including humans.Hemorrhagic septicemia caused by A.hydrophila is a major infectious disease in aquacultures.In this paper,the recombinant plasmid pWSK129-gfp was constructed,and then transformed into the virulent strain J-1 of A.hydrophila(J-1GFP) and the avirulent strain 4332(4332GFP),respectively.The transformants have bright green performance under fluorescent microscope.The stability assay showed that after 4332GFP was passaged for 70 generation times,the stability rate of the recombinant plasmid was up to 100%,but only 15.1% in J-1GFP after 10 generation times.Genetically marking of A.hydrophila strains with gfp gene provides a simple and intuitive approach to investigate the interaction between the bacterial species and hosts.
【Fund】: 教育部“新世纪优秀人才支持计划”基金(NCET-07-0440);; 江苏省自然科学基金(BK2007155)
【CateGory Index】: S941
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Chinese Journal Full-text Database 1 Hits
1 WU Qian,YAN Fang,LIU Feng-bo (Shanxi Agricultural University,Taigu,030801;Chain);Research Progresss on Aeromonas hydrophila[J];Livestock and Poultry Industry;2010-02
Chinese Journal Full-text Database 5 Hits
1 WANG Xiao-li1,SHAO Wei-xing2,SHAN Hu1(1.College of Animal Science and Technology,Qingdao Agricultural University,Qingdao,Shandong,266109,China;2.China Animal Health and Epidemiology Center,Qingdao,Shandong,266071,China);Advance in Green Fluorescent Protein[J];Progress in Veterinary Medicine;2008-01
2 Lu Chengping Department of Veterinary Medicine, Nanjing Agricultural University, 210014;PATHOGENIC AEROMONAS HYDROPHILA AND THE FISH DISEASES CAUSED BY IT[J];Journal of Fisheries of China;1992-03
3 Wang Yang~2 Zhang Zhiyi~1 Yang Shengli~2 Wu Ruping~1 (Shanghai Institute of Materia Medica, chinese Academy of Sciences, Shanghai)~1 (Shanghai Center of Biotechnology, chinese Academy of sciences, Shanghai)~2;Cloning par Region and the Effect of par Region on Stability of pUC9[J];Chinese Journal of Biotechnology;1992-02
4 HUANG Shao-Song XU Run-Lin LU Yong-Jun~ ** (School of Life Sciences,Sun Yat-sen University,Guangzhou 510275);Use of Green Fluorescent Protein to Study the Relationship between Legionella pneumophila and Its Protozoan Host[J];Microbiology;2006-02
5 WANG Chun-lin,CAO Xue,LIU Xiao-dan,GUO Lu-pu,HU Ting,Ni Xiao-dan,LIU Yong-jie,LU Cheng-ping(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China);Detection of biochemical characters and extracellular proteases in the different isolates of Aeromonas hydrophila[J];Animal Husbandry & Veterinary Medicine;2008-06
Chinese Journal Full-text Database 10 Hits
1 HU Xiu-cai (College of Life Science, Xuzhou Normal University, Xuzhou, Jiangsu 221116);Study on the Characteristics of Aeromonas sobria Producing Pigment and Its Outer Membrane Proteins (OMPs) Pattern[J];Journal of Anhui Agricultural Sciences;2008-08
2 ZHANG Yu-fen et al(Department of Biological Science,North China Coal Medical College,Tangshan,Hebei 063000);Drug Sensitivity Test of Pathogen of Bacterial Septicemia of Carps[J];Journal of Anhui Agricultural Sciences;2008-30
3 GAO Jian-zhong (College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 200090);Comparison on Detecting Aeromonas hydrophila by Real-time Fluorescence PCR with Conventional PCR and Bacterium Culture[J];Journal of Anhui Agricultural Sciences;2008-31
4 ZHANG Yu-fen et al (Department of Life Sciences,North China Coal Medical College,Tangshan,Hebei 063000);Isolation and Identification of Aeromonas hydrophila from Carp[J];Journal of Anhui Agricultural Sciences;2008-31
