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《Animal Husbandry & Veterinary Medicine》 2009-03
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Genetically marking of Aeromonas hydrophila strains with green fluorescent protein gene

LI Jing,ZHANG Xiao-lu,LIU Yong-jie,LU Cheng-ping(College of Veterinary Medicine,Nanjing Agricultural University,Nanjing 210095,China)  
Aeromonas hydrophila is a gram-negative bacterium which is widely distributed in aquatic environments.It has long been known as an opportunistic pathogen for a variety of aquatic and terrestrial animals,including humans.Hemorrhagic septicemia caused by A.hydrophila is a major infectious disease in aquacultures.In this paper,the recombinant plasmid pWSK129-gfp was constructed,and then transformed into the virulent strain J-1 of A.hydrophila(J-1GFP) and the avirulent strain 4332(4332GFP),respectively.The transformants have bright green performance under fluorescent microscope.The stability assay showed that after 4332GFP was passaged for 70 generation times,the stability rate of the recombinant plasmid was up to 100%,but only 15.1% in J-1GFP after 10 generation times.Genetically marking of A.hydrophila strains with gfp gene provides a simple and intuitive approach to investigate the interaction between the bacterial species and hosts.
【Fund】: 教育部“新世纪优秀人才支持计划”基金(NCET-07-0440);; 江苏省自然科学基金(BK2007155)
【CateGory Index】: S941
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