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《Animal Husbandry & Veterinary Medicine》 2018-08
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Effects of ZEA on reactive oxygen species and mitochondrial membrane potential in T lymphocytes of mice

ZHANG Xinyi;WU Yunping;CAI Guodong;ZOU Hui;YUAN Yan;GU Jianhong;LIU Xuezhong;LIU Zongping;BIAN Jianchun;College of Veterinary Medicine,Yangzhou University;Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses;  
This research was to reveal the immunosuppressive mechanism of zearalenone( ZEA) by studying the effect of ZEA on the reactive oxygen species( ROS) and mitochondrial membrane potential of isolated T lymphocytes in mice after the activation of concanavalin A( Con A). High purity mouse T lymphocytes were isolated by immunomagnetic beads,and Con A( 5 μg/m L) was used as a specific activator of T cells which were treated with different concentrations of ZEA for 24 h,and different experimental blank groups( cells only),the Con A group( cells+Con A),the 20 μmol/L ZEA group( cells + Con A + 20 μmol/L ZEA),and the 40 μmol/L ZEA group( cells + Con A + 40μmol/L ZEA) were set up. The flow cytometry was used to detect the levels of reactive oxygen species and mitochondrial membrane potential in mouse T lymphocytes. The results were as follows: Compared with the positive control group,the ROS level of the cells in the blank group was very low after activation for 24 h. Compared with the blank group,the level of ROS increased significantly when ZEA was 40 μmol/L( P0. 05). In addition,the cells were cultured for 24 h,after which,compared with the blank group,the mitochondrial membrane potential of T cells decreased significantly when ZEA was 40 μmol/L( P0. 01). To sum up: ZEA had effect on the normal redox of T lymphocytes in mice,and then influenced the normal function of T lymphocytes.
【Fund】: 国家重点研发计划支持项目(2016YFD0501208);; 扬州大学大学生学术科技创新基金项目(x20170774);; 江苏高校优势学科建设工程资助项目(PAPD)
【CateGory Index】: S859.8
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