5 GAO Jian-zhong et al(College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306);Application of Real-time PCR for Detection of Aeromonas hydrophila by Using MGB Fluorescence Probe[J];Journal of Anhui Agricultural Sciences;2009-19
6 ZU Guo zhang 1,YU Wei yi 1,LI Jin nian 1,YANG Qi chao 1,ZHOU Guang jun 2,LI Jia yun 3 (1.Department of Fisheries,Anhui Agricultural University,Hefei 230036; 2.Goose Breeding Farm of Liuan City;3.Aquatic Seed Multiplication Farm of Liuan City;Studies on Serveral Bacteriosis and the Pathogeny of Grass Carp[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2001-03
7 SUN Hong xiang,TAO Yong jun (College of Animal Science,Zhejiang University,Hangzhou 310029,China);Pharmacodynamic Study on Biebaoning against Disease in Soft shelled Turtle (Trionyx sinensis)[J];Fisheries Science;2001-04
8 ZHANG Xiao jun,CHEN Cui zhen,FANG Hai,HE Zhen ping,GONG Yuan fang (Department of Animal Science,Hebei Vocational Teachers College,Changli 066600,China);Immunity Effects Observation on Fish Inoculated by Antigen of Aeromona[J];Fisheries Science;2002-01
9 MEI Guang hai,ZHU Yong sheng,CAO Rong,TANG Zhi yong (Fisheries Technical Extension Station of Haian County,Haian 226600,China);A Preliminary Study on the Bacterial Pseudotuberculosis of Wintering Tilapia[J];Fisheries Science;2002-04
10 ZHOU Bi-jun, LI Yong-ming,WEN Gui-lan, HU Yuan-zhou (Department of Veterinary Medicine, College of Animal Science, Guizhou University, Guiyang 550025,China);The Identification and Isolation of a Pathogen Aeromonas hydrophila from Channel Catfish[J];Fisheries Science;2004-12
Chinese Journal Full-text Database 10 Hits
1 GUO Chuang,WANG Yongkun(College of Animal Husbandry and Veterinary Medicine,Yangzhou Univers ity,Yangzhou 225009,China);Advancement in Study on Aeromonas hydrophila[J];Fisheries Science;2003-06
2 DONG Chuan-fu, LIN Tian-long (Fish Disease Research Center of Animal Husbandry and Veterinary Medicine Institute, Fujian Academy of Agricultural Sciences, Fuzhou, Fujian 350003, China);Research progress on Aeromonas hydrophila[J];Fujian Journal of Agricultural Sciences;2003-04
3 LI Nan1,2,SUN Yi-min1,2,TIAN Li-ying1,LI Xiao-yue2,SHEN Hong-qi3,WANG Feng-min3,LI Quan-zhen3,CAO Jie-ying3,ZHAO Bao-hua2 (1.Department of Pharmaceutical Engineering,Hebei Chemical Engineering and Pharmaceutical College,Hebei Shijiazhuang 050026,China;2.College of Life Science,Hebei Normal University,Hebei Shijiazhuang 050016,China;3.Department of Diseases Supervision,Hebei Aquatic Product Office,Hebei Shijiazhuang 050011,China);The Isolation and Identification of Aeromonas hydrophila[J];Journal of Hebei Normal University(Natural Science Edition);2009-02
4 Wu Huimin1,2 Lin Wenhui2 Shi Cunbin2(1.Dalian Fisheries University, Dalian 116023, China;2.Pearl River Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510380, China);Research Process on Aeromonas hydrophila[J];Hebei Fisheries;2007-03
6 Chen Huaiqing and Lu Chengping(Department of Veterinary Medicine);STUDY ON THE PATHOGEN OF EPIDEMIC SEPTICEMIA OCCURRED IN CULTURED CYPRINOID FISHES IN SOUTHEASTERN CHINA[J];Journal of Nanjing Agricultural University;1991-04
7 Chu Weihua and Lu Chengping (Key Lab of Animal Diseases Diagnostic and Immunology,Ministry of Agriculture, Nanjing Agric Univ,Nanjing 210095);Pathogenicity of Aeromonas hydrophila extracellular protease[J];JOURNAL OF NANJING AGRICULTURAL UNIVERSITY;2000-02
8 LI Yue-hong1,ZHANG Pei-jun2,GUO Wei-sheng1,CUI Ying-ying1,WANG Xin1(1.Jilin Agriculture University,Changchun Jilin 130118;2.The Center of Examination and Monitor in Jilin Province,Chan-gchun Jilin 130000);Isolation and Identification of Aeromonas Hydrophila[J];Jilin Animal Husbandry and Veterinary Medicine;2007-11
9 SHEN Jin-yu(Zhejiang Institute of Freshwater Fisheries,Huzhou 313001,China);Research Progresss on Aeromonas hydrophila[J];Journal of Zhejiang Ocean University(Natural Science);2008-01
10 Xiong Yan Wang Yin Zhou Yu et al (College of Animal Science and Technology, Sichuan Agricultural University/Yaan, Sichuan, China 625014);STUDY ON THE PATHOGEN OF BACTERIAL INFECTIOUS DISEASE IN CHINESE SOFT-SHELLED TURTLE[J];JOURNAL OF SICHUAN AGRICULTURAL UNIVERSITY;1997-02
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 SUN An sai,HUANG Guang ming (Supervision station of Veterinary Hygiene of Gaoling,Xi'an,710200,China);New Development of Application of Reporter Genes in the Monitoring of Gene Transferation[J];Progress In Veterinary Medicine;2002-06
2 SUN De-hui~(1),DU Jun-zheng~(2),CHANG Hui-yun ~(2),CAI Xue-peng~(2)(1.College of Veterinary Medicine,Gansu Agricultural University,Lanzhou,Gansu,730070,China;2.Key Laboratory of Animal Virology of Ministry of Agricultural,State Key Laboratory of Veterinary Etiological Biology,Lanzhou Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730046,China);Construction and Expression of Enhanced Green Fluorescent Protein with Gene of 3ABC of Foot and mouth disease virus[J];Progress in Veterinary Medicine;2006-04
3 Sun Qihuan;Sun Peifang;Jin Lihua;Wu Jiannong Shanghai Fisheries University;ON THE PATHOGENIC BACTERIA OF THE HEMOLYTIC ASCITESOSIS OF ALLOGYNOGENETIC CRUCIAN CARP[J];Journal of Fisheries of China;1991-02
4 Lu Chengping Department of Veterinary Medicine, Nanjing Agricultural University, 210014;PATHOGENIC AEROMONAS HYDROPHILA AND THE FISH DISEASES CAUSED BY IT[J];Journal of Fisheries of China;1992-03
5 ZHANG Min, REN Hui-xia (Institute of Biochemical and Biotechnological Drug, Shandong University, Jinan 250012, China);Progress in Application of Report Gene[J];Food and Drug;2007-09
6 YANG Ying-Jun~(1,2), ZHOU Peng~1(1.National Key Biotechnology Laboratory for Tropical Crops, Institute of Bioscience and Biotechnology, CATAS, Haikou 571101, China;2.Department of Gardening & Forestry,Henan University of Science and Technology,Luoyang 471003, China);Advances in the Study on Marker Genes in Transgenic Plants[J];Hereditas(Beijing);2005-03
7 Yang Zheng-shi National Institute for the Control of Pharmaceutical & Biological Products,Beijing 100050;A Study on Pathogens during an Outbreak of Fish Disease,the Recovery of a New Species of Aeromonas as the Causative Agent[J];Chinese Journal of Microecology;1990-01
8 CHEN Mei-ling,CHEN Huan-chun~*,SONG Yun-feng,HUANG Hong-liang(Laboratory of Animal Virology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China);Relationship between PCV1-ORF2 fusion protein sequence and its nuclear localization[J];Chinese Journal of Preventive Veterinary Medicine;2005-06
9 Chen Huaiqing;Study on Pathogen of Bacterial Hemorrhagic Septicemia of Rice Eel (Monopterus albus)[J];Chinese Journal of Zoonoses;1991-04
10 YUAN Xiao-song,SHEN Ji-long,WANG Xue-long,DONG Hui-feng,JIANG Ming-sen(Department of Microbiology and Parasitology,and Provincial Key Laboratory of Genomic Research,Anhui Medical University,Anhui,Hefei 230032,China);Transient expression of enhanced green fluorescent protein(EGFP) gene in Schistosoma japonicum[J];Chinese Journal of Zoonoses;2006-01
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